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Biomedical subjects

E N Smirnova

Publications and source records attributed to E N Smirnova.

At least 19 recordsLinked to original sources
PubMed1999

[Correlation between the duration of radiation adaptive response in bone marrow cells of mice and the dose of gamma-irradiation in vivo].

The dependence between the adaptive response and adaptive dose was studied on the basis of cytogenetic damage in polychromatic erythrocytes of bone marrow cells in mice after a low dose gamma-irradiation in vivo. The adaptive response to doses of 0.1 and 0.2 Gy was found to be retained for at least two months after irradiation. However, the adaptive dose of 0.4 Gy did not induce prolonged adaptive response.

Adaptation, Biological↗
PubMed1997

Action of chronic irradiation on the cytogenetic damage of human lymphocyte culture.

The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37 degrees C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation. All structural chromosome- and chromatid-type changes were recorded. The experimental data showed that the conditions of irradiation of lymphocytes affected neither the background level of chromosome damage nor their radiosensitivity. The obtained dose-response curve of chromosome aberrations was described by a linear regression, which then became a plateau. There is no statistically significant difference between the results for the low doses (10-50 cGy) of chronic and acute radiation.

Adult↗
PubMed1996

[Induction of cytogenetic damage in Chinese hamster cells after combined exposure to low doses of gamma-radiation and various chemical and physical agents].

Supersensitivity of Chinese hamster cells to low doses of gamma-radiation (dose range 5 to 50 cGy) was revealed by means of the cytokinesis-block micronucleus test. Treating these cells with caffeine (repair inhibitor) and mercaptoethylamine (radioprotector) and exposing them to secondary radiation emitted by protons with an energy of 70 GeV showed that this supersensitivity is associated with the absence of cytogenetic repair. When cells at the G2 phase received preliminary doses of 30 and 75 cGy before being irradiated at 150 cGy, the incidence of cytogenetic damage decreased, i.e., an adaptive cell response resulting from a radiation-induced mitotic delay was observed.

Adaptation, Physiological↗
PubMed1992

[The effect of hypoglycemic therapy on atherogenesis in diabetic patients].

Lipid blood fractions, malonic dialdehyde and antioxidant activity of plasma and red blood cells were evaluated in 56 patients with type I diabetes mellitus. Twenty-three of those had coronary heart disease. It was established that, if the patients received mean doses of insulin 80.5 +/- 2.70 U/day, they had higher levels of cholesterol, triglycerides and VLDL cholesterol than those who were treated with insulin in a dose of 43.2 +/- 1.84 U/day.

Adult↗
PubMed1990

[Optimal method of isolation of supra-epithelial mucous layer of the small intestine in experimental animals].

Experimental data on the activity of digestive endo- and exo-hydrolases in supra-epithelium mucus layer has been presented. Mucus layer has been obtained on special vibrating setting from the turned out intestinal preparation. Different conditions of work and medium of isolation have been used. The setting permits to obtain intestinal supra-epithelium mucus more completely and also prevents epithelium damages and hiting of damaged villi.

Animals↗
PubMedDate not supplied

[Induction of cytogenetic damages by combined action of heavy metal salts, chronic and acute gamma irradiation in bone marrow cells of mice and rats].

The aim of the present work was to study the combined action of salts of heavy metals (lead and cadmium), and acute and chronic gamma-irradiation on the cytogenetic damage to bone marrow cells of rats and mice. It was shown that the chronic exposure of rats and mice in vivo to gamma-irradiation induced the adaptive response. The salts of heavy metals supplemented to the diet of rats enhanced the cytogenetic damage to the non-irradiated animals, slightly enhanced the effect of chronic and acute gamma-irradiation, decreased the cytogenetic adaptive response induced by chronic gamma-irradiation.

Adaptation, Physiological↗
PubMedDate not supplied

[Detection of genomic instability in offspring of male mice chronically exposed to gamma-radiation by the "adaptive response" test].

