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Biomedical subjects

E R Pille

Publications and source records attributed to E R Pille.

At least 19 recordsLinked to original sources
PubMed1989

[A new rabies vaccine in public health practice in the USSR].

A new antirabies vaccine prepared on the basis of virus grown in the ovine brain, purified from 85-90% of brain-tissue ballast substances and inactivated with beta-propilactone has been developed at the Moscow Research Institute of Viral preparations (USSR Acad. Med. Sci.). The preparation produces no neuro-allergenic effect in tests on guinea pigs. When injected to humans, the vaccine shows much lower reactogenicity than Fermi vaccine. High antigenic and immunogenic activity of the new vaccine has made it possible to work out a less intensive immunization schedule in comparison with that used for immunization with Fermi vaccine and nonconcentrated tissue-culture vaccine, viz. doses of 3 ml for 12 days or doses of 3 ml for 20 days with two booster immunizations. The preparation has been introduced into medical practice.

Adolescent↗
PubMed1988

Preparation of Hybridomas producing monoclonal antibodies against human interferon.

To prepare hybridomas secreting monoclonal antibodies (MoAb) against human alpha-interferon (alpha-IFN), BALB/c mice were immunized with IFN produced in Namalwa cells. Native alpha-IFN, as well as partially purified or on cellulose adsorbed alpha-IFN preparations were used for immunization. Seven hybridomas continuously secreting IgG against human alpha-IFN were prepared by fusion of splenocytes from immunized donors with the mouse myeloma cells. MoAb reacted in ELISA as well as in neutralization test with human lymphoblastoid, leukocytic and recombinant alpha-IFN.

Animals↗
PubMed1985

Characteristics of cellular and humoral immune responses after immunization with different rabies vaccines.

Three rabies vaccines were compared: 1. the Fermi type vaccine, a phenol-treated suspension of brain tissue from infected sheep; 2. a virus grown in sheep brain, purified from the contaminating material to 85-90% and inactivated with beta-propiolactone; 3. and the MNIIP-74 strain cultured in Japanese quail embryo cells and inactivated with beta-propiolactone. A single immunization of mice with any of the preparations resulted in about 50% inhibition of splenocyte migration after 2 days; by day 15 the inhibition was 95-98%. By day 45 the migration index returned to the initial level. Increased ability to blast transformation in splenocytes was found by day 15, reached the maximum (160 to 212% of the control level taken for 100%) by day 30, and then began to decrease. The most marked change in blast transformation was brought about by the purified cerebral vaccine, while the less marked one by the tissue culture vaccine. The titre of virus-neutralizing antibodies reached a maximum after 15-30 days, 60 days after immunization it dropped twice. The resistance of mice to intracerebral infection with the rabies virus shortly after immunization might be due to cellular protective factors, while at later intervals it correlated with the level of virus-neutralizing antibodies.

Animals↗
PubMed1985

Interferonogenicity of rabies vaccines as related to their therapeutic and prophylactic activities.

Non-interferonogenic rabies vaccine prepared from a virus grown in sheep brain and devoid of neuroallergenic factor and an interferonogenic vaccine from the same virus strain cultured in Japanese quail embryo cells have been compared. In mouse experiments both preparations appeared to have identical therapeutic and prophylactic efficacy. It seems that interferonogenicity of rabies vaccines cannot be used as a criterion of their prophylactic or therapeutic effects. The effectiveness of exogenous and endogenous interferon (IFN) in mice infected by the highly pathogenic fixed rabies virus has been experimentally demonstrated. Combined application of the vaccine and IFN or its inducer exerted the most marked therapeutic effect. Administration of IFN inducer into mouse brain was much more effective than its extraneural inoculation.

Animals↗
PubMed1983

Rabies vaccine prepared from the virus grown in Japanese quail embryo cell cultures.

Fixed rabies virus strain MNIIVP-74 was grown in Japanese quail embryo cell cultures, concentrated by ultrafiltration and inactivated with beta-propiolactone. The resulting vaccine was markedly antigenic and immunogenic for laboratory animals. Human volunteers injected with 2.0 ml vaccine on days 0, 3, 7, 14, 30 and 90 exhibited more intensive and longer antibody production than those injected daily for 14 days.

Animals↗
PubMed1981

Freeing rabies virus of the neuroallergenic factor from brain tissue.

A method of fixed rabies virus purification from infected sheep brains was proposed. It consisted of suspending the brain tissue in phosphate-buffered hypertonic (0.3 M) NaCl solution, shaking at 37 degrees C and low speed centrifugation at the same temperature. From 65 to 85% ballast proteins were removed, the neuroallergenic activity of the material was lost, but practically no losses of the virus occurred.

