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Biomedical subjects

E Panek

Publications and source records attributed to E Panek.

At least 19 recordsLinked to original sources
PubMed1992

[Prostaglandins PGF2alpha in the fight against bleeding and hemorrhage the uterus during the climacteric period].

This article is about usage prostaglandins to fight against bleeding and haemorrhage from uterus in climacteric period. Prostaglandins are used widely by gynecologist and obstetrics as most powerful contracting drugs, namely in this situations when we observe returning, plentiful bleeding from uterus. Women with climacteric bleeding had administer prostaglandins intravenous or immediate intra-cervix. They are very good drug, strongly and a long time hold up bleeding. This method give us time to better preparing patients to operation and diminish losses of blood. Side effects are small and not trouble. Prostaglandins are very simply in usage and characterise very good haemostatic effect, recognised faster then in typical methods like hormonal, mechanical antifibrinolytic etc.

Climacteric↗
PubMed1990

Conjugational cotransfer of IncFI and IncI conjugative plasmids forming aggregate in a pathogenic Citrobacter freundii strain.

Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotransfer of both plasmids is observed--an unusually high fraction of transconjugants acquiring pEM6 acquires pEM4 and vice versa. The observed cotransfer of these plasmids is connected with their ability to complement their conjugational functions. An insertion mutant of pEM4 with decreased frequency of conjugational transfer has been isolated. This mutant (pEM44) lost its ability to mobilize nonconjugative plasmids. Its transfer is stimulated by pEM6 and it is transferred from (pEM44, pEM6) donors almost exclusively with pEM6 plasmid. The role of cotransfer and stimulation phenomena in spreading of plasmid aggregates in bacterial population is discussed.

Bacteriocin Plasmids↗
PubMed1989

Mobilization of R387 Inc K conjugative plasmid by the conjugative plasmid R64 Inc I.

Plasmid aggregate (R387, R64) was constructed in E. coli K12 strain. Plasmid R387 Inc K was stimulated to conjugational transfer by plasmid R64 Inc I. This stimulation was caused neither by recombination between both plasmids nor by trans-complementation of R387 conjugational systems by gene(s) product(s) of R64 plasmid. The observed phenomenon resembled rather mobilization of nonconjugative plasmids by conjugative ones. As in mobilization, the observed increase in R387 transfer frequency could take place only when both interacting plasmids were present in donor cells. Moreover, the entry exclusion system functioning in recipient cells, toward stimulating R64 plasmid affected strongly the conjugational transfer of stimulated R387 plasmid. Analogous phenomenon was observed during mobilization of nonconjugative plasmids by conjugative ones.

Citrobacter↗
PubMed1989

Genetic properties of plasmids isolated from pathogenic strain of Citrobacter freundii.

It was found that Citrobacter freundii SLJ10 strain was an etiological factor of swine diarrhoea in North Poland. This strain harboured a plasmid aggregate consisting of two plasmids-pEM4 responsible for virulence and pEM6 for selective advantage of the strain, determining resistance to tetracycline and production of bacteriocin. These two plasmids cooperated in conjugal transfer and formed an infective block of genetic information, which could be transferred to other strains of Enterobacteriaceae and cause their virulence.

Citrobacter↗
PubMed1985

[Immunoelectrophoretic data and serum immunoglobulin G, A and M levels in workers engaged in the production of organophosphate pesticides].

Immunoelectrophoresis of serum proteins and concentrations of IgG, IgA and IgM in serum were estimated in 83 workers employed at the production of organic phosphorus insecticides. In 56.6% of immunoelectrophorograms deviations from normal were found, most frequently a rise of acute phase proteins and IgG. Quantitative determinations showed a rise of serum IgG and IgA and a fall of IgM concentrations as compared to a control group of nonexposed persons.

Adult↗
PubMed1983

[Electrocardiographic examination of the heart and the study of catecholamine secretion in workers subjected to mechanical vibration and occupational noise].

Electrocardiographic tests in 281 workers of metallurgical industry, exposed for 3-20 years to mechanical and acoustic vibrations exceeding allowable standards, were carried out. The control group consisted of 120 subjects unexposed to physical or chemical hazards. In 82 subjects exposed to vibration and noise, 43 workers exposed to isolated noise and 30 subjects of the control group, the amount of 3 methoxy--4 hydroxymandelic (3M4HM) acid before and after work was determined. Significantly more frequent repolarization disturbances of neurovegetative nature (V-3), particularly in those simultaneously exposed to general and local vibration, were evidenced. In those exposed exclusively to noise no ECG changes were found. In addition, increased 3M4HM secretion, due to both local and whole--body transmitted vibration, was found. Isolated noise does not induce an increase in 3M4HM secretion.

