Biomedical subjects
Publications and source records attributed to E N Tverdokhlebov.
Principles of construction of devices for electrophoretical separation of mixes biopolymer molecules which length is from 5.10(4) to 10(7) base pairs have been briefly considered. Description has been made of main types of such devices, their parameters, merits and demerits. Data obtained by this method and possible perspectives of its development have been discussed.
Photoreduction of ferricytochrome P-450 with eosine radicals was studied by flash-photolysis. Reactivity of reduced protein was estimated by CO binding. It has been stated that both, at direct reduction of cytochrome P-450 and at its recombination with CO after its photodissociation of carboxycomplex the reaction consists of three stages with similar rate constants but different weight ratio of corresponding stages. This result well agrees with the supposition about non-equilibrium (in the course of reduction) distribution of cytochrome P-450 conformers.
Kinetics of cytochrome P-450 reduction with methylviologen radicals was investigated by flash-photolysis. Formation of carboxycomplex of reduced protein and effect of temperature and substrates of the I type on this reaction were studied. It has been shown that CO addition to protein proceeds in two stages. With temperature increase (20-45 degrees C) and when benzphetamine or hexabarbital are added to solution the weight of the first (faster) stage increases. Such regularity is easily explained by the presence in solution of two conformers of oxidized cytochrome P-450 (with different reactivity) whose equilibrium depends on temperature and presence of the substrate.
Reaction ability of partially reduced human methemoglobin was estimated by the rate of protein addition of carbon monoxide immediately after reduction. Impulse of photoexcitation allowed two variants of these experiments to be carried out: immediately after partial reduction of methemoglobin with photoradicals of benzophenone 3,3',4,4'-tetracarbon acid and after photodissociation of the carboxy-complex obtained in the first case. It has been shown that at protein reduction "p" less than or equal to 0.03 under these conditions the initially obtained R-form of protein is partially transformed into the less reactive T-form.
This report reviews the activities of the "Reagents, Devices, Probes" branch of the Russian State "Human Genome" Project for six-year period (1989-1994). Data on pilot and commercial production of reagents and equipment for human genome studies along with information on the project costs and awarded grants are presented.
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