Biomedical subjects

E R Ashida

Publications and source records attributed to E R Ashida.

3 recordsLinked to original sources

PubMed1987

Lymphocyte major histocompatibility complex-encoded class II structures may act as sperm receptors.

Human sperm and blood cells were cocultured in vitro to determine whether specific interactions occur between gametes and blood cells. Evidence for cell type-specific sperm binding and penetration of lymphocytes is presented together with findings that suggest that either or both events involve major histocompatibility complex-encoded class II molecules on lymphocytes and a sperm ligand that is immunoreactive with antibodies to T-cell surface antigen T4. Involvement of HLA-DR is suggested by the pattern of sperm interactions with HLA-DR-positive and -negative cells and by inhibition of sperm binding to HLA-DR-positive cells by a monoclonal antibody that identifies a nonpolymorphic determinant on the HLA-DR molecule. That the complementary sperm ligand may be a T4-like structure is suggested by specific inhibition of sperm-lymphocyte binding with monoclonal antibodies OKT4 and OKT4A. The results are discussed in terms of possible roles for immunoglobulin-related structures in human fertilization and in the sexual transmission of the acquired immunodeficiency syndrome.

Acquired Immunodeficiency Syndrome↗

PubMed1986

Home intravenous immunoglobulin therapy by self-administration.

Immunoglobulin replacement therapy is required by patients with certain antibody deficiency syndromes and is finding increasing application in other immune disorders such as immune thrombocytopenia. We describe the long-term home administration of intravenous gamma-globulin by seven patients themselves using a portable infusion pump. Over a period of as long as 2 years, this has proven to be effective, safe, and cost efficient and a good alternative to hospital or physicians' office-based infusions.

Agammaglobulinemia↗

PubMed1981

Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro.

Mitogen-stimulated human T cell activation is absolutely dependent on the participation of a nonresponding accessory cell. In populations of human peripheral blood mononuclear cells, monocytes function as the requisite accessory cells. The possibility that cultured endothelial cells (EC) might also function as accessory cells was studied by examining the potential of endothelial cells to restore mitogen responsiveness to monocyte-depleted human T cells. Highly purified T cells were prepared by isolating cells rosetting with sheep erythrocytes and removing monocyte contamination by glass adherence and nylon wool column passage. When cultured at low cell density, T cells failed to respond to stimulation with various mitogenic lectins, whereas co-culture with monocytes restored responsiveness. Similarly, EC obtained from umbilical vein, pulmonary artery, and ovarian vein restored the capacity of T cells to respond to mitogens. Mitogen-stimulated T cell activation required viable endothelial cells. Moreover, effective endothelial T cell cooperation appeared to involve the establishment of cell-to-cell contact between EC and responding T cells. Accessory cell function was not a nonspecific property of all tissue culture cells as evidenced by the finding that human foreskin fibroblasts, lung fibroblasts, and HeLa cells were unable to restore responsiveness to monocyte-depleted T cells. These observations indicate that endothelial cells can support the induction of mitogen-induced T cell activation and suggest that cells lining blood vessels may play an active role in the initiation of immune responses in vivo.

Cells, Cultured↗