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Biomedical subjects

E Sequeira

Publications and source records attributed to E Sequeira.

13 recordsLinked to original sources
PubMed1994

Paraspinal arteriovenous shunts in children.

Arteriovenous shunts within the spinal canal and in the paraspinal region are unusual. Spinal cord and dural arteriovenous communications have been the subject of numerous reports but paraspinal shunts causing venous congestion in the spinal canal are rarer and may present special problems in diagnosis and management. We describe three children with paraspinal arteriovenous malformations, associated with overt or potential venous congestion in the spinal canal. In each case, the lesion was successfully obliterated by endovascular therapy. Embolisation with permanent occlusive agents is an effective treatment for these rare but potentially debilitating lesions.

Arteriovenous Malformations↗
PubMed1989

Effects of fluoride on the histoarchitecture of reproductive organs of the male mouse.

The effects of sodium fluoride (NaF) ingestion in two doses (10 and 20 mg/kg body weight) for 30 days on histology and histocytometry of reproductive organs of the adult male mouse were investigated. In order to study reversibility, treatment was withdrawn for one and two months. The testes, epididymides, vas deferens, prostate, and seminal vesicle were utilized for the study by standard hematoxylin-eosin staining and an ocular eye piece and micrometer scale. NaF treatment caused severe disorganization and denudation of germinal epithelial cells of seminiferous tubules with absence of sperm in the lumina. The Leydig cell and nucleus diameters were not affected. The caput epididymis showed fewer changes than the cauda. However, epithelial cell nuclear pyknosis and absence of luminal sperm were observed. A reduction in epithelial cell height, nuclear pyknosis, denudation of cells, and absence of sperm occurred in the cauda epididymis. The vas deferens epithelium showed nuclear pyknosis, clumped stereocilia, and cell debris but no sperm in the lumen and an increase in the lamina propria. The prostate and seminal vesicles were not affected by treatment. Withdrawal of treatment caused marked recovery in the histoarchitecture of these organs. The effects of NaF treatment are therefore transient and reversible.

Animals↗
PubMedDate not supplied

A modified method for the differential staining of spermatozoa using alcoholic acidic silver nitrate.

Differential silver staining patterns have been demonstrated in mammalian spermatozoa, using an aqueous silver nitrate reagent. In the present study, mouse, rabbit, and human spermatozoa were stained using a modification of the earlier method. In the modified method, an alcoholic acidic silver nitrate stain, with subsequent differentiation in alcoholic ammonia, was used. This method enhanced the intensity of staining of the head, mid-piece, and tail. In particular, marked differentiation of the acrosomal, subacrosomal, and postacrosomal regions was obtained, which facilitated determination of acrosomal integrity. Moreover, background interference was reduced, yielding better clarity of the stained smears. The staining was carried out in the cold. This modified technique offers an advantage in the assessment of sperm morphological anomalies and membrane and acrosomal integrity, and is a simple, reliable, and useful method for the evaluation of sperm function.

Acrosome↗