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Biomedical subjects

E P Berg

Publications and source records attributed to E P Berg.

10 recordsLinked to original sources

Pork carcass composition derived from a neural network model of electromagnetic scans.

We used an advanced computer logic system (NETS 3.0) to decipher electromagnetic (EM) scans in lieu of traditional linear regression for estimation of pork carcass composition. Fifty EM scans of pork carcasses were obtained on-line (prerigor) at a swine slaughter facility. Right sides were cut into wholesale parts and dissected into fat, lean, and bone to obtain total dissected carcass and primal cut lean. In this study, the input layer consisted of 81 nodes (80-point EM scan curve and warm carcass weight), one hidden layer of 42 nodes, and an output layer consisting of one node, which were run separately for outputs of ham, loin, or shoulder lean. The hidden layer connected to the output of total lean contained 50 nodes. Thirty-five scans were used for training of the network. The new network was then tested with 15 previously unseen input/output pairs. Separate neural networks were developed for the estimation of dissected total carcass, ham, loin, and shoulder lean. The NETS configuration improved on linear regression equations for estimation of total carcass lean by .31 kg, ham lean by .284 kg, and shoulder lean by .148 kg. Our results show that advanced computer logic systems have the capacity to improve upon traditional linear regression equations for prediction of pork carcass composition.

Animals

Evaluation of electronic technology to assess lamb carcass composition.

Accurate price signals are essential for producers of American lamb to ensure production of uniformly lean animals. Development of carcass merit-pricing systems will require the use of objective technology for assessing carcass composition or lean distribution. The objective of this study was to evaluate electronic technologies for accurate determination of lamb carcass composition. Lambs (n = 106) were selected as a representation of U.S. market lambs that transcended geographic location, sex, breed, carcass weight, yield grade, and production system. The independent variables used to predict lamb composition varied with the technology. The electronic technologies tested included realtime ultrasound, optical reflectance probe, bioelectrical impedance analysis, and electromagnetic scanning (TOBEC). All technologies, except realtime ultrasound, were tested on warm (prerigor) carcasses and repeated after a 24-h chill. Longitudinal ultrasonic scans of fat and muscle tissue depth and grading probe fat depths were marginal predictors of proportional carcass yield. The TOBEC measurements often accounted for more variability associated with kilograms of dissected lean and percentage of carcass lean than did carcass weight. Equations from TOBEC measurements were the most accurate predictors of weight and percentage of dissected and fat-free lean. Bioelectrical impedance measurements of resistance and reactance combined with carcass weight were also good predictors of carcass composition. Prediction of carcass lean distribution by measures of TOBEC were the most accurate for prediction of leg lean. The implications of usefulness of these technologies will depend on the commitment of the U. S. sheep industry in development of a lamb price discovery system based on carcass composition.

Animals

Assessment of lamb carcass composition from live animal measurement of bioelectrical impedance or ultrasonic tissue depths.

Market weight lambs, average weight 52.5 kg (+/-6.1), were used to evaluate nontraditional live animal measurements as predictors of carcass composition. The sample population (n = 106) represented U.S. market lambs and transcended geographic location, breed, carcass weight, yield grade, and production system. Realtime ultrasonic (RU) measurements and bioelectrical impedance analysis (BIA) were used for development and evaluation of prediction equations for % boneless, closely trimmed primal cuts (BCTPC), weight or % of dissected lean tissue (TDL), and chemically derived weight or % fat-free lean (FFL). Longitudinal ultrasonic images were obtained parallel to the longissimus thoracis et lumborum (LTL), positioning the last costae in the center of the transducer head. Images were saved and fat and LTL depths were derived from printed images of the ultrasonic scans. Bioelectrical impedance analysis was administered via a four-terminal impedance plethysmograph operating at 800 microA at 50 kHz. Impedance measurements of whole-body resistance and reactance were recorded. Prediction equations including common linear measurements of live weight, heart girth, hindsaddle length, and shoulder height were also evaluated. All measurements were taken just before slaughter. Bioelectrical impedance measurements (as compared to RU and linear measurements) provided equations for %BCTPC, TDL, %TDL, FFL and %FFL with the highest R2 and lowest root mean square error. Even though BIA provided the best equations of the three methodologies tested, prediction of proportional yield (%BCTPC, %TDL, and %FFL) was marginal (R2 = .296, .551, and .551, respectively). Equations combining BIA, RU, and linear measurements greatly improved equations for prediction of proportional lean yield.

Animals

Alternative pork carcass evaluation techniques: I. Differences in predictions of value.

