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Biomedical subjects

E N Mendes

Publications and source records attributed to E N Mendes.

At least 55 records · Page 3Linked to original sources
PubMed1991

Helicobacter pylori acute gastritis: histological, endoscopical, clinical, and therapeutic features.

We describe seven patients with Helicobacter pylori acute gastritis who presented endoscopical picture simulating gastric carcinoma or lymphoma. H. pylori was detected on the antral and on the oxyntic mucosa of all patients. At histological examination, no evidence of malignancy was observed in any of the patients. In the antral mucosa there were edema, hyperemia, and intense polymorphonuclear leukocyte infiltration (neutrophils and some eosinophils) in the lamina propria, mucus layer, and inside the glands. The patients were treated with amoxycillin (2 g/day for 2 months) associated with metronidazole (750 mg/day for 15 days), which reduced the symptoms dramatically. Endoscopy and biopsy, repeated 4 wk after treatment was stopped, showed that the patients had been cleared of H. pylori, and indicated a marked improvement of endoscopical and histological gastritis. These findings indicated that H. pylori acute gastritis can mimic gastric carcinoma or lymphoma. Therefore, histology and detection of H. pylori are essential for an accurate diagnosis.

Acute Disease↗
PubMed1990

A spiral microorganism in the stomach of pigs.

Light and electron microscopic and microbiologic evaluations were performed on mucosa of stomachs from 120 healthy slaughtered pigs. Helicobacter pylori was not found, but a tightly spiralled bacterium, not previously described, was seen in histological sections and/or in carbol fuchsin stained smears in 13 (10.8%) stomachs. In paraffin sections stained with carbol fuchsin, the bacteria were seen in the mucus of the lumen of the antral pits and in the mucosa surface within and beneath the mucus. In this sections of Polilyte embedded tissue the bacteria had three to eight spiral turns per cell (mean = five), flattened ends, a Gram-negative cell-wall structure and a sheathed flagella. The urease test was positive in gastric mucosa of 13 bacteria-positive pigs (10.8%). The microorganism was not cultured and did not cross-react with polyclonal antibodies raised in rabbits against H. pylori. Superficial chronic gastritis and "borderline" gastritis were observed in antral mucosa of 10 (76.9%) and of two (15.4%) spiral bacteria-positive pigs, respectively.

Animals↗
PubMed1990

Ultrastructure of a spiral micro-organism from pig gastric mucosa ("Gastrospirillum suis").

The ultrastructural features of a helical-shaped bacterium occurring in the stomach of pigs, within the mucus on the mucosal surface of antral pits, were examined. The bacterial cell had three to eight spiral turns, flattened and truncated ends and was approximately 4.0 microns long and 0.6 microns wide. In some sections, up to six flagella, about 22 nm in diameter, were seen arising from each pole. The cytoplasm contained sparse, irregular granules, numerous ribosomes and large single-layered membrane-bound granules. In the flagella insertion area, there was a highly electron-dense component, the "polar membrane". This micro-organism differed from similar bacteria described in cats, dogs and monkeys, and may cause inflammation in the antral mucosa of pigs similar to Helicobacter pylori infection in man. Furthermore, it was morphologically similar to the spiral micro-organism distinct from H. pylori which has been described recently in human antral mucosa from patients with gastritis and may be of potential significance as a pathogen in man. The name "Gastrospirillum suis" is proposed for this bacterium.

Animals↗
PubMed1990

New invasive serotype of Escherichia coli.

A new invasive Escherichia coli strain which presented delayed or variable reactions in lactose, mucate, and acetate tests is described. It did not agglutinate with antisera for all known E. coli and Shigella groups A, B, C, D, and provisional Shigella serovar. We propose the designation of E. coli BH until the situation of its 0 antigen is settled.

Antigens, Bacterial↗
PubMed1990

[14C-urea breath test to diagnose gastric Helicobacter pylori colonization].

