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Biomedical subjects

E Postel

Publications and source records attributed to E Postel.

8 recordsLinked to original sources
PubMed1998

Results of peripheral laser photocoagulation in pars planitis.

PURPOSE: To determine the effect of peripheral retinal laser photocoagulation (PLP) on visual acuity, intraocular inflammation, and other ocular findings, including retinal neovascularization in eyes with pars planitis. METHODS: A retrospective chart review of eyes with pars planitis that had undergone PLP. RESULTS: Twenty-two eyes in 17 patients with pars planitis had undergone treatment with PLP at 2 centers. The mean age at the time of treatment was 19.3 years. Following treatment, mean follow-up was 16.3 months (range, 6 to 37 months). Mean visual acuity was 20/60 preoperatively and 20/50 postoperatively. This level of improvement was not statistically significant (P > .10), but there was a statistically significant decrease in the use of corticosteroids between the preoperative examination and the last postoperative examination (86% versus 27%, P < .05). There was also a statistically significant decrease in vitritis at the last follow-up (P = .0008) and a decrease in neovascularization of the vitreous base (P = .03) and in clinically apparent cystoid macular edema (P = .02). Epiretinal membranes were noted in 23% of eyes preoperatively and in 45% of eyes postoperatively. Only one of these epiretinal membranes was considered to be visually significant. One eye developed a tonic dilated pupil, which slowly improved. CONCLUSIONS: Although the long-term natural history of clinical findings in pars planitis is not well documented, PLP appears to decrease the need for corticosteroids while stabilizing visual acuity. It also appears to decrease vitreous inflammation. PLP has few complications and should be considered in patients with pars planitis who are unresponsive or have adverse reactions to corticosteroids.

Adolescent↗
PubMed1997

Three different genes encode NM23/nucleoside diphosphate kinases in Xenopus laevis.

Nucleoside diphosphate kinases (NDPKs) catalyse the phosphorylation of nucleoside diphosphates. In mammals, the functional enzyme is a hexamer composed of different amounts of two homologous acidic (A) and basic (B) subunits encoded by separate genes. In prokaryotes and invertebrate eukaryotes, only one cytoplasmic enzyme has been isolated. Other genes encoding chloroplastic and mitochondrial forms as well as related proteins have been cloned. Here, we show that in Xenopus laevis, as in mammals, the cytoplasmic NDPK is encoded by several homologous genes. With Xenopus laevis being a pseudotetraploid species, each monomer is encoded by two genes. The amino acid sequences are very similar, and all the differences concern amino acids located at the outer surface of the hexameric enzyme. The Xenopus genes share 82-87% identity with their human counterparts. Interestingly, in vitro, the Xenopus X1 enzyme binds to a specific nuclease hypersensitive element (NHE) of the human c-myc promoter, as does its human counterpart. X1 also binds to a single-stranded (CT)(n) dinucleotide repeat. The NHE is present in the coding strand of a pyrimidine-rich region of the 3' non-coding sequence of the Xenopus NDPK genes. We propose that NDPK is indeed able to bind to its own mRNA and prevent polyadenylation at the normal position. This could provide an autoregulatory translation mechanism. A phylogenetic tree of the vertebrate NDPK sequences supports the idea that in amphibians, as in mammals, gene duplication has resulted in functional diversification.

Amino Acid Sequence↗
PubMed1986

High-resolution 2-dimensional protein mapping of "diploid" and "aneuploid" mammary adenocarcinomas.

Two-dimensional-electrophoretic analysis has been applied to non-neoplastic mammary epithelium from eight healthy women, tumor tissue from eight "diploid" mammary adenocarcinomas, and tumor tissue from eight "aneuploid" mammary adenocarcinomas. Compared with non-neoplastic mammary epithelium, a slight numerical net non-neoplastic mammary epithelium, a slight numerical net increase of the protein spots was detected in "diploid" tumors and a marked increase in "aneuploid" tumors. Two prominent spots were present in all 16 malignant tissues examined and absent in all eight non-neoplastic tissues (silver staining method). The results suggest that a difference in the composition of cellular proteins exists both between non-neoplastic mammary cells and malignant tumor cells, and between "diploid" and "aneuploid" tumors.

Adenocarcinoma↗
PubMed1981

Biochemical analysis of normal human tissues and those of lymphoma patients by high-resolution protein mapping.

The technique of high-resolution, two-dimensional electrophoresis was applied to crude membrane protein fractions of various normal human tissue samples, and those, mainly spleens and lymph nodes, of patients with lymphomas of different types. The obtained protein maps show a high reproducibility, tissue specificity, and sensitivity to specific oncologic changes. The relevance of the latter aspect to tumor characterization is illustrated. Quantitatively, minor proteins were detected by a sensitive silver stain. The feasibility of objective, computerized quantification and evaluation of the protein maps is described.

Computers↗
PubMed1980

Periurethral bacterial flora in women. Prolonged intermittent colonization with Escherichia coli.

To determine whether the periurethral flora of women with recurrent urinary infections differ from that of women without infection, a prospective study was conducted of matched premenopausal women. Daily or alternate-day urine cultures in patients and control subjects were obtained for six months to one year, as were daily records of sexual intercourse. Periurethral colonization with the same organism preceded 29 of 31 and six of seven episodes of significant bacteriuria in patients and control subjects, respectively. Prolonged periods of intermittent colonization with the same Escherichia coli serotype were observed in both patients and control subjects. Proportion of days of colonization of E coli or other Gram-negative bacteria was not significantly different between cases and controls and was not influenced by rates of sexual intercourse. Spontaneous cure occurred despite continued sexual intercourse.

Adult↗