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Biomedical subjects

E Ouchi

Publications and source records attributed to E Ouchi.

At least 37 records · Page 2Linked to original sources
PubMed1980

HLA antigens in juvenile onset diabetes.

To study association between juvenile onset diabetes (JOD) and major histocompatibility gene complex, 40 patients with childhood onset diabetes and 120 healthy subjects were typed for HLA. Bw54 was present in 33 percent of the patients with JOD, while it appeared in 8 percent of the controls. Expressed as a relative risk, the antigen Bw54 confers a susceptibility to the development of JOD which is 5.3 times that in the controls. JOD shows a little high degree of association with A9 (78%). However, the A9-antigen is common in the Japanese and appears in 58 percent. Though less striking, the decreased frequency of B12 was 3 percent of JOD, less than 15 percent of the controls (p less than 0.05). There was no association between Bw54 and JOD with family history of diabetes.

Adolescent↗
PubMed1978

Effect of amphotericin B on the incorporation of neocarzinostatin into human gastric cancerous tissues.

AmB/NCS combination therapy against cancer was evaluated. Seven gastric cancer and 2 gastric polyp cases were treated in this manner and in addition 2 gastric cancer patients were treated with NCS alone, to serve as the control. AmB dissolved in glucose solution was administered intravenously as was NCS, which was given at the onset of gastric surgery. Lesion tissues and healthy tissues were collected from each patient and the NCS titers measured by bioassay. It was shown in the majority of the gastric cancer cases that the NCS levels in the lesion tissues were substantially higher than in the surrounding normal tissues, whereas in the gastric polyp and the gastric cancer patients who received NCS alone, no significant differences were found between the tissues.

Adult↗
PubMed1976

Clinical significance of Congo red test.

Clinical significance of Congo red test was studied, especially on its relation to the liver function and amyloidosis. No significant relationship was observed between Congo red test and Indocyanin green test in cases of various liver diseases except liver cirrhosis. Histological studies also revealed that there were no pathologic features specifically affecting Congo red index (CRI), even though CRI in cases of various liver diseases tended to be increased as compared with other diseases. As a cause of increased CRI which was seen most remarkably in cases of liver cirrhosis, obstructive change of the liver blood flow followed by the hepatic tissue damage is suspected. The concept that Congo red test is a kind of the hepatic excretory function test does not have, from our data, enough evidence. The present study confirmed our previous data on the fate of Congo red injected intravenously, which indicated that this test is one of the best tests available for RES function and this test is related mainly to Kupffer's cell rather than to the so-called hepatic excretory function. There were no cases which showed notable decrease of CRI in experimental amyloidosis and in clinical amyloidosis, and it can be said that Congo red test is not the best test for the diagnosis of amyloidosis.

Acute Disease↗
PubMed1976

Erythrocyte rosette forming activity of macrophage.

Erythrocyte rosette forming activities of the rat lung macrophage, peritoneal macrophage, Kupffer cells and expressed splenic cells were studied. The erythrocytes employed were fresh human red blood cell (RBC), fresh sheep RBC (SRBC), and tanned SRBC coated with bovine albumin or with bovine gamma-globulin. The macrophages were mixed with RBC at a ratio of 1 to 100 or 200 in gelatin veronal buffer or phosphate buffered saline. The experiments were carried out at 4 degrees C. No autologous serum nor anti-RBC serum was added to the experimental system. 10 to 20 per cent of the lung macrophages formed rosette and their entire surfaces were covered with RBC. The peritoneal macrophage and Kupffer cells were also capable of forming rosette with fresh SRBC as well as with SRBC coated with bovine albumin or with bovine gamma-globulin. Examinations for cell surface immunoglobulin of alveolar macrophage, peritoneal macrophage, Kupffer cells and splenic cells revealed that alveolar and peritoneal macrophages apparently possessed surface gamma-globulin and that almost all of these macrophages possessed surface immuno-globulin. In order to examine the effect of different natures of SRBC on in vivo phagocytosis, rats were immunized with SRBC by injecting various SRBC into the femoral or the portal vein and the hemagglutinin titer was studied. The maximum hemagglutinin titer was observed in the rat injected with fresh SRBC into the femoral vein and the minimum hemagglutinin titer was observed in the splenectomized rat injected with fresh SRBC and in the rat injected with SRBC coated with albumin or gamma-globulin into the femoral or portal vein. Immunological role of Kupffer cells was discussed.

Animals↗
PubMed1975

Reticuloendothelial system function test using 131I-labeled aggregated albumin.

The reticuloendothelial system function test using 131I-labeled aggregated human albumin (131IAA) as test particle is thought to be one of the best methods for the study of RES function of human beings. This method needs a large amount of 131IAA, but 131IAA has been used as a tracer of liver scintiscanning, and we tired to study RES function by using commercially available 131IAA specially made as tracer of liver scintiscanning. Because the amount of 131IAA used in this study was smaller than the critical dosis, our interest was focussed on the catabolic activity of 131IAA phagocytized by Kupffer's cells. The plasma obtained 60 min after 131IAA injection was fractionated into two distinct peaks by gel column chromatography; ionic 131I and protein-bound 131IAA. The ratio between ionic 131I and bound 131IAA (F/B ratio) was studied in various diseases. The F/B ratios of liver cirrhosis and hepatoma were significantly lower than that of the control. The lowered F/B ratio had no correlation with other liver function tests, data of hepatic scintiscanning, or with clinical findings. The F/B ratio was also not correlated with congo red index as RES function test.

Adult↗
PubMed1975

Lympho-reticuloendothelial activity of early gastric carcinoma.

The lympho-reticuloendothelial activities of 54 cases of early gastric carcinoma were studied. Morphological blastoid transformation of lymphocyte by the addition of phytohemagglutinin-P (PHA-P) was disturbed in half of the patients. The DNA synthesizing activity of lymphocyte was not stimulated by PHA-P in the cases of advanced carcinoma but it was stimulated in the cases of early carcinoma. The tuberculin reaction was negative in 30% of the cases of early carcinoma. The reticuloendothelial system (RES) activity studied through the congo red method revealed that 86% of the cases of early carcinoma had disturbed RES function.

Adolescent↗