Dissected and predicted wholesale and lean boneless values for 154 pork carcasses representing seven genotypes with substantial variation in carcass composition and percentage of lean were determined. Dissected carcass value was determined using a component pricing model, and four alternative models were specified to predict that value. The models included measurements from a ruler (RULER) and two carcass evaluation technologies, Hennessy probe (PROBE) and electromagnetic scanner (EMS1). A combination of the PROBE and EMS1 models (EMS2) was also used. For wholesale value, R2 were .40, .70, .59, and .74, and the RSD were 8.18, 5.77, 6.76, and 5.38 ($/100 kg of carcass value) for RULER, PROBE, EMS1, and EMS2, respectively. For lean boneless value, the R2 were .41, .73, .59, and .74, and the RSD were 8.34, 5.67, 6.99, and 5.51 ($/100 kg of carcass value) for RULER, PROBE, EMS1, and EMS2, respectively. The results indicate that a combination of probe and electromagnetic scanner measurements provided the best fit to dissected value.

Animals

Alternative pork carcass evaluation techniques: II. Statistical analysis of error attributable to sex, genotype, and weight.

Carcasses of 154 hogs representing seven genotypes with substantial variation in carcass composition and percentage of lean were completely dissected and analyzed. Measurements from a ruler, Hennessy probe, and electromagnetic scanner were each used to predict wholesale and lean boneless carcass value. Error, defined as dissected value minus predicted value, due to the omission of sex, genotype, weight, and their interactions was estimated for each model. The errors were significantly different from zero for the models using ruler and electromagnetic scanning measurements separately (P < .01). Errors due to sex, genotype, weight, and their interactions were greatest for the less lean barrows. A combination of probe and electromagnetic scanner measurements resulted in the least error. The value of barrows with low percentage of lean was consistently overpredicted, whereas the value of leaner gilts was underpredicted for the models using ruler and electromagnetic scanning separately (P < .001).

Analysis of Variance

Electromagnetic scanning of pork carcasses in an on-line industrial configuration.

The objective of this study was to test and validate electromagnetic scanning of whole pork carcasses in an on-line, integrated, industrial configuration. The electromagnetic (EM) scanner was installed in two pork processing facilities (Plant A and Plant B). Plant A was a small pork fabrication plant that further processed chilled pork carcasses. Carcasses were delivered to Plant A by refrigerated trucks. The amount of EM energy absorbed by the carcasses was recorded as they were conveyed through the EM field. A plot of the absorption units over time (EM scan curve) was used to obtain predictive variables for estimating carcass and primal cut composition. Forty-eight whole, chilled carcasses (Group A) were electromagnetically scanned and conveyed onto the fabrication line. The average percentage carcass lean for Group A was 49.1% (range = 36.5 to 59.5%). Right carcass sides were removed from the processing line, fabricated into primal cuts, and dissected into fat, lean, and bone. Prediction equations were developed from EM scans for weight of total dissected carcass lean (R2 = .830; root mean square error = 1.80 kg), percentage of carcass lean (R2 = .820; root mean square error = 2.29%), and weight of dissected ham, longissimus muscle, and shoulder lean. In Plant B, the electromagnetic scanner was installed at the end of a pork slaughter line to ensure carcass scanning at a consistent carcass temperature. Fifty whole, pre-rigor eviscerated carcasses (Group B) were electromagnetically scanned before entering the chill cooler where fat and loin tissue depths were obtained by an optical grading probe. The average percentage carcass lean for Group B was 46.7% (range = 30.1 to 57.3%). Prediction equations were developed from EM scans for weight of total dissected carcass lean (R2 = .904; root mean square error = 1.59 kg), percentage of carcass lean (R2 = .863; root mean square error = 2.05%), and weight of dissected ham, loin, and shoulder lean. Statistical equations developed for the prediction of dissected primal cut lean were superior from EM scans of Group B (prerigor) carcasses. Electromagnetic scanning proved more statistically efficient than optical probes for predicting weight of dissected carcass lean and percentage of carcass lean. Statistical comparison of EM scan equations from Groups A and B are not completely valid because two different populations of carcasses were tested at different times of the year. The results of this study show that EM scanning has the potential to accurately predict pork carcass composition in a fully automated, on-line industrial configuration.

Animals

Bioelectrical impedance analysis for the prediction of fat-free mass in lambs and lamb carcasses.