The genomic instability (GI) in somatic cells of the progeny (F1 generation) of male mice chronically exposed to low-dose gamma-radiation was studied by comparative analysis of chromosome damage. BALB/C male mice exposed to 0.1 Gy (0.01 Gy/day) and 0.5 Gy (0.01 and 0.05 Gy/day) were mated with unirradiated females 15 days after irradiation. For comparison of radiosensitivity, two-month-old males, the descendants of irradiated and unirradiated animals, were subjected to irradiation with a dose of 1.5 Gy (0.47 Gy/min) from a 60Co source. GI was revealed by the standard scheme of adaptive response. The experiments indicated that, by using the test "adaptive response", it is possible to detect the transition of gamma-radiation-induced genomic instability in sex cells of male parent into somatic cells of mice (F1 generation) either from changes in radiosensitivity or by the absence of the adaptive response induced by a standard scheme.

Adaptation, Physiological↗
PubMedDate not supplied

[Low doses of radiation decrease the level of spontaneous and gamma-induced chromosomal mutagenesis in bone marrow cells of mice in vivo].

Low doses of ionizing radiation are known to induce adaptive response (AR), which is characterized in most cases by temporary nature, though the possibility of long-term persistence of AR is not ruled out. In this investigation we studied the effect of low doses of gamma-radiation on both high-dose radiation-induced and spontaneous level of cytogenetic damage throughout the life of mice. SHK male mice 2 months old were used. Priming doses of 0.1 and 0.2 Gy (0.125 Gy/min, gamma-radiation from 60Co) were used. A challenging dose of 1.5 Gy (1 Gy/min) was used in the experiments using a routine AR experimental design. The frequency of micronucleated polychromatic erythrocytes in bone marrow cells of primed, primed and challenged, and control groups was assessed at various times of animal life span. It was shown that: a) single low-dose gamma-irradiation induces a cytogenetic AR which can be revealed at 1, 3, 6, 9, 12 months after priming; b) single low-dose gamma-irradiation decreases the cytogenetic damage to a level below the spontaneous rate at the end of lifetime (20 months) of animals; c) ability to induce adaptive response does not depend on the age of animals at the moment of priming irradiation. In conclusion, the mechanisms underlying AR not only protect from chromosome damage induced by high-dose irradiation but also may play a role in spontaneous mutagenesis during aging of animals.

Adaptation, Physiological↗
PubMedDate not supplied

[The characteristics of the realization of cytogenetic damage in mammalian and plant cells exposed to low doses of radiation].

A complicated character of the cytogenetic injury dependence upon radiation dose was revealed after low-level gamma irradiation of Vicia faba seedlings and Chinese hamster fibroblasts. The dependence was linear with low-level secondary exposure to 70 GeV protons. The authors discuss a threshold nature of induction of the cytogenetic damage repair responsible for a high outcome of damages under the effect of low-level gamma radiation.

Animals↗
PubMedDate not supplied

[Results of early rehabilitation after various methods of cholecystectomy at Ust'-Kachka resort].

Early rehabilitation after open cholecystectomy or cholecystectomy from a mini-approach was performed in 101 patients with cholelithiasis treated surgically at Ust-Kachka health resort. Changes in clinico-laboratory indices reflecting the condition of hepatopancreatobiliary system and central hemodynamics before and after rehabilitation were studied depending on the variant of cholecystectomy. Advantages of early rehabilitation of patients after different variants of cholecystectomy at Ust-Kachka health resort in normalization of general condition of the patients and in prevention of functional disorders progression early after operation are demonstrated.

Balneology↗
PubMedDate not supplied

[Cytogenetic effect of gamma quanta and secondary radiation generated by 70 GeV protons on Chinese hamster fibroblasts].

The study of the cytogenetic effects (for instance, the chromosome aberration frequency in G2 cells and micronucleus formation) after exposure of Chinese hamster cells to gamma-rays and secondary radiation generated by 70 GeV protons showed that the relative biological effectiveness (RBE) of secondary radiation was approximately 3. The contribution of isochromatid deletions and exchanges into the total spectrum of rearrangements induced by the secondary radiation was different from that observed on gamma-irradiation. The absence of the modifying effect of caffeine on the cells exposed to the secondary radiation indicated that RBE of the secondary radiation from 70 GeV protons was mainly associated with its inhibitory action on the cytogenetic damages repair.

Animals↗