Biomedical subjects

E S Csórián

Publications and source records attributed to E S Csórián.

5 recordsLinked to original sources

PubMed1993

Comparison of two Salmonella enteritidis phage typing methods.

In the last 10 years several phage typing methods were developed for Salmonella enteritidis, leading to confusion in the predominant phage types (PT) reported from different countries. We made comparative examinations on 1487 S. enteritidis strains isolated in Hungary in 1990-1991, using two phage-sets: a modified version of the method elaborated by László et al. (here in after Hungarian method) and the system of Ward et al. (here in after Colindale method). Typability of the strains was nearly the same: 98.0% and 98.3%, the isolates belonging to 18 and 19 phage types, respectively. The Hungarian method revealed 6 (1, 1c, 1b, 1d, 7, 18), the Colindale method 5 (1, 6, 8, 21, 26) frequent phage types. In Hungary PT 1 has been predominant since 1981 and using the Colindale method 64% belonged to this type; using the modified Hungarian method this type could be divided into PT 1, PT 1c, PT 1b and PT 1d. Other frequent phage types (PT 18, PT 7) were nearly identical with Colindale types PT 26 and PT 21.

Bacteriophage Typing↗

PubMed1988

Subdivision of common Salmonella serotypes: phage typing of S. virchow, S. manhattan, S. thompson, S. oranienburg and S. bareilly.

The phage typing system elaborated for Salmonella infantis proved suitable to subclassify 633 S. virchow, 206 S. manhattan, 103 S. thompson, 30 s. oranienburg and 58 S. barielly strains. The S. virchow strains belonged to phage type 213 in 79.6%, each of six phage types occurred more frequently than 1%, and 15 phage types less frequently than 1%. The S. manhattan strains belonged to three phage types (687, 247, 547) in 91.2% and nine other types were found. The most common phage types among the S. thompson strains were 243 and 247 (52.4 and 18.4%) and eleven other types were encountered. All S. oranienburg strains derived from an outbreak and belonged uniformly to phage type 111. S. barielly strains isolated from an other outbreak fell into phage type 683.

Bacteriophage Typing↗

PubMed1988

A phage typing system for Salmonella infantis.

A phage typing method applying 9 type phages was elaborated to subdivide Salmonella infantis. Results are reported by the use of Farmer's mnemonic. Out of 4847 S. infantis strains, 4602 were of human and 245 of non-human origin. The strains were typable in 98.9%. Two phage types occurred more frequently than 20%, four phage types between 5 and 10%, seven phage types less than 5%, and twenty-eight phage types less than 1%. The strains originated from outbreaks in 28.7% and from sporadic cases in 71.3%. A total of 1320 strains examined for phage type was isolated from 4 field epidemics, 39 community outbreaks and 370 family infections. In the second version of the method two phages were substituted by two more effective ones. The phage typing method was suitable for epidemiological purposes. Inducing in vitro changes in phage types by lysogenization and plasmid acquisition, phage types 111, 113, 311, 313 and 343 changed to phage types 213, 243, 513 and 543 after lysogenization and phage types 311 and 543 to phage types 548, 565 and 885 due to plasmid acquisition.

Animals↗

PubMed1985

Epidemiological analysis of Salmonella typhi-murium infections on the basis of laboratory methods. I. Distribution of phage types and biotypes of Salmonella typhi-murium isolated in Hungary in the period 1960 to 1981.

Phage and biochemical types were determined of 34 937 Salmonella typhi-murium cultures including 31 708 human strains, 2732 animal strains and 497 strains isolated from water. Phage type 4, not typable strains (nt) and phage type 2b were predominant among the strains of human and animal origin, and nt, 4 and 2b among the strains isolated from water. The most frequent phage types and the nt strains were subdivided by biotyping and additional phages. The incidence of S. typhi-murium var. copenhagen strains was 12.4%, they belonged mainly to phage types 2b and nt. The number of S. typhi-murium isolates of human origin showed a 2-4 year periodical fluctuation between 1960 and 1981. A connection was found between the incidence of the predominant phage types (4, nt, 2b) and the periodical changes in the total number of isolates. Phage type 4, which predominated among the strains of human and animal origin till 1976, was ousted gradually by nt ones. In the period when the change in predominance was observed the number of epidemics decreased and the number of sporadic cases increased. The change in the frequency of phage types took place at the same time when the frequency of phage types changed among the strains isolated from cattle and meat-products (4----nt). The increased number of sporadic cases after 1976 refers to infections from cattle and not from poultry.

Animals↗

PubMed1985

Phage types and epidemiological significance of Salmonella enteritidis strains in Hungary between 1976 and 1983.

In Hungary, 14 819 human Salmonella enteritidis strains were isolated between 1976 and 1983. Phage type was determined of 10 132 human strains originating from 6852 foci, and of 711 strains isolated from animals and water in this period. The human strains were typable in 99.4% and they belonged into 21 phage types. Five phage types (1, 4, 7, 16 and 17) were more frequent than 1%. Phage type 7 predominated among the strains isolated between 1976 and 1980, including 65.6%-89.3% of the strains. There was a change in the prevalence of phage types from 1980-1981, as phage type 7 was ousted by phage type 1. The date of the change in the predominance of phage types coincided with the considerable increase of S. enteritidis isolates; the number of isolates was nearly fivefold in 1980 of that in 1976. Phage type 7 frequent in the first period proved to be homogeneous; the strains could not be subdivided either by the temperate phages carried by them or by other phages. The incidence of phage types 1 and 7 was nearly the same among the strains derived from animals, food, water and hygienic control examinations, and there was no temporal difference in the frequency of the two phage types as it was observed among the human strains. The human strains originated in 49.5% from outbreaks and in 50.5% from sporadic cases in the country. Of the strains examined for phage type during the eight-year period, 41.9% were isolated from 23 field epidemics, 84 community outbreaks and 757 family infections. Analysing the regional spread of S. enteritidis, the increase in the number of isolates was the highest in counties Tolna, Bács-Kiskun, Somogy and Györ-Sopron. The predominance of phage type 1 was observed in counties Békés, Borsod, Csongrád, Györ-Sopron, Hajdu-Bihar, Pest and Tolna. It was obvious in the case of county Tolna that the source of infection was contaminated egg and baby chicken. Phage type 7 predominated in counties Komárom, Vas and Veszprém. Phage type 4 circulated in counties Csongrád and Pest, phage type 17 in county Fejér and phage type 2 in county Hajdú-Bihar.

Animals↗