Allergens↗
PubMed1981

Properties of rabies vaccine freed of neuroallergenic factor from brain tissue.

Fixed rabies virus in the form of infected sheep brain suspension was freed from approx. 80% of ballast proteins, inactivated by beta-propiolactone and lyophilized. The vaccine thus obtained was devoid of neuroallergenicity when tested on guinea pigs and was highly antigenic and immunogenic. The vaccine caused no generalized reactions in volunteers; local reactions were weak and of short duration. Antibody formation was intensive in all volunteers.

Adolescent↗
PubMed1980

Investigation on the role of viral antibodies in the pathogenesis of multiple sclerosis.

An attempt was made to elucidate the role of antibodies to measles, rubella and mumps viruses in the pathogenesis of multiple sclerosis by correlating their levels in the blood of patients with clinical manifestations of the disease. Antibody titres to measles and rubella viruses were higher during exacerbations of the process and decreased during remissions. No correlation was found between the level of viral antibodies and the duration of the disease, features of its course (rapidly or slowly progressing), or the phase of the disease. The rise of viral antibody titres in multiple sclerosis appears to be a secondary process not associated with the disease aetiologically and playing no role in its pathogenesis.

Adolescent↗
PubMed1979

Properties of rabies virus (MNIIVP-74 strain) adapted to Japanese quail embryo cell culture.

The Pasteur strain of fixed rabies virus was adapted to primary cell cultures of Japanese quail embryos and designated as MNIIVP-74. In the course of adaptation the virus pathogenicity for rabbits by the intracerebral route decreased considerably and the pathogenicity for rabbits and adult white mice by extraneural routes was completely lost. After inoculation of Japanese quail embryo cell cultures, a titer of the virus in the culture fluid at 4 days was 6.25--7.0 lg LD50/ml (by the intracerebral inoculation of adult white mice). Viral antigen could be detected by immunofluorescence in the cytoplasm of approximately 60 per cent of the cells. Virus multiplication was accompanied by intensive interferon production. In cultures of BHK-21/13S cells the titer of the virus reached was 5.75 lg LD50/ml at 24 hours and about 30 per cent of the cells were affected. The MNIIVP-74 virus showed a high immunogenic activity in rabbits, guinea pigs and mice.

Animals↗
PubMed1976

The epidemiology of epidemic parotitis in the USSR.

Morbidity from epidemic parotitis in the USSR in the period from 1958 to 1972 ranged within the limits of 266.6 and 521.7 cases per 100 000 inhabitants. A 3--4 years cyclic recurrence of rises and falls in morbidity was observed. The bulk of cases of disease occur in the winter-spring period. In towns, parotitis is registered 3 times more frequently, in the mean, than in rural districts. More than 95% of patients in the USSR consisted of children under 15, while in Moscow, children aged 3--5 years were the most affected group. In Moscow in the period from February 1, 1972 to January 31, 1973, the morbidity in men was 493.2 per 100 000 and in women 339.5 per 100 000 (ratio 1:1.45).

Adolescent↗
PubMed1976

[The role of subclinical forms of epidemic parotitis in the formation of collective immunity].

A study was made of the outbreaks of epidemic parotitis in kindergartens and schools. Of 276 children aged from 2 to 13 years 58 (21%) sustained clinically manifest infection and 48 (17.4%)--a subclinical one. The clinically manifest process mostly developed in children aged from 3 to 5 years: the ratio of the clinical and subclinical forms in them constituted 1:0.5. With the progress of age there was an increase in the proportion of the subclinical forms (in 10--13-year-old children this ratio was 1:1.3). The presence of blood antihemagglutinins in the titre of up to 1:64 before the infection failed to prevent the development of the infectious process. There was no correlation between the antibody titre and the severity of the infectious process. Subclinical cases of parotitis were accompanied by less intensive antibody production than the clinically manifest ones.

Adolescent↗
PubMed1975

[Incidence of epidemic parotitis in different countries].

The authors analyzed the incidence of epidemic parotitis in various countries in 1960--1969 on the basis of the data published by the WHO. It can be supposed that there was no significant difference in the morbidity in various countries and that discrepancy in the official data of individual countries was apparently caused by the errors in recording. In the majority of the countries morbidity curve was fluctuating in character with a 4--5-year periodicity. In the countries of the northern hemisphere the greatest number of cases occurred in spring, and in the southern--in autumn. Monthly distribution of cases during the years with a high and low morbidity level displayed no significant changes.

Africa↗