Specificity of H-2-linked Ir gene control in mice: recognition of the core structure A--L in defined sequence analogues of (T,G,)-A--L.
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Alanine↗
Biomedical subjects
E Rüde
Publications and source records attributed to E Rüde.
Specificity of H-2-linked Ir gene control in mice: recognition of defined sequence analogs of (T, G)-A--L.
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Alanine↗
Production of IgG antibodies and enhanced response of nude mice to DNP-AE-dextran.
DNP-AE-dextran, prepared by the binding of DNP-epsilon-aminocaproyl residues to animoethylated dextran (predominantly alpha-1,6-linked), induced a T-independent anti-DNP antibody response in mice. However, certain differences were observed between the response to this antigen in normal andnude mice. Thus, the antibody titers of nu/nu mice from day 10 to 38 after immunization were significantly higher than those of nu/+ controls. Furthermore, DNP-AE-dextran induced a weak secondary response in nu/+ but not in nu/nu mice. For both thymusless and normal mice the production of IgG in addition to IgM antibodies to DNP-AE-dextran could be established. The former included antibodies of the IgG1 subclass which were considered to be particularly thymus dependent (1). The higher response of nu/nu mice was reflected mainly in the increased production of IgG antibodies. Under the influence of a graft-vs-host reaction, a 10-fold increase in antibody titers to DNP-AE-dextran was observed, due entirely to an enhanced IgG response.
Animals↗
Immunological responses to Salmonella R antigens. The bacterial cell and the protein edestin as carriers for R oligosaccharide determinants.
Responses in rabbits to heat-killed Salmonella minnesota R mutants (chemotypes Ra, Rc and Re) were heterogeneous with respect to the amounts and specific haemagglutinin activities (SHAA) of IgM and IgG antibodies produced to each mutant. Amounts of antibodies in IgM and IgG fractions of sera were determined by quantitative precipitation. For comparison, antibodies were also isolated using an R oligosaccharide-specific immunoadsorbent and quantitated spectrophotometrically. SHAA (haemagglutinating units/mg antibody) of IgG antibodies were similar for all three mutants. In contrast, the Ra mutant induced IgM antibodies with the highest SHAA, while the Re mutant induced IgM antibodies 10-fold lower in activity. The ratio of the amount of IgM/IgG produced was approximately 1/1 for both the Ra and the Rc mutants, while the ratio for the Re mutant was about 1/2. Salmonella R oligosaccharide-protein conjugates (chemotypes Rb2, Rc and Re) were prepared, and the responses to these antigens were compared with those to the heat-killed mutants. The conjugates were specific for the given chemotype, and they were strongly immunogenic when incorporated into Freund's complete adjuvant and administered intramuscularly. Haemagglutinin titres were relatively high, but amounts of antibodies were considerably reduced when the conjugates were administered intravenously without adjuvant. Rabbits immunized with the conjugates in the same manner as with heat-killed R mutants produced predominantly IgM responses in all three cases.
Adsorption↗