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Biomedical subjects

E McLaughlin

Publications and source records attributed to E McLaughlin.

17 recordsLinked to original sources
PubMed2004

Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis.

Rabbit IL-4 was expressed in the virulent standard laboratory strain (SLS) and the attenuated Uriarra (Ur) strain of myxoma virus with the aim of creating a Th2 cytokine environment and inhibiting the development of an antiviral cell-mediated response to myxomatosis in infected rabbits. This allowed testing of a model for genetic resistance to myxomatosis in wild rabbits that have undergone 50 years of natural selection for resistance to myxomatosis. Expression of IL-4 significantly enhanced virulence of both virulent and attenuated virus strains in susceptible (laboratory) and resistant (wild) rabbits. SLS-IL-4 completely overcame genetic resistance in wild rabbits. The pathogenesis of SLS-IL-4 was compared in susceptible and resistant rabbits. The results support a model for resistance to myxomatosis of an enhanced innate immune response controlling virus replication and allowing an effective antiviral cell-mediated immune response to develop in resistant rabbits. Expression of IL-4 did not overcome immunity to myxomatosis induced by immunization.

Animals↗
PubMed2002

British Andrology Society guidelines for the assessment of post vasectomy semen samples (2002).

The British Andrology Society guidelines for the assessment of post vasectomy semen samples recommend that initial assessment is undertaken 16 weeks post vasectomy and after the patient has produced at least 24 ejaculates. The laboratory should examine a freshly produced seminal fluid specimen by direct microscopy and if no sperm are seen the centrifugate should be examined for the presence of motile and non-motile spermatozoa. It is recommended that the clinician should give clearance after the production of two consecutive sperm free ejaculates. In cases of persistent identification of non-motile spermatozoa the referring clinician should advise the patient regarding the cessation of other contraceptive precautions. Surgeons are responsible both preoperatively and postoperatively for the counselling of couples regarding complications and the possibility of late recanalisation after clearance.

Fertility↗
PubMed2000

The load-displacement characteristics of neonatal rat cranial sutures.

OBJECTIVE: Recently several centers have attempted to distract the craniofacial skeleton in infants with craniosynostosis. To effectively achieve this goal, we must first understand the normal sutural response to tensile forces. The objective of this study was to determine the load-displacement characteristics of neonatal rat sutures. METHODS: Thirty cranial sutures were harvested from 1-week-old Wistar rats (10 each coronal, posterior frontal, and sagittal). The width of the harvested bone-suture-bone construct was standardized to 4 mm. The specimens, kept moist, were mounted fresh and distracted at 10 microm/sec until rupture using a Vitrodyne V1000 universal tester. Standard load-displacement curves were constructed. The stiffness, defined as tensile force/change in suture length, and the ultimate stress, defined as tensile force at suture rupture/cross sectional area, were calculated. RESULTS: These sutures demonstrated classical viscoelastic behavior. During the elastic phase, they elongated approximately 1 microm for every 1 g of force (10(4) N/m). The ultimate tensile stress was approximately 4 MN/m2. The estimated mean elastic modulus was 10 megapascals. The posterior frontal sutures were significantly less stiff than the other two sutures (Kruskal-Wallis nonparametric analysis of variance, p = .0023). The difference in the ultimate stress was also significant (p = .0201). CONCLUSIONS: This study provides data regarding the basic mechanical behavior of neonatal cranial sutures in a mammalian system.

Analysis of Variance↗
PubMed2000

Dose of selective serotonin uptake inhibitors across pregnancy: clinical implications.

