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Biomedical subjects

E Subtil

Publications and source records attributed to E Subtil.

16 recordsLinked to original sources
PubMed1999

The case for standardization of transfusion medicine practices in French blood banks.

Despite a decrease in collection and transfusion of blood components and technical and quality assurance improvements, adverse effects of transfusion, particularly fatalities due to ABO incompatibility, remain high. In order to evaluate the immunohaematology and blood products recipient practices in French blood banks, we have mailed a questionnaire and analysed the answers. Our study shows a great variation in practices. Most blood banks (70.6%) perform a second ABO blood grouping test for patients before they consider it as valid and keep it in their laboratory's computer system. The use of faxed requisition forms for blood products (84.0%) or immunological data (89.4%) are commonly accepted by French blood banks before preparation of orders for blood components. Nevertheless, at the time of blood product delivery, 40.4% of them required the original order documents. Completion of the transfusion follow-up form for blood components is undertaken free of charge by blood banks. When immunohaematology data of a patient are in the blood banks computer system and if a hospital department wants patient's ABO rhesus (RHI) blood group card, a duplicate of this card is frequently edited. Lastly, a committee is needed to establish, as in United Kingdom, guidelines in blood products recipients practices in French blood banks. Standard operating procedures regarding organization, documentation, techniques, etc., at the blood banks and the hospital will be written and valided for each step of the process prior to blood transfusion of a patient.

ABO Blood-Group System↗
PubMed1995

New method for stem cell quantification: applications to the management of peripheral blood stem cell transplantation.

A dramatic increase in peripheral blood stem cells (PBSC) is observed after high-dose chemotherapy followed by haematopoietic growth factors. The degree of mobilisation of PBSC is quantified by the level of clonogenic cells detected by CFU assays (CFU-GM or CFU-GEMM) or CD34+ cell determination. Working under the hypothesis that, in peripheral blood, mononuclear cells in DNA synthesis (MCDS) are proliferating stem cells, we decided to detect these cells by flow cytometric measurement of their DNA content. The relations between the number of MCDS and well-known haematopoietic progenitor indicators such as CFU-GM or CD34+ cells were analysed. We studied the kinetics of recruitment of PBSC in cancer patients, treated with rmeHuG-CSF following VP-16 cytoxan chemotherapy, until the first day of leukapheresis. For the 31 patients studied the individual curves of peripheral MCDS and CFU-GM reconstitutions showed identical profiles and a good correlation was noted between the numbers of peripheral MCDS and CFU-GM (r = 0.73). In the leukapheresis product, the predictive value of MCDS was equivalent to CFU-GM for PBSC quantification (r = 0.70). In conclusion, MCDS analysis by flow cytometry provides reliable results and appears to be an alternative to CFU-GM assay or CD34+ cell determination for PBSC quantification.

Adult↗
PubMed1993

[Detection and analysis of cryoglobulins: comparative study of a population of patients and one of healthy controls].

During an 11-month period, 270 cryoglobulins were identified in 735 sera from patients versus 34 in 82 sera from apparently healthy blood donors. Western-blot analysis of the cryoglobulins was performed and cryoglobulin protein content was determined using a colorimetric assay. Comparison of findings in the two groups suggested that a substantial proportion of cryoglobulins identified in the sera of patients are quantitatively and qualitatively indistinguishable from cryoglobulins identified in healthy controls.

Bacterial Infections↗
PubMed1985

[Hematologic manifestations of cancers of the prostate].

Authors study six cases of prostatic adenocarcinoma with bone marrow metastatic involvement. Hematological manifestations can be: intravascular coagulation with anemia and myelemia. The heparin permits to control the intravascular coagulation when waiting the effect of hormonotherapy, chemotherapy or radiotherapy.

Adenocarcinoma↗
PubMed1979

Methylcellulose culture of human granulocytic progenitor cells (CFC *): results of bone marrow and blood cultures for normal subjects.

Normal values for the colony-forming ability of granulocytic progenitor cells (CFC) were established for 26 samples of normal bone marrow and 8 samples of normal blood cultured in methylcellulose. The concentration of CFC in the marrow was measured classically as the number of CFC per 10(5) nucleated marrow cells plated and, in addition, as the number of CFC per 10(5) precursors (myeloblasts, promyelocytes, and myelocytes) and per 10(5) metamyelocytes. A value approximating the total number of marrow CFC was calculated by using the number of CFC per 10(5) metamyelocytes. The normal value for circulating CFC were determined as number per ml of blood, per kg of body weight, per 10(5) circulating lymphocytes, and per 10(5) circulating granulocytes. The value of these different modes of expression are discussed. Although the values reported are not directly applicable from one laboratory to the next, they may constitute a useful reference point. Furthermore, they show that the statistical variation in the results is sufficiently small to permit the comparison of values found for pathologic samples with those determined for normal samples.

Adult↗
PubMed1978

Observation of echinocytosis in eight patients: a phase contrast and SEM study.

We report eight cases of echinocytosis discovered after screening of stained smears. The presence of echinocytes was exceptional in adults and children but was more frequent in neonates. In all cases we confirmed the presence of abnormal red cells by careful examination of the blood in wet preparations observed in phase contrast and after glutaraldehyde fixation and processing for SEM. No discernible common denominator was found to explain the presence of echinocytes in the blood of our patients. No echinocytogenic factor could be demonstrated in the fresh plasma. Echinocytes appeared quickly in patients who received exchange transfusion or transfusion of normal red cells. By contrast, in vitro incubation in the presence of substrates allowing constant ATP level never resulted in the formation of echinocytes when normal red cells were suspended in patient's plasma.

Acanthocytes↗
PubMed1978

In vitro medullary granulocytic progenitor (CFUc) cultures from 6 cases of granulocytopenias.

Medullary granulocyte progenitor (CFUc) cultures were grown in vitro from samples taken from 6 patients with toxic granulocytopenia caused by either thiamphenicol cephalothin or amidopyrine and who are now apparently cured. A decreased in the medullary concentration of CFUc has been observed and a calculated estimate shows that there was a decrease in their absolute number. A decrease in the number of CFUc per 10(5) metamyelocytes suggests a possible compensation by mitotic amplification between the stem cell and the differentiated cells. Two successive cultures have shown that the course of such medullary cultures is variable. The existence of medullary anomalies before drug toxicity as well as the practical consequences of the contrast between the apparent cure and the decrease in CFUc are discussed.

Adult↗
PubMed1976

Acute myeloblastic leukemia after immunodepressive therapy for primary nonmalignant disease.

Three patients treated with immunodepressive chemotherapy over a period of 43, 60, 38 months respectively, for primary nonmalignant disease, developed AML after cessation of chemotherapy. During the months preceding the AML outbreak, there were hematologic changes with seemed to reveal a preleukemic state. Our 3 patients, and 8 previously published cases, making a total of 11 patients, developed AML after chemical immunodepression for reasons which were neither hematologic nor neoplastic.

Arthritis, Rheumatoid↗