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E Sikora

Publications and source records attributed to E Sikora.

65 records · Page 4Linked to original sources
PubMed1975

Some observations on hamster-derived human infection with lymphocytic choriomeningitis virus.

The sequence of events leading to the diagnosis of recent outbreaks of hamster-borne lymphocytic choriomeningitis (LCM) in New York State is briefly reviewed. Some relevant experiments on persistent LCM infection of hamsters are described, including measurements of complement levels in the affected animals. Evidence is discussed which suggests that complement has an important role in eliminating free virus in the circulation by participating in virus neutralization.

Animals↗
PubMed1975

Role of substrains in persistent lymphocytic choriomeningitis virus infection.

Two plaque-type mutants, a wild strain MB(1), and a slow-growing non-plaque-forming variant of LCM virus were compared in L cell cultures. The cultures were monitored at biweekly intervals following infection for plaque-forming virus titre and cytoplasmic and membrane specific immunofluorescence of the cells. Different patterns were obtained in each case and the relevance of these results to LCM persistence is discussed. While the LM variant continually produced virus for 35 transfers, with a cycling titre, the SC variant stopped producing detectable virus after 30 transfers. Thereafter this culture remained fluorescent and resembled the one infected with the slow-growing variant. The degree of specific immunofluorescence of both continued to cycle. The MB(1) strain stopped producing virus after the 18th transfer.

Animals↗
PubMed1975

Laboratory diagnosis of lymphocytic choriomeningitis.

Current laboratory methods for the diagnosis of lymphocytic choriomeningitis are discussed, mainly from the point of view of recent improvements. The use of sentinel guinea-pigs is suggested for the detection of lymphocytic choriomeningitis infection in animal colonies.

Animals↗
PubMed1974

Lymphocytic choriomeningitis in a hamster colony causes infection of hospital personnel.

A series of febrile illnesses, some severe, among hospital personnel was traced to an outbreak of lymphocytic choriomeningitis in a hospital research colony of Syrian hamsters. By rapid virus isolation and identification techniques, a definitive diagnosis was made 5 days after specimens were received. This outbreak emphasizes the dangers of working with experimental hamsters and tumor cell lines passaged in them.

Animals↗
PubMedDate not supplied

Polyglutamation of the antifolate anticancer drug N10-propargyl-5,8-dideazafolic acid (CB 3717) in the mouse.

The antifolate drug N10-propargyl-5,8-dideazafolic acid was shown to be metabolized to its gamma-glutamyl conjugates in mouse liver and kidneys after administration at a dose of 20 mg/kg. The tissue levels of both CB 3717 and its polyglutamates were time dependent. However, in liver the clearance of polyglutamated conjugates was 10 times slower than that of the parent compound. In liver after 48 hr up to 50% of recovered radioactivity consisted of CB 3717 polyglutamates. In addition to polyglutamate derivatives, in both liver and kidney, 72 hr after CB 3717 administration another minor CB 3717 metabolite(s), unsusceptible to conjugase treatment, was detected. The nature of this metabolite(s) is currently unknown.

Animals↗