Adult↗
PubMed1981

Biological variations in hyperlipidemic children and adolescents treated with fenofibrate.

Beside their pharmacological action drugs may have unexpected side effects which must be known and taken into account in the interpretation of laboratory tests. In this work, we followed 17 hyperlipidemic subjects, aged 4-19 years, treated with fenofibrate only. We performed several plasma tests before and during treatment. The hypolipidemic action of fenofibrate was evident: decreases of cholesterol and triglycerides mean values were, respectively, 22% and 39% after 3 months of treatment. Other plasma constituents were also significantly decreased: uric acid, 20%; bilirubin, 19%; alkaline phosphatases, 15%. The aminotransferase activities (both AST and ALT) were increased in only four subjects after 3 months. The highest values were 88 U/l for AST and 109 U/l for ALT (the initial values were, respectively, 28 U/l and 23 U/l). These activities decreased when the treatment was stopped. These variations were isolated and transient.

Adolescent↗
PubMed1980

Personal and familial factors in cholesterolemia: criteria for selection of a reference population.

We studied factors affecting cholesterolemia in a presumably healthy population of 23 300 subjects, ages 20 to 59 years. Multiple-contingency tables were established, to quantify the information obtained by means of a factorial correspondence analysis. The relations between values for cholesterol and those for triglycerides, uric acid, and overweight were constant for all ages and both sexes. Cholesterolemia paralleled alcohol and tobacco consumption and arterial blood pressure. Use of oral contraceptives did not influence cholesterol values. We classified variation factors according to the statistical method of segmentation, by sex and age. This classification permitted us to establish criteria for excluding subjects from a population sampled with the aim of producing reference values for plasma cholesterol. The values of cholesterol for the 50th percentile were 5.40 mmol/L for men 20 to 39 years old and 5.79 mmol/L for those older than 40. For women, the 50th percentile values increased from 5.34 mmol/L between ages 20 to 39 to 5.92 mmol/L for ages 40 to 49 and to 6.51 mmol/L after 50.

Adult↗
PubMed1979

The effect of sex, deviation from ideal weight and sampling time on blood constituents in presumably healthy subjects.

In order to verify the influence of sampling time on blood constituents, populations of supposedly healthy subjects were grouped according to age, sex, deviation from their ideal weight, state of fasting or nonfasting, and time of sampling. Each fasting subject in one group underwent two samplings during the course of a morning: the first at 08.00 and the second between 09.00 and 12.00. In the second group, the first was taken at 13.00, and the second between 14.00 and 16.00. Subjects in the second group had eaten a standard meal of 700 calories at 12.00. Differences between the paired samples from a given individual are discussed with respect to the time of sampling for plasma urea, creatinine, proteins, albumin, calcium, sodium, potassium, cholesterol, uric acid, chloride ions, phosphate, bilirubin, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine phosphokinase, alkaline phosphatase, hemoglobin and erythrocyte and leukocyte counts. Variations due to the time of sampling were large for phosphorus, bilirubin, and leukocyte count.

Adult↗
PubMed1979

Enzymic assay of plasma cholesterol: a comparison of analytical variations found using the Greiner GSA II and the Technicon SMA 12/60 and SMA II.

Cholesterol was assayed on the Greiner GSA II and the Technicon SMA 12/60 and SMA II autoanalyzers, with Allain's entirely enzymic method (Allain et al. (1974), Clin. Chem. 20, 470--475). The coefficients of variation in the results over a period of one month were less than 6% with the GSA II and the SMA II, but up to 9% on the SMA 12/60. Although the accuracy of the enzymic method depends on the specificity of the cholesterol oxidase and cholesterol esterase, the results were in good agreement with those obtained by Abell's method (Abell et al. (1952), J. Biol. Chem. 195, 357--366) on fresh or freeze dried human plasma. The cholesterol concentration was stable at 4 degrees C, or in frozen or freeze dried plasma. None of the drugs tested interfered with the enzymic assay.

Autoanalysis↗
PubMed1979

Factors affecting the concentration of triacylglycerols (triglycerides in plasma: reference values for adults.

