The effect of Triton X-100, sodium deoxycholate and saponin upon the solubilization of 5'-nucleotidase, the membrane enzyme derived from pig thyroid was studied. Triton X-100 at the concentration of 0.1% did not cause enzyme solubilization, whereas at the concentration of 1.0% it caused only partial release of the former from the membranes. Saponin (1.0% concentration) brought about a marked (about threefold) increase in the enzyme activity which resulted from the exposing active loci of the enzyme, however it did not cause total solubilization of it. 1% sodium deoxycholate increased the enzyme activity by 5 times and also caused its almost total solubilization (over 90%). The results indicate the 5'-nucleotidase is strongly bound to the cell membranes and the non-ionic detergents like Triton X-100 and saponin do not fit for the solubilization of this enzyme.

5'-Nucleotidase↗

PubMedDate not supplied

[Evaluation of microsomal liver enzyme function in patients with duodenal ulcer treated with ranitidine].

Functioning of hepatocytes has been assessed with respiratory test with aminopyrine labelled with 14C radioisotope in patients with duodenal ulcer treated with ranitidine. Reversibility of changes in liver microsomal system at different intervals after the completion of therapy has also been evaluated. Altogether 30 patients with duodenal ulcer treated with ranitidine have been examined: prior to the treatment--group Ia, after one day of ranitidine administration--group Ib, within 4 weeks of therapy--group Ic, within 8 weeks of therapy--group Id, and one week after the completion of the treatment--group Ie, as well as after a 4-week follow-up period--group If. Significant decrease in the results of respiratory test has been noted in patients treated with ranitidine after both four and eight weeks. Activity of liver mono-oxidases approached baseline values after one week following the completion of therapy. The results of routine liver functioning tests in all patients of groups I and II have been within normal values. The obtained results suggest that patients treated with ranitidine should cautiously be given other medicines, especially those affecting liver functioning or metabolized in the liver.

Adult↗