Ninety-eight commercial crossbred lambs, average weight 55.1 kg (+/- 4.95), were used to evaluate bioelectrical impedance analysis (BIA) as a prediction method for fat-free tissue from live lambs and lamb carcasses. Lambs were transported to the abattoir, restricted from feed for 10 h, weighed (LWt), and measured for body resistance (Rs, ohms), body reactance (Xc, ohms), and distance between detector terminals (L, cm). Following slaughter, hot carcasses were weighed (HCWt; average 31.4 +/- 2.8 kg) and impedance measurements of Rs, Xc, and L were recorded from readings on the dorsal and lateral sides of the carcasses. Temperatures (average = 39.2 degrees C) were recorded. Carcasses were chilled for 24 h. Cold carcass weights (CWt; average 31.1 +/- 2.8 kg) and temperatures (average = 1.0 degrees C) were recorded and BIA measurements were repeated. Carcasses were split down the midline and right sides were ground three times and sampled for chemical analysis of fat, moisture, ash, and protein. Fat-free mass (FFM) was calculated as [Wt-(Wt x %Fat)]. Fat-free soft tissue (FFST) was calculated as the sum of carcass chemical protein and carcass water. Regression equations predicting fat-free tissue for the live lambs (FFMlive and FFSTlive), hot carcasses (FFMhot and FFSThot), and cold carcasses (FFMcold and FFSTcold) were developed. The independent variables LWt, Rs, and Xc accounted for 77.7% (residual mean square error [RMSE] = 1.97 kg) of the variation in FFMlive and 78.6% (RMSE = 1.78 kg) of the variability of FFSTlive.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

Electromagnetic scanning to predict lamb carcass composition.

The electromagnetic scanner generates a constant, low-level electromagnetic field (2.5 MHz) within a large plexiglass tube. The amount of electromagnetic (EM) energy transferred (to the carcass) is highly related to lean tissue. A plot of the absorption units over distance can be used to assess the total mass of lean tissue and of the respective primal cuts. The difference in curve height between two points (D), peak phase absorption, and linear carcass measurements (pre-rigor, HCWT or post-rigor, CWT carcass weight, and carcass length, LENG) were used to predict total dissected lean (TOTLEAN), dissected leg lean (LEGLEAN), and percentage of dissected carcass lean (PERLEAN). Twenty-one pre-rigor and 22 post-rigor (24 h chill) lamb carcasses, average weight 26.8 (+/- 4.2 kg) and 26.4 (+/- 4.1 kg) kg, respectively, were evaluated from measurements of total body electrical conductivity (TOBEC). Two geometric orientations were tested for statistical accuracy in this study: A) each carcass entered the EM tunnel rear leg first, on its left lateral side, neck facing the right side of the tunnel; and B) each carcass entered the EM tunnel rear leg first, breast down, and neck up. Orientation A proved more statistically efficient for pre-rigor carcasses, and orientation B was more desirable for post-rigor carcasses. Multiple-regression models involving HCWT, LENG, and a single D measure accounted for 98.0 and 95.0%, respectively, of the total variation in pre-rigor carcass TOTLEAN and LEGLEAN in A.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals

Faith healing.

This article has attempted to show the relevance of faith and prayer to the healing arts. Whilst its frame of reference differs from that of the medical scientist, its application is not incompatible with conventional medical practice. It springs from a belief that all healing ultimately resides with God, who acts through divinely appointed laws to energize the natural healing processes of the body. Through faith and prayer, attunement is possible with God resulting in an energy flow to the patient. This belief is supported by scientific evidence. Faith healing supports the concept of 'healing of the whole person', and challenges the medical scientist and practitioner to develop a greater awareness of the 'unknown factors' working in recoveries of patients beyond normal medical expectation.

Aged

Stereochemical studies on medicinal agents. 21. Investigation of the role of conformational factors in the action of diphenylpropylamines. Synthesis and analgetic potency of 5-methylmethadone diastereomers.

The synthesis of racemic threo- and erythro-5-methylmethadone (3a and 3b, respectively) was carried out and the solution conformation of each isomer was investigated through pKa and NMR studies. The data indicate that 3a-HCl exists exclusively in an internally hydrogen-bonded conformation while the erythro isomer 3b-HCl is present as a mixture of conformations. The erythro racemate 3b was found to possess 5.4 times the analgetic potency of (+/-)-methadone in contrast to the threo racemate 3a which was inactive and devoid of antagonist activity. The fact that the inactive racemate 3a contains the 5S,6R stereoisomer, which combines the configurations found in the more active enantiomers of methadone and isomethadone, suggests that the chiral centers do not behave as independent units and that conformational factors are playing an important role in governing stereoselectivity. These results, when analyzed together with earlier reports, suggest that one of the pharmacophoric conformations of the diphenylpropylamine analgetics possesses an antiperiplanar-like disposition of the Ph2CCOEt and +NHMe2 groups.

Analgesics