PubMed Health⌕ Search

Biomedical subjects

E Rauterberg

Publications and source records attributed to E Rauterberg.

17 recordsLinked to original sources
PubMed1991

[Hyalinosis cutis et mucosae (Urbach-Wiethe disease)--ultrastructural and immunological characteristics].

In this report on a young female patient with hyalinosis cutis et mucosae (Urbach-Wiethe disease, lipoid proteinosis), we present the clinical, immunological and ultrastructural features of this inherited disorder and discuss the differential diagnosis against other interstitial connective tissue depositions. Immunologically, the most important result was the increased amount of collagen type IV at the junction zones of epidermis, dermal vessels and appendages. This was in accordance with the ultrastructural deposition of hyalin material, mainly consisting of multiplied basal laminae at the respective junction zones. The pathogenesis (gene defect or defective gene regulation?) and therapy are discussed.

Adult↗
PubMed1984

Enzyme-linked immunosorbent assay (ELISA) with covalently bound protein on glass tubes: 1. Stable antigenicity and binding of IgG as a model antigen after repeated use.

A modification of the ELISA procedure is described. The system is based on the covalent binding of protein to glass tubes. Human IgG was used as model antigen. Optimal conditions were tested for the removal of alkaline phosphatase-labeled antibodies from their antigen. Under such optimal conditions, a regenerable system could be created which exhibited many advantages, as compared with conventional ELISAs with antigens absorbed unspecifically to plastic surfaces. The advantages are: 1. A higher density of IgG as model antigen on the solid phase, i.e., 1 molecule IgG per 94 nm2 with glass tubes as compared with 110 nm2 with polystyrene (PS) or 143 nm2 with polyvinylchloride (PVC) microtiter plates. 2. A much smaller unspecific absorption of less than 1% as compared with 39% with PS-plates or 16% with PVC-plates treated under identical conditions. 3. A higher stability of the binding of the model antigen to the solid phase, i.e., a drastically reduced protein loss of less than 6% during the first ELISA procedure (including the regeneration) and of less than 1% during the subsequent ELISA and regeneration cycles with glass tubes as compared with 46% (PS plates) or 55% (PVC plates). 4. A smaller intraday variation coefficient of the ELISAs of 4.8% with glass tubes as opposed to 9.7% or 7.5% with PS or PVC plates respectively. The system with covalently bound antigens on glass tubes could be used in at least 20 consecutive measuring cycles. In five consecutive cycles, a protein loss of less than 5% was observed and the interday variation coefficient of the ELISA reaction was smaller than 5% using the same tubes repeatedly. Our results indicate that covalent linkage of protein can improve ELISA and lead to repeatedly usable systems as long as the antigen is stable against the regeneration procedure. Such an ELISA system may be helpful with highly purified proteins.

Adsorption↗
PubMed1984

Do antibody-coated bacteria reflect local immune response in the urinary tract?

We examined whether antibody-coated bacteria (ACB) reflect local immune response or passive adsorption of immune globulins (Ig). For this purpose, bacterial subcultures from infected urines and third-party bacteria were incubated with Ig preparations. These were obtained from infected urine either by removal of Ig from ACB (dissociation in alpha-methylmannoside, 0.1 M glycine-HCl, or 3 M NaSCN) or by staphylococcal protein A affinity chromatography from the supernatant of infected urine. Coincubation of either Ig preparation with bacterial substrains of the original urine and with third-party strains (Pseudomonas aeruginosa, Proteus mirabilis, several Escherichia coli strains, enterococci, Serratia marcescens, Klebsiella pneumoniae, Staphylococcus aureus) caused coating of the bacterial surface with Ig. Coating was not measurably influenced by coincubation with various carbohydrates or by preincubation of bacteria with glucosidases. Adsorption of Ig was observed after heat denaturation and formalin treatment of bacteria and was independent of bacterial growth phase. Coating was observed both with intact IgG and with F(ab)2 fragments. We further examined the proportion of bacteria coated with IgA, IgG, and IgM in urines of 200 bacteriuric women with symptomatic urinary tract infection. The frequency histogram plot showed a continuous nonlinear unimodal distribution. Consequently, any selection of a 'normal range' is arbitrary. It is concluded that coating of the bacterial surface with Ig may not necessarily reflect local immune response. A major problem of the ACB test is definition of the normal range.

Antibody-Coated Bacteria Test, Urinary↗
PubMed1983

Does low urinary sIgA predispose to urinary tract infection?

Median urinary secretory IgA (sIgA) (ELISA technique in unprocessed urine) was 1.36 mg/liter (range, 0.29 to 2.31) in healthy female controls at various times of the menstrual cycle. It was significantly lower in women with urinary tract infection (UTI) without antibody-coated bacteria. Such decrease was found both in women with acute UTI episodes (median, 0.16; range, 0.06 to 1.71) and in asymptomatic nonbacteriuric women with a history of UTI (median, 0.52; range, 0.05 to 2.13). In the latter women, sIgA in nasal secretions tended to be low, but salivary sIgA was unchanged. Urinary sIgA was elevated significantly in individuals with nephrostomy and antibody-coated bacteria (14.4 mg/liter, range, 3.6 to 20). The study showed that locally synthesized sIgA immunoglobulins were low in the urine of individuals with recurrent UTI independent of the presence or absence of bacteriuria at the time of the study. UTI per se did not interfere with sIgA secretion as shown by high sIgA in patients with upper UTI. Low urinary sIgA may represent one factor predisposing to recurrent UTI.

Adolescent↗
PubMed1980

A new human monoclonal cold agglutinin Sa recognizing terminal N-acetylneuraminyl groups on the cell surface.

A human homogeneous IgM/K cold agglutinin (CA) Sa is described, whose corresponding antigen on erythrocytes (RBC) was abolished by neuraminidase. This indicated that the antigen was related to N-acetylneuraminic acid, similar to Pr and Gd antigens. In contrast, this antigen was only partially destroyed by proteases, whereas Pr antigens are completely destroyed and Gd antigens are not influenced by proteases. Sa antibody activity was inhibited by sialyllactose NeuAc (alpha 2 leads to 3) (alpha 2 leads to 6) Gal (beta, 1 leads to 4) Glc like anti-Gd but in contrast to anti-Pr. The corresponding antigen was associated with an RBC membrane glycoprotein fraction like Pr, Sa is one of a spectrum of human monoclonal CA against cell surface neuraminyl groups.

Aged↗