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Biomedical subjects

E N Kirillova

Publications and source records attributed to E N Kirillova.

At least 19 recordsLinked to original sources
PubMed1989

[Hematopoietic precursor cells in Wistar rats with acute myeloblastic leukemia].

The studies were performed on Wistar rats with acute myeloblastic leukemia induced by a prolonged administration of tritiated water (370 MBq/kg/day-1; an absorbed dose--25.3 Gy) and by repeated pyrogenal injections. A reduction of the pool of polypotent hemopoietic cells (CFUs) with a simultaneous increase of their proliferative activity and a decline of their ability to self-supporting were observed in the leukemia-bearing animals. Populations of CFUdc in DC and of CFUc were reduced in all cases; their colony-forming ability was also found to decrease. All animals with leukemia showed a lowered amount of CFU-BL in the bone marrow and of CFU-TL in the thymus, although the content of these cells in the peripheral blood remained unchanged.

Animals↗
PubMedDate not supplied

[The cytotoxic activity of natural killer cells exposed to the joint and separate actions on the body of 239Pu, hexachlorobutadiene and tributyl phosphate].

Wistar rats were subjected to a single exposure to 239Pu nitrate through inhalation with the subsequent procedure of imitation of inhalation or without it (the amount of 239Pu deposited in the lungs in 24 hr was 8.9 +/- 1.9 kBq/lung) and inhalation of hexachlorobutadiene and tributyl phosphate within one month in a combination with the radionuclide or without it. There was a 1.5-fold increase, above additive, in the harmful effect of 239Pu and chemical agents on the function of natural killers as observed 15--30 days after the combined exposure as compared to individual inhalation. On days 120 to 240 cell cytotoxic activity in rats of all groups was normalized to reach or to exceed the intact control.

Administration, Inhalation↗
PubMedDate not supplied

[The effect of granulocyte-macrophage colony-stimulating factor on hemopoiesis recovery and the survival of irradiated mice].

A human recombinant granulocytic-and-macrophagic colony-stimulating factor (rGM-CSF) administered repeatedly to irradiated (10 Gy) CBA mice increased CFUs and CFU-GM content, the number of bone marrow granulocytes and erythronormoblasts, and spleen and peripheral blood cellularity. The survival rate of exposed (9.7 Gy) mice repeatedly injected with rGM-CSF increased from 25% (control) to 90%.

Animals↗
PubMedDate not supplied

[The early changes in humoral immunity under the prolonged action of tritium oxide with different dose rates].

Within the dose range from 0.2 to 1 Gy, early changes in the humoral immunity of mice exposed to tritium oxide at varying dose rates have been investigated. The study of the immunity impairment at different stages of immunopoiesis permits to reveal the points (a lymphocyte precursor department) that are mostly affected by radiation, to find the causes of the decrease in the antibody production, and to reveal the relationship between the damages observed the dose absorbed and dose rate of beta radiation.

Animals↗
PubMedDate not supplied

[The immunity indices of rats after the long-term action of tritium oxide or gamma irradiation].

In experiments with Wistar rats, a study was made of the content of antibody-forming cells and cytotoxic activity of normal killers after long-term administration of tritium oxide (3HOH) (370 kBq.g-1 of body mass daily, cumulative dose, 8.1 Gy, and dose rate, 8.5 cGy/day), and after gamma irradiation with corresponding doses. The long-term radiation effect caused a decrease in the immunity indices: the impairment of the immune reactions was more pronounced after the effect of 3HOH than after gamma irradiation. Damages to the immune system of mice and rats after irradiation with similar doses were compared.

Animals↗
PubMedDate not supplied

[The significance of immunity disorders in the development of tumorous effects during the long-term action of tritium in mice].

In experiments with CBA mice it was shown that the long-term influence of tritium oxide administered with drinking water daily during 180 days (370 kBq.g-1 of body mass, cumulative dose 8.7 Gy, dose rate 4.5 cGy.day-1) causes the development of immunity deficiency 90 days after the onset of the administration that persists for up to 270 days. There is a 34% decrease in the average life of irradiated animals and an increase in the number of malignant neoplasms (postmortem examination was performed after natural death or killing on days 250, 350 and 450). There is a direct relationship between the occurrence of malignant neoplasms and the immunity deficiency.

Animals↗
PubMedDate not supplied

[The antibody-producing cell count of rats exposed to polymeric 239Pu].

