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E Quirós

Publications and source records attributed to E Quirós.

17 recordsLinked to original sources
PubMed1995

Human immunodeficiency virus type-1 can be detected in monocytes by polymerase chain reaction.

Lymphocytes and monocytes from 25 patients infected with human immunodeficiency virus type-1 (HIV-1)--13 asymptomatic, seven with the AIDS-related complex (ARC) and five with the acquired immunodeficiency syndrome (AIDS)--were lysed and subjected to PCR with three primer pairs: SK38/SK39 (gag), SK68/SK69 (env) and SK29/SK30 (LTR). Amplified DNA was solution-hybridised with 32P-labelled probes (SK19, SK70 and SK31, respectively) and detected by PAGE-autoradiography. HIV-1 DNA was detected as follows. Asymptomatic patients: monocytes--gag 61.5%, env 100%, LTR 0%; lymphocytes--gag 100%, env 92.3%, LTR 53.84%. ARC patients: monocytes--gag 71.4%, env 57.1%, LTR 0%; lymphocytes--gag 100%, env 71.4%, LTR 71.4%. AIDS patients: monocytes--gag 80.0%, env 100%, LTR 0%; lymphocytes--gag 100%, env 60%, LTR 60%. The presence of HIV-1 DNA was confirmed in the monocyte fraction. In this cell subset, the env gene-directed primers were the most effective for amplification, whereas the LTR gene-directed primers failed to amplify HIV-1 DNA. The different pattern of amplification found in monocytes may suggest that these cells could be infected by a genetic variant of the virus.

AIDS-Related Complex↗
PubMed1995

[Activation of cell death in lymphocytes from individuals infected with HIV].

BACKGROUND: In HIV infection, T cells depletion cannot only be explained by direct viral infection. Programmed cell death (PCD), also know as apoptosis or activation-induced cell death, may be responsible for the deletion of reactive T cells that contributes to HIV induced immunodeficiency. We studied the response of lymphocytes in vitro to different polyclonal and oligoclonal (superantigen) activators, and tested whether the stimulation of T cells from individual with HIV infection lead to cell death. MATERIAL AND METHODS: Peripheral blood mononuclear cells, total T lymphocytes, TCD4+ and TCD8+ cells from 10 positive patients, 2 patients with AIDS and 10 negative individual for the virus were cultured in RPMI medium and subjected to stimulation with PHA, PWM and SEB (staphylococcus B enterotoxin). Trypan blue was used to distinguish viable from dead cells. RESULTS: TCD4+ cells from HIV-infected patients die after SEB or PWM activation (almost 40% from asymptomatic and 80% from AIDS), and do not die TCD8+ from the same individuals (around 10-15%), or T cells from seronegative controls. CONCLUSIONS: Lymphocytes from patients with HIV-infection showed as abnormal proliferative response to self-MHC class II restricted recall antigens and PWM whereas the response to PHA was conserved. Probably, apoptosis is triggered by an aberrant form of T cell activation in the mature TCD4+ cell population in HIV-infected individuals.

Acquired Immunodeficiency Syndrome↗
PubMed1994

Helicobacter pylori seroepidemiology in risk groups.

Helicobacter pylori is associated with peptic ulcer and chronic active gastritis. The response to infection can be determined by measuring serum titers of anti-H. pylori antibodies. We compared antibody titers in 612 serum samples from 570 individuals considered at risk for H. pylori infection, 170 of them are control sera from 110 adults and 60 children with no gastric alterations. The study groups were 93 institutionalized mentally handicapped children, 40 heterosexual couples, 101 HIV-sero-positive patients, 86 patients with chronic renal failure and 40 individuals (20 adults and 20 children) with symptoms associated with gastritis or gastroduodenal ulcer disease. In the adult and child control groups, 33.5% and 11.6% of the individuals had circulating anti-H. pylori antibodies. Significantly more adults (80%) and children (75%) with gastric symptoms had detectable circulating antibody titers. Elevated titers were also found in institutionalized children and in adults with renal failure.

Adolescent↗
PubMed1993

Diagnosis of HIV-1 infection by PCR with two primer pairs.

We have used two different primer pairs to assess HIV-1 infection of peripheral blood mononuclear cells (PBMC) by polymerase chain reaction (PCR). The study was carried out on 150 individuals: 50 seronegative individuals without risk behaviours for HIV-1 infection, 50 individuals with risk-behaviours but seronegative for HIV-1 and 50 patients with risk-behaviours who were HIV-1 seropositive. Discordances were found between the two primer pairs (SK38/39; SK68/69) in 3 cases. In the non-risk seronegative group, one specimen was scored positive with only one primer pair (SK38/SK39); all the samples belonging to seropositive individuals were found to be positive for HIV-1 DNA using both primer pairs; in the seronegative at risk group 2 samples were positive with only one primer pair (SK38/SK39), and 4 samples were found positive by both primer pairs (SK38/39 & SK68/69). Our study demonstrates that discrepant results take place with relatively high frequency; we propose that all specimens should be tested twice using at least two different primer pairs.

DNA Primers↗
PubMed1993

[Evaluation of DNA-HIV detection in various population groups].

