Biomedical subjects

E R Jenkinson

Publications and source records attributed to E R Jenkinson.

2 recordsLinked to original sources

PubMed2004

DNA replication in thermophiles.

DNA replication enzymes in the thermophilic Archaea have previously attracted attention due to their obvious use in methods such as PCR. The proofreading ability of the Pyrococcus furiosus DNA polymerase has resulted in a commercially successful product (Pfu polymerase). One of the many notable features of the Archaea is the fact that their DNA processing enzymes appear on the whole to be more like those found in eukaryotes than bacteria. These proteins also appear to be simpler versions of those found in eukaryotes. For these reasons, archaeal organisms make potentially interesting model systems to explore the molecular mechanisms of processes such as DNA replication, repair and recombination. Why archaeal DNA-manipulation systems were adopted over bacterial systems by eukaryotic cells remains a most interesting question that we suggest may be linked to thermophily.

Archaea↗

PubMed2003

Initiation of archaeal DNA replication.

The identification of DNA as the genetic material and the elucidation of its structure by Watson and Crick [Watson and Crick, (1953) Nature (London) 171, 737-738], which has its 50th anniversary this year, first suggested the simple elegance with which the problem of passing on precise genetic information from one generation to the next could be solved. Semi-conservative replication is perhaps one of the simplest biological concepts to explain and understand. However, despite an enormous amount of effort in the intervening years, details of the way in which this process is regulated and performed are still unclear in many organisms. Recent work suggests that, due to their simplicity, the Archaea may make a good model for understanding some of the aspects of eukaryotic replication that still elude us.

Archaea↗