In experiments with Wistar rats a study was made of the content of antibody-forming cells (AFC) in the spleen at remote times (3 to 12 months) after intravenous injection of 239Pu(IV) in doses of 166, 55, and 18 kBq/kg body mass. The doses absorbed in the central and peripheral immunity organs were defined. Pronounced spleen hypoplasia and profound inhibition of humoral immunity were displayed 1 year after the injection of a small amount of the radionuclide. AFC deficiency in animals was amounted to 11-32 per cent throughout the entire period of observation.

Animals↗
PubMedDate not supplied

[Effect of prolonged continuous external irradiation on humoral immunity indices of mice].

Changes in the content and function of cell populations and subpopulations involved in the humoral response of mice to the thymus-dependent antigen were investigated. The effect was followed during a prolonged continuous exposure to 137Cs gamma-emitter (total dose--5 Gy and daily dose--12 cGy for 22 hours) and after its termination. The data obtained give evidence for a decrease of the pool of polypotent lymphocyte precursors (CFUs), stable moderate hypoplasia of central and peripheral organs of the immune system, distinct inhibition of antibody production at the expense of reduced activity of precursors of lymphocytes, B-lymphocytes and T-helpers. In the remote postirradiation period residual radiation damage was seen in polypotent and committed precursors of lymphocytes and T-helpers which was responsible for the trend towards the decline of antibody production, hypoplasia in the spleen and lymph nodes being persistent.

Animals↗
PubMedDate not supplied

[Functional activity of cellular systems of immunity a long time after sublethal irradiation].

A study was made of the content and functional activity of cell populations and subpopulations involved in the immune response at remote times after single sublethal irradiation (3 Gy). The exposed mice exhibited an earlier thymus involution and a decrease in the number of CFUc and antibody-producers per 10(6) karyocytes. The decrease in the antibody production was due to the reduced functional activity of B- and T-lymphocyte precursors and cooperative activity of T-helpers. The inactivating capacity of T-killers increased.

Animals↗
PubMedDate not supplied

[Cloning of murine B- and T-lymphocytes in vitro after the long-term action of tritium oxide].

The cloning method was used to study the content of B- and T-lymphocyte committed precursors in central and peripheral organs of the immune system of mice at different times after long-term exposure to tritium oxide (a cumulative dose of approximately 9 Gy). It was shown that recovery of the colony-forming ability of the committed lymphocyte precursors was different in central and peripheral lymphoid organs; the dynamics and degree of restoration of the pools of B--(CFU--LB) and T--(CFU--LT) lymphocyte precursors were different.

Animals↗
PubMedDate not supplied

[Inactivation of non-syngeneic hematopoietic stem cells by mouse lymphocytes after long-term exposure to tritium oxide].

A study was made of functional activity of lymphocytes, which inactivated nonsyngeneic colony-forming units (CFU) in allogenous and xenogenous interaction systems, at different times after long-term action of tritium oxide in a cumulative dose of approximately 9 Gy. The function of these lymphocytes was depressed during the first months after termination of exposure while at later times it exceeded the control level. The consequences of the interaction between lymphocytes and nonsyngeneic CFU were also studied in this work.

Animals↗
PubMedDate not supplied

[Normal killer function in CBA mice as affected by long-term intake of tritium oxide].

A study was made of the cytotoxic function of normal killers of CBA mice during long-term administration of tritium oxide in potable water (a cumulative absorbed dose of 8.7 Gy, dose-rate, 4.5 Gy/day) and at different times after termination of the radionuclide injection. A mean decrease of 20-30% in the lytic effect of the effectors from spleen of the experimental mice on cell-targets of K-562 human erythroleukosis was demonstrated by a 17-19-hour test with 51Cr. At later times after termination of action of tritium oxide, the cytotoxic effect of normal killers increased by 1.8 times as compared to intact controls of the same age.

Animals↗
PubMedDate not supplied

[Recovery of the cellular immunity system after sublethal radiation].

Dynamics and recovery of quantitative and qualitative characteristics of lymphoid tissue were studied after sublethal irradiation. The data obtained are indicative of a different dynamics of recovery of cells in central and peripheral lymphoid organs, GFUs and antibody-forming elements. The peculiarities have been revealed in a change of functional capacity of some T-lymphocyte populations.

Animals↗
PubMedDate not supplied

[Long-term effects of sublethal radiation doses and modifying factors on the state of immunological indices in mice].

A study was made of mean life, amount of CFUs and plaque-forming cells, and functional activity of T-lymphocyte subpopulations in CBA mice a year after irradiation with a dose of 3 Gy and a single therapeutic administration (after 4-5 h) of bone marrow and thymus cells, or after shielding of femur during exposure. The effects obtained after irradiation at different dose-rates (0.031 and 1.29 mA/kg) have been compared.

Animals↗