The use of antidepressants during pregnancy has undergone considerable scrutiny with respect to safety issues, though limited data with respect to dose management and symptom resolution is available. Previous reports on tricyclic antidepressants (TCAs) have demonstrated the need to adjust maternal dose later in pregnancy to maintain therapeutic serum concentrations. However, there is no data on the dosage of selective serotonin uptake inhibitors (SSRIs) required to maintain symptom resolution in women treated for major depression during pregnancy. The purpose of this study, then, was to assess the medication dosage requirements of SSRIs during this time. In this naturalistic study, pregnant women with a primary diagnosis of major depression were followed prospectively through pregnancy at monthly intervals with symptom assessment. Subjects were included in data analysis if they presented prior to 28 weeks gestation, were treated with SSRI monotherapy, received all psychiatric treatment during the pregnancy at the Emory Pregnancy and Postpartum Mood Disorders Program, and achieved euthymia after initial treatment intervention (CGI = 1 and Beck Depression Inventory (BDI) < 9) during pregnancy or failed to respond after eight weeks of treatment. Medication selection was based on personal treatment history or family treatment history (if any), and the published data on SSRIs in pregnancy. All medication dose adjustments were based on depressive symptoms as measured by the BDI and a psychiatric interview (ZNS). Thirty-four pregnant women were included in final analysis. Two thirds of the subjects (n = 22) required an increase in their daily dose of medication to maintain euthymia. The dose increases occurred at 27.1 +/- 7.1 weeks gestation, with mean BDI scores of 16.4 +/- 9.6, compared to a mean treatment response BDI of 6.9 +/- 5.4. Subject's age, education, past personal and familial psychiatric history were not significantly associated with dose adjustment. These novel data on SSRI daily dose in pregnancy parallels the extant literature with tricyclic antidepressants (TCA). Further work to determine the predictors of dose adjustments will provide guidelines for minimizing fetal exposure to both medication and maternal mental illness.

Major Depressive Disorder↗
PubMed1997

The application of various protic acids in the extraction of (1-->3)-beta-D-glucan from Saccharomyces cerevisiae.

Glucans are (1-->3)-beta-linked glucose polymers which have immune-stimulating capability. The extraction of water-insoluble (1-->3)-beta-D-glucan form Saccharomyces cerevisiae employs hydrochloric acid. Hydrochloric acid is difficult to employ in the large-scale pharmaceutical extraction of glucans due to its corrosive nature and toxicity. To address these concerns, we determined whether acetic, formic or phosphoric acid can be substituted for hydrochloric acid in the process for the isolation of (1-->3)-beta-D-glucan. The resulting microparticulate glucans were employed as the starting material for the production of (1-->3)-beta-D-glucan phosphate. 13C NMR analysis of the glucan phosphates derived from the acetic, formic or phosphoric acid-extracted microparticulate glucan show excellent correspondence to hydrochloric acid extracted glucan and laminarin, a (1-->3)-beta-D-glucan standard, indicating that the primary structure is not altered by the acid used for extraction. Glucan phosphate prepared from hydrochloric acid had a Mw of 7.2 x 10(4) g/mol, rmsz of 17.7 nm, of 1.50 and (eta) of 49.0 mL/g. Glucan phosphate prepared from acetic acid had a primary polymer peak with a Mw of 1.4 x 10(6) g/mol, rmsz of 23.6 nm, I of 1.93 and (eta) of 62.4 mL/g. Glucan phosphate prepared from formic acid had a main polymer peak with a Mw of 1.2 x 10(6) g/mol, rmsz 27.1 nm, I of 1.56 and (eta) of 89.0 mL/g. Glucan phosphate prepared from phosphoric acid had a primary polymer peak with a Mw of 6.6 x 10(5) g/mol, rmsz of 32.3 nm, I of 2.70 and (eta) of 91.3 mL/g. These data indicate that the molecular mass, size, polydispersity and intrinsic viscosity of the glucan phosphate obtained is influenced by the pKa of protic acid employed to extract the microparticulate glucan. However, the primary structure and side-chain branching are not substantially altered regardless of the acid employed.

Acetic Acid↗
PubMed1997

Integrating relationship constructs and emotional experience into false belief tasks in preschool children.

Themes of separation from attachment figures are involved when caregivers are integrated into standard theory of mind tasks in which objects or toys are located. Two experiments test the hypothesis that searching for a caregiver would interfere with false belief performance and be related to a child's emotional awareness. Experiment 1 consisted of a cross-sectional study of three- to five-year-old children administered false belief tasks related to object identity, object location, and caregiver location, i.e., false belief tasks where story characters became separated from a parent and had to locate them. As expected, there were age-related improvements in false belief performance to above-chance levels during object identity and object location tasks, but performance on the caregiver location tasks showed no age-related improvement and at age five was poorer than other tasks. Emotional integration also varied with task. Children who were relatively more aware of emotions were more likely to pass tasks involving objects, and queries of emotions during tasks were related to false beliefs about objects but not caregivers. A second study of children five years of age indicated that it was not caregivers per se that disrupted their performance on false belief tasks. Additional tasks showed that this finding was due to caregivers being animate "behaving" objects whose relocation had been self- as opposed to other-directed, which suggests that false belief performance was related to the intent of the sought item. The developing awareness of the minds of others in five-year-olds and emotional content of the task may interfere with performance in false belief tasks that are social.

