Efficient method for (lyso)phospholipid class separation by high-performance liquid chromatography using an evaporative light-scattering detector.
A simple high-performance liquid chromatography method with evaporative light-scattering detection has been devised in order to separate and quantify the major phospholipid and lysophospholipid classes. HPLC analyses were performed with a diol-silica column and ternary gradient elution. Standard curves were drawn up for each of the (lyso)phospholipids involved.
Chromatography, High Pressure Liquid↗