Biomedical subjects

E Manet

Publications and source records attributed to E Manet.

22 records · Page 2Linked to original sources

PubMed1987

Structure and sequence of the Drosophila zeste gene.

The zeste gene of Drosophila affects the expression of other genes in a manner that depends on the homologous pairing of the chromosomes bearing the target gene. Zeste mediates transvection effects, the ability of one gene to control the expression of its homologous copy on another chromosome. We have determined the structure of the zeste gene and several mutants bearing partial deletions and the sequence of the z+, z1, zop6 and z11G3 alleles. The predicted zeste protein has an unusual structure including runs of Gln, Ala and alternating Gln Ala. Contrary to expectations the z1, zop6 and z11G3 mutations can each be attributed to single amino acid changes. The analysis of the mutants suggests that the zeste gene product is required for normal expression of at least some genes and we argue that za mutants may have residual function.

Amino Acid Sequence↗

PubMed1986

Both Epstein-Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter.

We have identified two Epstein--Barr virus (EBV) transacting factors which are involved in the transcriptional activation of EBV early promoters in latently infected Raji cells. In Raji cells, expression of the factor EB1 encoded by the open reading frame (ORF) BZLF1 is necessary and sufficient to disrupt latency. However, factor EB2 encoded by the ORF BMLF1- BSLF2 does not disrupt latency when expressed alone in Raji cells. Expression of an EBV activatable early promoter depends on the presence of both EB1 and EB2.

Cell Line↗

PubMed1985

Isolation and characterization of the zeste locus of Drosophila.

The zeste gene of Drosophila regulates the expression of certain other genes like white, bithorax and decapentaplegic in a manner dependent on chromosome pairing. The genetic evidence suggests that its product interacts with the genes at the level of transcription. We have cloned the zeste region from fragments microdissected from the 3A1-4 region of the X chromosome and mapped the breakpoints of several rearrangements which localise the gene. We have isolated dysgenic zeste mutants which contain P element sequences inserted in the same region. DNA rearrangements were found in some zeste mutants but were not detected in the z, z or z mutants. The changes are localised to a Bam 4.0-kb fragment from which originates a 2.4-kb RNA species. This transcript is altered in some zeste mutants but is not visibly affected in Z or z mutants. The region bears no homology to the white gene or its vicinity but cross-hybridises to many other genomic sequences in Drosophila.

Journal Article↗

PubMed1985

Construction and use of cDNA clones for the mapping and identification of Epstein-Barr virus early P3HR-1 mRNAs.

cDNA clones, specific for early Epstein-Barr virus (EBV) RNAs, were constructed from total cytoplasmic RNA of P3HR-1 TK- cells. From 10,000 cDNA clones screened, 22 virus-specific cDNA clones were selected by hybridization with a total EBV DNA. These clones were then precisely mapped on the EBV genome and the corresponding mRNAs were identified by Northern blot hybridizations. Most of them are clearly related to some of the open reading frames described by Baer et al. (Nature [London] 310:207-211, 1984). They represent at least 18 different genes active during the early viral cycle. The transcriptional activity of the virus during the early stage was also studied by dot blot hybridization of total early cDNA probe to EBV genomic fragments. Three main regions showed very strong hybridization with the cDNA probe: BamHI a, M, and L fragments, BamHI K, B, and G fragments, and BamHI B1 fragment (deleted in strain B95-8) and the adjacent right end of the DNA molecule. Seventeen of the cDNA clones were localized in these highly transcribed regions. The five others were dispersed all along the EBV genome.

Animals↗