Affect↗
PubMed1987

Attitudes towards donor insemination--a post-Warnock survey.

In a multi-centred study, a total of 799 patients, donors and health-care professionals concerned with artificial insemination with donor semen (AID) responded to a questionnaire regarding their attitudes towards current provision of AID services and proposed legislation. There was little support for any fundamental change in the way in which AID is practised, at least in those centres. The anonymous status of the donor met with universal agreement. Although there was some support for the communication of non-identifying details to the recipient couple, where they wanted them, there was no support for any legislation which might give the AID child a right of access to details of the donor. The greatest divergence of opinion was over the question of who should have access to AID treatment and whether or not screening procedures should be applied to prospective parents. Most respondents felt that the closed and confidential relationship between the clinic and the other parties involved should not automatically be extended to general practitioners or any national bodies. In respect of specific recommendations of the Warnock Committee, there was support for changes which might legitimize or assist the present system, but not for any which might be restrictive.

Attitude↗
PubMed1987

A controlled trial of treatments for generalized anxiety.

Treatments for chronic anxiety have received considerable interest recently. Firstly, there is a body of research which has shown problems of dependence and habituation in the long-term use of anti-anxiety drugs. A second development is that of psychological treatments for anxiety. Because of the problems involved in the use of benzodiazepines, it is important to determine whether or not psychological treatments are a reasonable alternative when treating chronic anxiety. This study was designed to test the relative effectiveness of cognitive-behavioural therapy, anxiety management training and treatment by benzodiazepines against a waiting list control. Measures were taken on both the process and outcome of treatment. The most immediate and greatest improvements in anxiety were seen in the group receiving drugs. However, these improvements reduced as the trial progressed and were minimal at the end of therapy. Both psychological treatment groups improved as the trial progressed with the most significant and consistent changes seen in the cognitive-behaviour therapy group. However, at follow-up there was no difference between the two groups receiving psychological treatments. Because of their lack of sustained improvement, over half of the group receiving drugs refused to wait without treatment until the follow-up assessment.

Adult↗
PubMed1979

Pulmonary function in firefighters: acute changes in ventilatory capacity and their correlates.

A group of 39 firefighters was examined during routine firefighing duty. Following smoke exposure the average decrease in one-second forced expiratory volume (FEV1.0) was 0.05 litre (137 observations). This decline in FEV1.0 was related to the severity of smoke exposure as estimated by the firefighter and to the measured particulate concentration of the smoke to which he was exposed. Decreases in FEV1.0 in excess of 0.10 litre were recorded in 30% of observations. Changes in FEV1.0 resulting from a second exposure to smoke on the same tour of duty were greater when smoke exposure at the previous fire was heavy. The repeated episodes of irritation of the bronchial tree that have been documented in this investigation may explain the origin of the previously observed chronic effect of firefighting on respiratory symptoms and pulmonary function.

Adult↗
PubMed1977

Patterns of high tension electrical injury in children and adolescents and their management.

High tension electrical burn injury occurred in twenty-nine patients over a period of seven years, causing two deaths, and demonstrating two reasonably distinct patterns. Major surface thermal burns from involvement in an electric arc, augmented by flame burns of ignited clothing, occurred in eleven patients. By contrast, eighteen children demonstrated wounds of entrance and exit of current and varying effects of deep thermal injury along the path of the maximally conductive blood vessels and nerves of extremities. An aggressive surgical approach was taken in patients with both types of injury, with the objective of prompt identification and excision of devitalized tissues and closure of thw wound. In the deep condictuve type of injury, frozen section and subsequent histologic study served as a guide to adequacy of excision and preservation of viable tissue. Even so, amputation of fifteen extremities and four other major excisions were required in twelve patients.

Adolescent↗
PubMedDate not supplied

A surgical approach to a displaced ankle fracture.

The goal of reduction in displaced ankle fractures is the restoration of normal function in the involved extremity. This can be accomplished only by restoring the normal anatomy of the involved joints. Closed reduction is not always successful or indicated if vascular compromise, interspersed soft tissue, or osseous fragments are present. Open reduction with internal fixation allows for direct visualization of the involved parts and an accurate and rigid form of fixation. A case report of a displaced ankle fracture treated with open reduction and internal A-O fixation is presented.

Ankle Injuries↗