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Biomedical subjects

E R Marchand

Publications and source records attributed to E R Marchand.

9 recordsLinked to original sources
PubMed1982

A simple teaching device for examination of the arterial and venous pulse.

A simple teaching device is described for examination of the cervical arterial and venous pulses. It is a mechanical device that simultaneously generates a visible jugular venous pulsation and a palpable carotid arterial pulsation to train medical students, physicians, and paramedical personnel. Changing of cams allows the observer to appreciate these pulsations in the normal or in a variety of abnormal states. Graphic tracings of wave forms demonstrate the accuracy of reproduction of physiologic tracings. In a test of the device, cardiologists performed significantly better than medical students in the identification of unknown pulsations. The instrument is readily accepted, simple to operate, and fully portable.

Carotid Arteries↗
PubMed1981

A new microelectrophoretic procedure for delivery of horseradish peroxidase.

A new procedure for microelectrophoretic delivery of horseradish peroxidase (HRP) is described. A dilute solution (1.5--3% w/v) of fluorescein isothiocyanate-conjugated HRP is deposited according to a standard microelectrophoretic method and the tissue reacted according to a sensitive histochemical procedure. A major advantage of this method is the reduced secondary diffusion of the enzyme present at the injection site.

Animals↗
PubMed1980

Interpeduncular nucleus afferents in the rat.

Afferents to the interpeduncular nucleus (IPN) of the rat were studied with the horseradish peroxidase (HRP) retrograde transport method. HRP was deposited microelectrophoretically in the IPN of adult rats. Major projections to the IPN originate in the medial habenular nucleus, the region surrounding the dorsal tegmental nucleus (accessory dorsal tegmental nucleus and the so-called dorsal tegmental nucleus pars lateralis), and the midbrain raphe (nucleus centralis superior and nucleus raphe dorsalis). Also, minor projections originate in the central gray and nucleus locus coeruleus. Our results indicate that the habenulointerpeduncular projection originates solely from the medial habenular nuclei and is topographically organized; medial regions of the medial habenular nuclei project to ventral portions of IPN and lateral regions project to the dorsal IPN.

Afferent Pathways↗
PubMedDate not supplied

Measurement in vivo of irreversible degradation of low density lipoprotein in the rabbit aorta. Predominance of intimal degradation.

The development of a highly sensitive method for assessing, tissue by tissue, the rates of irreversible protein degradation in vivo has allowed us to quantify low density lipoprotein degradation in the normal rabbit aorta and to localize it. The method depends upon the fact that tyramine-cellobiose, like sucrose used in previous studies, can be covalently attached to proteins, enter cells with them, and then remains trapped within the cell after the remainder of the protein molecule has been degraded. Rabbit LDL (d = 1.02 to d = 1.06 g/ml) was labeled with 125I-tyramine-cellobiose and injected into rabbits. Aortic 125I content 24 hours later served as a cumulative measure of degraded LDL (after appropriate corrections for any intact, nondegraded LDL present). Calculated aortic degradation of LDL averaged 9.4 X 10(-3) percent of the plasma pool per g aortic wet weight per day (n = 6). Intimal cells, obtained by gentle swabbing, accounted for fully 40% of total aortic degradation even though the intima represented less than 5% of the aortic mass. Autoradiography confirmed the high concentration of label in the intima. Degradation of unmodified and reductively methylated LDL were compared. The fractional rate of degradation of methylated LDL by the intima was 50% to 60% of that for native LDL, indicating that 40% to 50% of LDL degradation in the intima, predominantly endothelial cells, is mediated by LDL receptors.

Animals↗