BACKGROUND: To evaluate the diagnostic use of Polymerase Chain Reaction (PCR) for the detection of HIV-DNA. METHODS: We have investigated HIV-DNA in peripheral blood mononuclear cells of 150 individuals: 50 seronegative without recognized factors to acquire HIV infection, 50 seronegative that showed risk habits and 50 seropositive. For amplification we have used primers SK38/SK39 which amplify a conserved region of 114 bp from HIV's gag gen. The detection of the amplificate was performed using solution hybridization with 32P-labelled SK 19, 6% polyacrylamide gel electrophoresis and autoradiography. RESULTS: We have detected HIV-DNA at every seropositive patient (100%), 6 samples from seronegative individuals that showed risk habits and 1 sample from non-risk seronegative individuals. CONCLUSIONS: Polymerase chain reaction represents a diagnostic tool at HIV infection, specially for those patients where seroconversion can not be demonstrated by conventional methods.

DNA, Viral↗
PubMed1992

[Annual cost of microbial immunological tests for HIV-positive individuals in a hospital center].

The goal of this study is to increase the knowledge of the AIDS cost in a hospital. We have studied the HIV patients diagnosed during a year in our hospital and the charges of the serological tests performed to them. 20.8% of the tests were to study antibodies against HIV (only 20% were positive). After patient distribution between extra or intrahospital origin, we highlight that 61% of the charges are attributed to patients who do not belong to the hospital but to its catchment area. We emphasize the problem that AIDS patients are creating to hospital, being necessary that personal select only useful tests honestly.

AIDS Serodiagnosis↗
PubMed1992

[Quantification of the levels of p24 antigen and antibodies in human immunodeficiency virus infection].

BACKGROUND: Quantification and clinical evaluation of p24 antigen and anti-HIV antibody levels. METHODS: Follow up of 13 HIV infected patients (53 sera) by determination of p24 antigen, total anti-HIV antibodies, anti-p24 and anti-env antibodies by ELISA and their semiquantitation. IgG and IgM class antibody determination by immunoblot techniques. RESULTS: The highest levels of p24 have been found in WR 1 and WR 6 stages. Commonly accepted serologic pattern has not been found in 100% of our cases. Western-blot is more sensitive for the detection of anti-p24 antibodies. CONCLUSIONS: p24 antigen appearance and a decrease in anti-p24 antibody level is related to a worse clinical prognosis. p24 antigen quantification is a usefull test for monitoring AZT therapy in patients suffering from AIDS.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Immune complex p24 antigen: a new prognostic marker in human immunodeficiency virus infection.

p24 antigen (p24 Ag) is usually not found in patients in the asymptomatic phase of HIV infection, mainly because it is bound to anti-p24 antibodies (p24 Ab), with which it forms immune complexes. Nine asymptomatic patients positive for HIV-1 antibodies (stage II CDC criteria) treated with r alpha-interferon were followed (7.5 +/- 0.5 months), and a basal and final sample were tested for p24 Ag immune complexes (p24 Ag-IC), free p24 Ag, p24 Ab, CD4 count, beta 2-microglobulin, properdin B factor, and C3 and C4 complement fractions. p24 Ag-IC was detected in 55.5% of the samples, whereas free p24 Ag was detected in 5.5%. The finding of no change or an increase in p24 Ag-IC serum levels may be considered a prognostic marker. We believe that p24 Ab cannot be used as a prognostic marker unless p24 Ag-IC detection is simultaneously evaluated.

Antigen-Antibody Complex↗
PubMedDate not supplied

[Liver cirrhosis: pulmonary function].

We performed a functional respiratory examination which consisted of arterial gasometry, spirometry, diffusion capacity to CO2, alveolo-arterial gradient of O2 and pulmonary volumes to 8 patients with cirrhosis diagnosed by clinical history, laboratory exams, abdominal ultrasound and histology. Our results showed a slight obstructive pattern of peripheric airways (FMM: 88.87 +/- 8.7%) in the spirometry, no difference in arterial gases at upright and recumbent position was observed, with low values of apO2 (75.51 +/- 1.16 upright and 75.87 +/- 2.16 mmHg recumbent) without statistic significance. The gradient G(Aa) O2 increased to (30.89 +/- 1.06 mmHg). Besides there was a diffusion abnormality with a DLCO2/VA of (71.87 +/- 6.05%). Breathing 100% O2, did not change the gradient which allows us to postulate the existence of an abnormality of gaseous interchange due to shunts. We found no relationship between albumin levels and DLCO2/VO neither with pO2 in upright position; there was a relationship at recumbent position between the hepatic disorder and the arterial desaturation. We concluded that there is no significant hypoxia even with position changes, there is increase of G (Aa) O2 by shunt type disorders and that this is probably related with albumin levels.

Adult↗
PubMedDate not supplied

[Clinical and prognostic factors of mortality in upper digestive hemorrhage].

In order to investigate prognostic factors we prospectively followed 741 patients with upper gastrointestinal bleeding (UGIB). Eleven variables were identified with mortality predictive value. Hematemesis, age of 50 y/o, more than 120 heart beat/minute, 3 or more units of pack red blood cells, esophageal varices, cirrhotic liver and renal failure had independent predictive value. Our findings could help to identify high risk patients.

Age Factors↗