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Biomedical subjects

E Nugel

Publications and source records attributed to E Nugel.

31 records · Page 2Linked to original sources
PubMed1982

Quantitation of a pregnancy associated globulin in the rat by two-site binding enzyme immunoassay.

An indirect two-site binding enzyme immunoassay has been developed to quantify the pregnancy associated globulin (PAG) in rats. Anti-PAG from guinea pig is adsorbed to solid phase and binds serum-PAG, which then reacts with its remaining free determinants with anti-PAG from rabbit. Sheep anti-rabbit IgG, labeled with horse radish peroxidase (HRP), binds to the rabbit anti-PAG antibody and the enzymic activity of the solid phase is measured by incubation with the appropriate chromogenic substrate. The optical density is directly related to the quantity of PAG to be measured. Reproducible results are obtained in the range from 5 to 200 micrograms/l. The test detects 12 fmoles of PAG. Female rats show a 1000-fold higher serum level of PAG than male rats. Strain differences of the distribution of the PAG serum concentration were only found between male rats.

Animals↗
PubMed1982

Abnormalities in the composition of serum high density lipoprotein in renal transplant recipients.

Levels of HDL-cholesterol, HDL-triglyceride and apolipoprotein A were determined in the sera of 26 female and 44 male renal transplant recipients. In comparison with controls, no significant abnormalities in HDL-cholesterol values could be found, but apolipoprotein A and HDL-triglyceride concentrations, were increased significantly. The increased apolipoprotein A/HDL-cholesterol ratio and the decreased apolipoprotein A/HDL-triglyceride and HDL-cholesterol/HDL-triglyceride ratios in these patients demonstrate abnormalities in HDL-composition. These results permit the conclusion that, in renal transplant recipients, the levels of HDL-cholesterol and particularly the changes in HDL-composition should be taken into account when considering the reason for the high incidence of cardiovascular disease in these patients.

Adult↗
PubMed1981

Temperature dependent rise in activity of horseradish peroxidase caused by non-ionic detergents and its use in enzyme-immunoassay.

Non-ionic detergents such as polyoxyethylene-octylphenol or -sorbitolester were found to increase activity of horseradish peroxidase due to delay of inactivation in the course of substrate reaction. This rise in activity was investigated using different chromogens and was highest with o-dianisidine. An increasing stability of the enzyme to higher reaction temperatures was observed when detergents were added to the substrate solution, and the action of detergents also is enhanced with increasing reaction temperature and time. Different degrees of activation were found when comparing substrate conversion with and without detergents using free peroxidase (2.7-fold) and conjugated peroxidase bound to the solid phase by antigen antibody reaction (1.8-fold). In enzyme-immunoassay, detection limit and analytical sensitivity can be doubled.

Antigen-Antibody Reactions↗
PubMed1981

Comparison of chromogens for the determination of horseradish peroxidase as a marker in enzyme immunoassay.

o-Phenylenediamine, 2,2'-azino-di(3-ethylbenzthiazoline sulphonic acid-6) (ABTS), o-dianisidine and 4-aminoantipyrine were compared as chromogens for the determination of horseradish peroxidase. Highest sensitivity in the determination of horseradish peroxidase-IgG conjugates in dissolved form was obtained with o-phenylenediamine. When these conjugates were used in a two-site binding enzyme immunoassay for hepatitis B surface antigen (HBsAg), the steepest calibration curve and the lowest detection limit were obtained when ABTS was used to determine the immune complexes bound to the solid phase. Non-ionic detergents, such as polyoxyethylene-sorbitol ester, retarded horseradish peroxidase inactivation, resulting in a chromogen-dependent activity rise of horseradish peroxidase. An optimised determination of horseradish peroxidase is reported, in which the sensitivity of the solid phase enzyme immunoassay is doubled by the use of o-dianisidine.

Ampyrone↗
PubMed1981

Effect of IgG-horseradish peroxidase conjugates purified on Con A-Sepharose upon sensitivity of enzyme immunoassay.

Antibodies coupled to horseradish peroxidase (HRP) were purified by gel filtration and by combination of affinity chromatography and gel filtration. Binding kinetics to solid phase bound antigens were studied of the antibody-enzyme conjugates in the presence and in the absence of unlabelled IgG. The detection limit in enzyme immunoassay was found to be lowered by factor 16 after elimination of unlabelled IgG from the conjugate.

Animals↗
PubMed1980

Increased creatine kinase BB activity in rat plasma induced by hypoxia.

Rats show an elevated creatine kinase (CK) activity in plasma after a 20-hour exposure to hypoxia. The increase of CK activity is essentially caused by CK-BB activity attaining its maximum at the 3rd day after exposure to hypoxia. The possibility of establishing a potential brain damage by determining CK-BB activity in plasma is discussed.

Animals↗
PubMed1980

Lecithin: cholesterol acyltransferase activity, HDL-cholesterol and apolipoprotein A in serum of patients undergoing chronic haemodialysis.

In comparison with a control group, significantly lower lecithin:cholesterol acyltransferase activities and HDL-cholesterol values were found in the serum of patients undergoing chronic haemodialysis, while apolipoprotein A concentration remained unchanged. Reduced activity of lecithin:cholesterol acyltransferase is an indication of the abnormal cholesterol metabolism in haemodialysed patients and could be, in addition to other factors, an explanation for the high incidence of cardiovascular diseases in this patient group.

Apolipoproteins↗
PubMed1979

Creatine kinase isoenzyme BB in serum of healthy adults and children.

The activity of the creatine kinase isoenzyme BB was determined in the serum of 26 healthy adults and 31 children. The isoenzyme BB could be proved as a normal component in the human serum. In the adults examined, an activity of 0.56 +/- 0.16 I.U./l (x +/- S.D.) was determined. The activity of creatine kinase isoenzyme BB in the serum does not depend on sex but is subject, however, to a strong age dependence. Only at an age of more than 18 years, isoenzyme BB activities adjust to those of adults.

Adult↗
PubMed1976

[Thromboelastography and inhibitors of aggregation].

The effect of maintained treatment with salicylo-acetic acid (1.5 g/day) on the thrombelastogram was examined in the venous blood from 18 accidental patients. In the prevention of thrombembolia, resulting values of the thrombelastograph are not sufficient to show functional changes of platelets. Some of the patients revealed prolonged times for reaction and formation of coagula, thus discreet influencing the thrombelastogram might be supposed.

Adult↗