We studied factors influencing concentrations of triglycerides in the blood of a presumably healthy population of about 9000 persons. Using multi-dimensional analysis, we show, in addition to various related to sex and age, the relation between triglycerides and uric acid, overweight, arterial blood pressure, use of oral contraceptives (in women), consumption of alcohol and tobacco, and lack of physical exercise. These factors were classified as a function of their discriminative power by the statistical method of segmentation. We thus chose the following factors as criteria for exclusion from a reference population for the values of triglycerides in blood: in men 20 to 39 years old, overweight more than 90%; in men 40 to 59 years old, overweight of more than 115%, consumption of more than half a liter of wine per day or of more than 11 cigarettes, use of medicines, and a blood uric acid exceeding 420 mumol/L; in women 20 to 39 years old, contraceptives and overweight; in women 40 to 59 years old, overweight or more than 130% and the intake of medicines. At the 97.5th percentile of the reference population as compared with a non-selected population, the values were 80% less for men 20 to 39 years old, 95% less for men 40 to 59 years old, and only 26 to 30% less for women 20 to 59 years old.

Adult↗
PubMed1978

Analytical interferences of drugs in clinical chemistry.

The effects of several drugs were tested on analytical methods used in the clinical laboratory to measure the concentration of certain chemical constituents and activity of enzymes in body fluids. Drugs were studied initially at reported toxic concentrations, but if they had an effect at this level effects of lower concentrations were also investigated. The drugs were first studied on the methods of the Technicon SMACTM analyzer. Subsequently, methods in use with the Du Pont ACA and Technicon AutoAnalyzerTM I or II were evaluated. The most commonly affected method was the phosphotungstate reduction procedure for measuring uric acid. However, methods for measuring total protein and albumin were also affected frequently, as were determinations of alkaline phosphatase and lactate dehydrogenase activity.

Chemistry, Clinical↗
PubMed1978

[Adaptation of an entirely enzymatic triglyceride assay to the GSA II Greiner. Application to the study of the stability of triglycerides, and factors causing interference in the analysis (author's transl)].

The assay of triglycerides by an entirely enzymatic pathway was adapted for use on the GSA II Greiner. The method requires only 20 microliters of biological material. The reaction time is short: 8 min at 37 degrees C. The coefficient of variation does not exceed 6% over a period of one month. Linearity of the measurements was verified up to a level of 4 mmol/l. It was shown that the free glycerol level should be systematically measured and deducted from that of the total glycerol to assure accurate results. The stability of the plasma triglycerides was investigated as a function of the length and temperature of storage (+4 degrees C and -196 degrees C). The free glycerol increases as a function of time, whereas the level of triglycerides stays constant. Haemoglobin and various medicaments, in particular, hypolipemiants and hypoglycemiants, did not interfere.

Autoanalysis↗
PubMed1977

Inhibition by 5-(tetradecyloxy)-2-furoic acid of fatty acid and cholesterol synthesis in isolated rat hepatocytes.

Fatty acid and cholesterol synthesis in isolated rat hepatocytes were strongly inhibited by 5-(tetradecyloxy)-2-furoic acid. With either 3H2O or [2(-14)C] acetate as the labeled precursor, the concentrations of inhibitor causing 50% decrease in fatty acid and cholesterol synthesis were, respectively, less than 0.005 mM and 0.020 mM. At 0.1 mM inhibitor, citrate concentration in cells from fed rats was increased by 75%; lactate and pyruvate concentrations were decreased by 30%; ethanol oxidation was decreased by 20%; with cells from starved rats, the mitochondrial [NAD+]/[NADH] was decreased. Other parameters were unaffected. Both its potency and its specificity indicate that 5-(tetradecyloxy)-2-furoic acid will be useful in studies on the regulation of lipid biosynthesis.

Animals↗
PubMed1975

Aspartate aminotransferase and alanine aminotransferase activities in plasma: statistical distributions, individual variations, and reference values.

The determination of frequency value (percentile limits) and the classification of the different variation factors allow us to define more and more homogeneous subpopulations as we use these factors for sorting. Using as our study population those persons coming to the Centre for Preventive Medicine, we were able to: (a) Describe and measure the significance and importance of physiological variations or of variations attributed to age--the latter largely related only to excessive weight, which it seems to us is often the case. (b) Establish a classification for variation factors; the recapitulatory table should be useful to clinical chemists in helping physicians interpret a laboratory test result that falls within the zone of incertitude. (c) Suggest a preliminary group of reference values for healthy subjects, to be used in interpreting a laboratory test in this way.

Adolescent↗