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E Ramos-Martínez

Publications and source records attributed to E Ramos-Martínez.

At least 19 recordsLinked to original sources
PubMed2006

Entamoeba histolytica: inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites.

It has been demonstrated that expression of cyclooxygenase-2 (COX-2) isoform is induced by Entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ALA) formation in hamsters. Trophozoites present in the lesion were also positive for COX-2 signal. However, no cross reactivity of the anti-COX-2 antibody with protein extract of cultivated trophozoites was found. To clarify if trophozoites are involved in PGE(2) production during ALA development, COX-2 expression was detected by in situ hybridization and RT-PCR in liver tissue from intrahepatically infected hamsters. COX-2 mRNA was in polymorphonuclear cells since 4h postinfection, and subsequently, local macrophages expressed COX-2 mRNA in a similar way. Additionally, a positive signal for COX-2 mRNA expression was detected in E. histolytica trophozoites, suggesting that, in vivo, parasite COX expression may be an important mechanism to promote inflammation.

Animals↗
PubMed2001

Liver function tests during amoebic liver abscess formation in indomethacin-treated hamsters.

Establishment of Entamoeba histolytica infection is facilitated through macrophage effector disruption by a prostaglandin E2 (PGE2)-mediated mechanism. Infection severity may be measured by weight of abscess formed. Indomethacin (Indo) treatment of infected hamsters reduced abscess weight by 30% at 7 days post-infection presumably by inhibition of PGE2. To explain reductions in abscess development by Indo treatment, we determined liver functionality in Indo-treated or untreated animals, either healthy or infected. Determinations of serum glutamic oxaloacetic (SGOT) and glutamic pyruvic (SGPT) transaminases, serum alkaline phosphatase (SAP), total serum protein (TSP), and bilirubinemia were done. SGOT, SGPT, and SAP activities showed a significant increase in their values by 600% at seven days post-infection in infected animals in both conditions; nonstatistical differences were found between animals treated or not. This increase did not correlate with the percentage of damage. Infected nontreated hamsters showed TSP levels 30% below normal group (P < 0.05). Infected Indo-treated hamsters had no significant differences compared to normal values. Infected nontreated animals showed an increase in bilirubin, particularly in indirect bilirubin, whereas infected Indo-treated hamsters showed total bilirubin values lower than normals (P < 0.05), with a decrease in direct bilirubin levels. Our results demonstrated that E. histolytica infection in hamsters produces similar abnormalities in liver function as it does in humans, and that the beneficial effect of Indo treatment on amoebic abscess development is not related with an improvement of liver functionality.

Animals↗
PubMed2001

Parathion, a cholinesterase-inhibiting plaguicide induces changes in tertiary villi of placenta of women exposed: a scanning electron microscopy study.

The objective of this work was to describe the anatomy of placentas from women who were at risk of exposure to parathion during their pregnancy, when examined with the light and scanning electron microscopes. Twenty term placentas were analyzed; 10 from women living in an agricultural area, who were at risk of exposure to parathion during their pregnancy, and 10 from women living in an urban area, not expressly exposed to pesticides. Each sample was examined with both light and scanning electron microscopes. Cholinesterase activity was significantly reduced in blood from women of the exposed group. In some placentas of women exposed to parathion, recent microinfarctions, microcalcifications and increased deposition of fibrinoid material were seen, along with a larger proportion of atypical characteristics of villi, such as bullous and balloon-like formations with nonhomogeneous surface, and other areas devoid of microvilli. These observations suggest that in chronic exposure to pesticides, the rate of atypical characteristics of placental villi increases, which could be related to changes in the fetus biology. In this study, one newborn from the exposed group showed intrauterine growth retardation and another one, some signs of hypoxia.

Adult↗
PubMed1995

[Chronic endometriosis caused by Trichinella spiralis. Report of a case].

A case of endometrial trichinosis is reported; the patient lived rear Cd. Delicias, Chih., an endemic area for trichinosis. A 38 years old obese, diabetic woman, with high blood pressure, used to eat frequently pork meat and its products; she presented with abnormal genital bleeding, abdominal pain, and pelvic congestion. Histerectomy was performed. Pathological study of the uterus showed Trichinella spiralis larvae with microcalcifications, and lymphocytic infiltration in basal endometrium. As far as we know endometrial trichinosis has not been previously described.

Adult↗
PubMedDate not supplied

Entamoeba histolytica: induction of cyclooxygenase-2 expression during amoebic liver abscess formation in hamsters (Mesocricetus auratus).