Helicobacter pylori (H. pylori), ex-Campylobacter pylori, is now considered to be the causative agent of active chronic gastritis in humans and may be diagnosed by histology, gram-stain, culture, urease test or noninvasively by 14C-urea breath test. The aim of this study was to determine the sensitivity, specificity, and reproducibility of the breath test as compared with the microbiological (culture) "gold standard". Forty-one subjects were studied. Gastroscopy was performed in all participants and 2 antral mucosa biopsy specimens were taken for urease test and microbiological (gram-stain and culture) studies. The breath test was performed within one week after the gastroscopy. After fasting overnight, a control breath sample was obtained and a standard meal was offered to delay gastric emptying. Then, the participants drank 5 microCi of 14C-urea in 20 ml of water and breath samples were collected at 10, 15, 30 and 60 min in a hyamine-ethanol solution with a p-H indicator. A repeat test was performed in 9 subjects to assess test reproducibility. Twenty-nine subjects (71 percent) were Hp positive and 12 (29 percent) H. pylori negative. A highly significant difference (p less than 0.0001) was observed in 14CO2 exhalation in colonized and non-colonized subjects in samples collected at all times studied. As compared with culture we found that the breath test was 97 percent sensitive and 100 percent specific. We conclude that 14C-urea breath test is a simple, inexpensive, sensitive, and reliable method to detect H. pylori colonization. It is a useful test to epidemiologic and therapeutic trials.

Adult↗
PubMed1988

Distribution of Campylobacter pylori and gastritis in the stomach of patients with and without duodenal ulcer.

The presence of Campylobacter pylori and histological changes of gastric mucosa were studied in 50 consecutive patients with gastric complaints. C. pylori was isolated from the antrum, body, and fundus of 40 patients with (n = 20) and without (n = 20) duodenal ulcer. The incidence of gastritis was not significantly different in the antrum of C. pylori-positive patients with and without ulcer. Otherwise, oxyntic mucosa of both body and fundus regions exhibited gastritis in 64.1% of the C. pylori-positive non-ulcer patients, whereas those with duodenal ulcer presented oxyntic mucosa that was histologically normal or near normal.

Adult↗
PubMed1988

Immunocytochemical identification of Campylobacter pylori in gastritis and correlation with culture.

Endoscopic biopsy specimens of antral mucosa from 25 patients presenting with gastric complaints were obtained for culture and histologic and immunocytochemical studies. The histopathologic study revealed chronic gastritis in 22 patients and borderline chronic gastritis in three patients. The unlabeled-antibody peroxidase-antiperoxidase (PAP) method was applied for the detection of Campylobacter pylori, and its results were compared with those obtained with the culture technique. Strongly positive immunoperoxidase staining was localized in spiral, curved bacteria that were present in the mucus layer adjacent to the gastric epithelial cell surface. The microorganisms were frequently congregated in clumps and were sectioned in several directions. The PAP stains were positive in 19 specimens (76%), and the cultures were positive in 20 (80%). All results negative by culture were also negative by PAP method. Compared with the cultures, the sensitivity and positive predictive value of the PAP method for identification of C pylori in antral mucosa obtained from endoscopic biopsy specimens were 95% and 100%, respectively.

Biopsy↗
PubMed1987

Indicator medium for isolation of Campylobacter pylori.

The use of a new indicator culture medium, Belo Horizonte medium, is proposed for better colony recognition and a presumptive identification of Campylobacter pylori. This medium, containing brain heart infusion sheep blood agar, was supplemented with 40 mg of 2,3,5-triphenyltetrazolium chloride per liter in addition to vancomycin, nalidixic acid, and amphotericin B. On Belo Horizonte medium, Campylobacter pylori present unique golden colonies.

Biopsy↗
PubMedDate not supplied

Identification of a new enteroinvasive Escherichia coli strain.

We isolated an enteroinvasive Escherichia coli strain which did not react with antisera raised against all known E. coli serogroups, Shigella groups A, B, C and D or provisional Shigella serotypes. We propose the provisional designation of Escherichia coli MG for this strain, until the identification of its O antigen is established.

Adult↗
PubMedDate not supplied

Chicken carcasses as a source of Campylobacter jejuni in Belo Horizonte, Brazil.

