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Biomedical subjects

E S Hill

Publications and source records attributed to E S Hill.

6 recordsLinked to original sources
PubMed2001

Presynaptic effects of biogenic amines modulating synaptic transmission between identified sensory neurons and giant interneurons in the first instar cockroach.

Intracellular recording was used to investigate the modulatory effects of serotonin and octopamine on the identified synapses between filiform hair sensory afferents and giant interneurons in the first instar cockroach, Periplaneta americana. Serotonin at 10(-4) mol l(-1) to 10(-3) mol l(-1) reduced the amplitude of the lateral axon-to-ipsilateral giant interneuron 3 excitatory postsynaptic potentials. and octopamine at 10(-4) mol l(-1) increased their amplitude. Similar effects were seen on excitatory postsynaptic potentials in dorsal giant interneuron 6. Several lines of evidence suggest that both substances modulate the amplitude of excitatory postsynaptic potentials by acting presynaptically, rather than on the postsynaptic neuron. The fitting of simple binomial distributions to the postsynaptic potential amplitude histograms suggested that, for both serotonin and octopamine, the number of synaptic release sites was being modulated. Secondly, the amplitudes of miniature excitatory postsynaptic potentials recorded in the presence of tetrodotoxin were unaffected by either modulator. Finally, recordings from contralateral giant interneuron 3, which has two identifiable populations of synaptic inputs, showed that each modulator had a more pronounced effect on excitatory postsynaptic potentials evoked by the lateral axon than on those evoked by the medial axon. Immunocytochemistry confirmed that neuropilar processes containing serotonin are present in close proximity to these synapses.

Animals↗
PubMed1999

Dissociated adult Rana pipiens motoneuron growth cones turn up concentration gradients of denervated peripheral nerve-released factors.

During development of the central nervous system neuronal growth cones are attracted or repelled by concentration gradients of specific target-derived factors. Although evidence suggests that gradients of target-derived factors are chemotropic for adult motoneuron growth cones, a conclusive demonstration has remained elusive. Further, no preparation has been available on which to rapidly and reliably test candidate factors. The present results demonstrate that in vitro adult frog motoneuron growth cones consistently turn and grow up diffusible concentration gradients of denervated peripheral nerve-released factors over distances of almost 200 microm, and that the responsible factor/s are larger than 30 kDa. This preparation provides an excellent bioassay on which the factor/s responsible for this influence can be tested as they are isolated and characterized.

Animals↗
PubMed1998

Spatio-temporal activities in the antennal lobe analyzed by an optical recording method in the male silkworm moth Bombyx mori.

Optical recordings with a voltage-sensitive dye showed that the spatio-temporal pattern of depolarizing responses evoked by electrical stimulation of antennal nerve (AN) was non-homologously distributed in the antennal lobe (AL) of the male silkworm moth, Bombyx mori. Time courses of postsynaptic activities and GABAergic inhibitory potentials of AL neurons were individually demonstrated by pharmacological experiments, i.e. Ca2+ free and bicuculline conditions. GABAergic inhibitory potentials began with a ca. 3 ms delay from the beginning of the postsynaptic activities. Intensity of the postsynaptic activities and GABAergic inhibitory potentials were non-homologously distributed in the AL. Relatively strong postsynaptic activities and GABAergic inhibitory potentials were consistently observed in some parts of the macroglomerular complex (MGC) and/or in some ordinary glomeruli (Gs) in the medial and ventral part of the AL.

Animals↗
PubMed1998

Indirect synaptic inputs from filiform hair sensory neurons contribute to the receptive fields of giant interneurons in the first-instar cockroach.

The first-instar cockroach, Periplaneta americana, detects air movements using four filiform hair sensilla, which make synaptic connections to seven pairs of giant interneurons (GIs) in the terminal abdominal ganglion. The directional sensitivities of some of the GIs, predicted from their patterns of monosynaptic inputs, may not be the same as in the second instar or adult. Intracellular recordings were made to determine the contribution of polysynaptic inputs to the receptive fields of first-instar GIs. The ventral GI1, and the dorsal GI5, GI6, and GI7 were all found to have indirect synaptic inputs from filiform afferents. The indirect inputs were excitatory to GI1, GI5, and GI7, and inhibitory to GI6 and GI7. The indirect excitatory input to GI1 was predicted to alter qualitatively its receptive field, allowing it to respond to wind from the side of the animal, as in the adult. Inhibition was predicted to sharpen the receptive fields of GI6 and GI7. The inhibitory postsynaptic potentials reversed 6-8 mV below resting potential and were blocked by picrotoxin, indicating that they are GABAergic. Indirect excitation also altered the predicted receptive field of GI7, one of the inputs being an unusual "off-response" to movement of a filiform hair in its inhibitory direction.

Animals↗