Experimental amoebic liver abscess in hamsters curses with an increase in both, systemic levels of prostaglandin E2 (PGE(2)) and local cyclooxygenase activity in liver microsomes. The cellular source of PGE(2) and the isoform of cyclooxygenase responsible are not completely evidenced. Cyclooxygenase-2 (COX-2) protein and gene expression were demonstrated on macrophages and polymorphonuclear cells as a result of Entamoeba histolytica infection in hamsters at 2, 4, and 7 days postinfection by immunohistochemistry and RT-PCR. E. histolytica trophozoites located in the lesion showed a strong positive signal for COX-2, however the enzyme was not detected in cultured trophozoites by Western blot. Our results indicate that the increment in PGE(2) is the result of COX-2 activity from cells of the reticuloendothelial system and reinforce the possibility that PGE(2) production by enzyme induction in macrophages may be a mechanism by which E. histolytica modulates the host immune response in this parasitic infection.

Animals↗
PubMedDate not supplied

[Thin-needle aspiration biopsy in thoracic lesions. Analysis of 60 cases in a General Zone Hospital].

The intention of the study was to confirm the usefulness of the transthoracic fine needle biopsy aspiration (FNBA) procedure, in a second level hospital that did not have technologically advanced equipment to locate thoracic diseases, such as computerized tomography, fluoroscopy or ultrasonography. Employing a vertical and horizontal coordinate design and posteroanterior as well as lateral radiological studies, specimens were taken from 77 patients. Of this total only 60 (77.9%) were in proper conditions to be examined; in seven (11.6%) patients a second aspiration was required. Of the total suitable specimens, malignant neoplasms were found in 46 (76.7%), and inflammatory diseases were detected in 14 (23.3%). The most frequent diagnoses were squamous cell carcinoma in 20 (33.3%), undifferentiated carcinoma in seven (11.6%), and bronchoalveolar carcinoma in another seven (11.6%). In 12 patients, it was possible to obtain tissue samples and in two patients, autopsy was performed; in all of them, the FNBA diagnoses were confirmed. Four (6.6%) patients suffered complications like pneumothorax, three were cured spontaneously, and one was treated with tube drainage. FNBA demonstrated its usefulness in a hospital with limited technological resources; 77.9% of the patients were property diagnosed with reduced cost, minimal morbidity, and without mortality.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMedDate not supplied

[Acute trichinosis. Epidemics of 166 cases in Delicias City, Chih. Diagnosis with tissue compression and staining].

The purposes of this report are: a) To show outstanding clinicopathological characteristics from the largest outbreak of trichinosis in México. b) To describe a modification to the traditional trichinoscopy through the use of compressed muscle stained with ordinary dyes an immediate observation; we have called the procedure tissular compression and dyeing technique (TCDT). The outbreak occurred in Delicias, Chihuahua, and affected 166 inhabitants who ate a not well cooked pork sausage. All patients showed malaise, 85.5 percent myalgias, and 72.7 percent facial edema; leucocytosis was seen in 75 percent of the patients, 60 percent with eosinophilia. Electromyography was performed in 21 patients in the acute phase of the illness and it was found abnormal in 85.7 percent. Only one patient died (0.6%). From 59 patients who were considered very ill, muscle biopsies were taken, and the conventional histopathological study showed larvae in 88.1 percent. TCDT demonstrated worms in 90.3 percent out of 47 biopsies; larvae were seen free and into muscle fibers with or without capsule cells. In conclusion TCDT is a simple, fast and easy reproducible procedure.

Acute Disease↗
PubMedDate not supplied

[Histologic changes in peptic disease].

The purpose of this work is to describe pathological alterations produced by the gastric juice in esophagus, stomach, duodenum, and small intestine. Histology of these segments of the gastrointestinal tract are briefly explained. Peptic esophagitis, Barrett's esophagus, chronic gastritis, duodenitis, and peptic ulcer in several sites are described.

Barrett Esophagus↗
PubMedDate not supplied

[Secondary sclerosing cholangitis. Radiologic diagnosis and anatomoclinical correlation. Report of a case].

Radiological diagnosis of secondary sclerosing cholangits was made in a patient who presented changes in morphology of the biliary tree in two consecutive post-operatory T-tube cholangiograms. The main change was a diffuse narrowness of the intrahepatic branches. Anatomical changes in post mortem examination had a good correlation with radiological images. The importance of this entity and experience from other authors in the current literature are discussed.

Bile Ducts, Intrahepatic↗