The presence of Campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in Belo Horizonte. In the latter C. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. In the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of the 40 stool specimens. There was a significant difference between industrial and non industrial slaughter in regard to the frequency of C. jejuni isolation from carcasses (p = 0.000002), probably due to the low hygiene conditions present in non industrial slaughters. The results of antimicrobial susceptibility tests, SDS gel electrophoresis and biotyping of the strains isolated from stool specimens obtained from chicken meat workers were similar to those observed in strains isolated from chicken carcasses which suggest that chicken could be the source of C. jejuni for the workers and both, chicken and workers, could be implicated in the transmission of C. jejuni infection in Belo Horizonte.

Animals↗
PubMedDate not supplied

Chronic gastritis and Helicobacter pylori in digestive form of Chagas' disease.

Patients with the digestive form of Chagas' disease frequently present chronic gastritis. As the microorganism Helicobacter pylori is now accepted as the most common cause of human chronic gastritis, the present work was undertaken to verify a possible relationship between the presence of this bacterium and inflammatory changes of antral mucosa in chagasic patients. Seventeen chagasics, with megaesophagus and or megacolon were studied. Fragments from two different regions of antral mucosa were obtained by endoscopy, fixed in 4% neutral formaldehyde and embedded in paraffin. The sections were stained by haematoxylin and eosin for histology analysis, and by carbolfuchsin for H. pylori identification. H. pylori was found in 16 (94.1%) chagasic patients, all of them presenting chronic gastritis. Superficial gastritis was seen in 9 (52.9%) while atrophic gastritis was present in 8 (47.1%) patients. H. pylori was present on gastric mucosa of 8 (100%) patients with atrophic gastritis and of 8 (88.8%) patients with superficial gastritis. We concluded that the microorganism H. pylori should be considered a possible factor connected with the etiopathogenesis of chronic superficial and atrophic gastritis frequently observed in patients with the digestive form of Chagas' disease.

Adult↗
PubMedDate not supplied

Prevalence of Helicobacter pylori infection in a rural area of the state of Mato Grosso, Brazil.

The prevalence of Helicobacter pylori infection was evaluated by ELISA in 40 children and teenagers and in 164 adults from a rural area of the State of Mato Grosso, Brazil. Antibodies to H. pylori were detected in the serum of 31 (77.5%0 children and teenagers and in 139 (84.7%) adults. The prevalence of infection increased with age (x2 for trend, p < 0.01) even though no variations occurred in the region in the present century in terms of living conditions or sanitation, economical development and migratory influx supporting the hypothesis that the infection is also acquired during later life in developing countries. An inverse correlation was observed between the prevalence of infection and annual family income (x2 for trend, p < 0.013). There was no correlation between type of system for sewage disposal and prevalence of infection (p = 0.8). In conclusion, the prevalence of H. pylori infection in Nossa Senhora do Livramento, a rural area from Brazil, is very high and similar to that observed in other developing countries. Furthermore, the increase in the prevalence of infection with age observed in this population seems to be due to both, cohort effect and acquisition of the infection during later life.

Adolescent↗
PubMedDate not supplied

[Furazolidone and cimetidine in patients with active duodenal ulcer and Helicobacter pylori in the gastric antrum].

In order to assess the effect of furazolidone on duodenal ulcer disease and Helicobacter pylori (Hp), a double-blind, double-dummy, randomized, prospective pilot study comparing furazolidone and cimetidine was carried out in 31 antral Hp positive patients with endoscopically proven active duodenal ulcer. Clinical, endoscopical, bacteriological and histological studies were done before and at four weeks of the study in all patients. If the ulcer was not healed, the treatment was extended for a further four weeks and all the procedures repeated. The healed ulcer patients were followed-up and re-endoscoped, regardless the presence of symptomatology, at least at 3 and 6mo after the end of treatment. It was observed that furazolidone cleared Hp (18% x 0%), healed the ulcer (91% x 87%) and reduced the relapse rate (30% x 92%; p < 0.025).

Adolescent↗