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Biomedical subjects

E Prahoveanu

Publications and source records attributed to E Prahoveanu.

33 records · Page 2Linked to original sources
PubMedDate not supplied

The effect of an aqueous horse-radish extract, applied as such or in association with caffeine, on experimental influenza in mice.

An aqueous horse-radish extract was repeatedly administered to mice by intranasal (i.n.) route prior to i.n. inoculation of influenza virus A/PR8/34 (H1N1). The antiviral effect of the extract--also demonstrated in vitro--was reflected by a significant decrease in the hemagglutination titers recorded in mouse lung homogenates and by a slight increase in the mean survival time of treated mice versus untreated controls. Association of horse-radish extract with caffeine led to a synergic effect only as regards the mortality rate.

Animals↗
PubMedDate not supplied

Activity of lactate dehydrogenase, acid and alkaline phosphatases in measles virus-infected monkey kidney cell cultures.

The influence of wild (Co-69) and attenuated (L-16) measles virus infection on the activity and activation energy of lactate dehydrogenase (LDH), acid and alkaline phosphatases (P-ase) was tested in R9CA monkey kidney cells at various time intervals after inoculation. With both virus strains significant changes in enzymatic activity occurred especially within the first 24 hours post inoculation. The two stains have a different influence on alkaline P-ase activity variation, which might therefore be used as a differentiation marker. Variations in LDH activation energy might be an early indicator of virus infection. LDH activity is significantly higher in cellular homogenates diluted 1:10, the dilution effect being more obvious in infected cells after a longer time interval post inoculation.

Acid Phosphatase↗
PubMedDate not supplied

Influence of 2,4,6-triamino-pyrimidyl-5-azobenzene on the activities of acid phosphatase, alkaline phosphatase and lactate dehydrogenase in cell cultures infected with herpes simplex virus type 1.

Treatment of VERO cells with 2,4,6-triamino-pyridimyl-5-azobenzene (10 gamma/ml) one hour after inoculation of herpes simplex virus type 1 limits the influence of the virus infection, consisting in the enchancement of the activities of alkaline phosphatase, acid phosphatase and LDH (especially of the latter two enzymes) and in changes in the isoenzyme patterns of acid and alkaline phosphatases and of peroxidase. In general the influence of the drug is expressed both by the fact that enzyme levels in homogenates of infected cells become closer to the values recorded in controls, and by modifications in the isoenzyme patterns altered by virus multiplication, making them more similar to those of uninfected, untreated cells.

Acid Phosphatase↗
PubMedDate not supplied

Isoenzymes of succinate dehydrogenase in measles virus-infected monkey kidney cells.

Succinate dehydrogenase (SDH) isoenzyme patterns were analyzed in R6CA monkey kidney cells inoculated with wild and attenuated measles virus. Cell homogenates were prepared either in a sucrose--Triton X-100 mixture, or in sucrose alone. In both cases the most important SDH isoenzyme pattern alterations were observed in the homogenates of cells infected with wild measles virus at 72 hours post inoculation. Similar alterations occurred in homogenates of cells infected with the attenuated strain after a delay of about 100 hours. In all the experimental variants the use of Triton X-100 allowed the visualization of a larger number of isoenzyme bands.

Animals↗
PubMedDate not supplied

Influence of sodium fluoride on isoenzyme patterns in the lung homogenates of mice with experimental influenza infection.

NaF was administered intranasally to mice- either as a single or as four daily 100-gamma doses - prior to inoculation of influenza virus A/PR8/34 (H0N1). The changes caused by drug treatment in the isoenzyme patterns of peroxidase, acid and alkaline phosphatases, succinate and lactate dehydrogenases, observed in the mouse lung homogenates, could not be correlated with the previously recorded NaF-induced reduction in hemagglutinating titers and mortality rate.

Acid Phosphatase↗
PubMedDate not supplied

The effect of an aqueous propolis extract, of rutin and of a rutin-quercetin mixture on experimental influenza virus infection in mice.

Investigations were performed on the effect of an aqueous propolis extract, of rutin and of a rutin-quercetin mixture on experimental infection with influenza virus A/PR8/34 (H0N1) in mice. Propolis extract administered intranasally 3 hours before virus inoculation led to a reduction of the HA titers recorded in the lung suspensions from infected mice, but to no reduction in mortality or increase in mean survival length. When the extract was administered 3 hours after virus inoculation, the reduction in HA titer was accompanied by a slight decrease in mortality and increase in mean survival length, Rutin and the rutin-quercetin mixture caused an increase in both HA titer and mortality.

Animals↗
PubMedDate not supplied

The effect of sodium fluoride on experimental influenza virus infection in mice.

NaF was administered intranasally to mice, either as a single 0.1-ml dose (containing 100 gamma), one hour before inoculation of influenza virus A/PR8/34 (H0N1), or as four similar daily doses, the last drug application preceding virus inoculation by 1 hour. In both cases the drug treatment led to a marked reduction in the hemagglutinating titers recorded in lung suspensions, accompanied by a lower mortality percentage and by an increase in mean survival length.

Animals↗
PubMedDate not supplied

Action of thymic polypeptides of low molecular weight on some immune reactions at tissular and humoral level in guinea-pigs and mice.

Administration of the TP2 extract containing thymic polypeptides of low molecular weight (under 10,000 daltons) was found to stimulate the delayed hypersensitivity reaction in guinea-pigs injected with BCG. It was also found to reduce the hemagglutination titre in mice injected with the APR 8 influenza virus and to raise the antiflagellar agglutinins titre in rats injected with TH Salmonella vaccine. The cellular type of immune reactions was stronger than the humoral one.

Animals↗
PubMedDate not supplied

Stimulation of anti-influenza serum antibody formation in the rat using bovine thymus polypeptide extract.

The authors show that a thymus polypeptide extract, TP1, prepared by them has the effedt of stimulating the formation of anti-influenza serum antibodies and do not show antigenic properties. The extract causes increase of serum lymphocytes. Electrophoretic analysis in polyacrylamide gel reveals a number of 11 fraction, while paper chromatography for amino acids, a number of 12 spots.

Amino Acids↗