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Biomedical subjects

E T BOLTON

Publications and source records attributed to E T BOLTON.

At least 19 recordsLinked to original sources
PubMed1964

POLYOMA VIRUS GENETIC MATERIAL IN A VIRUS-FREE POLYOMA-INDUCED TUMOR.

The DNA-agar technique for homology studies was used to investigate of the viral genetic material. Complemetary found between the polyoma DNA and the DNA of several mammalian species. The increased complementarity of the polyoma DNA to the DNA of polyoma-induced tumor reflects an increased frequency of common poly-nucleotide sequences in the tumor DNA.

Animals↗
PubMed1963

Complementary RNA in nucleus and cytoplasm of mouse liver cells.

The rapidly labeled RNA from both the nuclei and cytoplasm of mouse liver cells can be bound specifically to mouse DNA. The bound fraction differs in base composition and metabolic stability from the bulk RNA. There is considerable cross reaction between this RNA and the DNA obtained from calf thymus.

Animals↗
PubMed1958

Mammalian viruses and Rickettsiae; their purification and recovery by cellulose anion exchange columns has significant implications.

Techniques of column chromatography with cellulose ion exchangers have been successfully applied to mammalian viruses and rickettsiae. Recovery of virus is excellent, and appreciable purification in terms of phosphorus and protein removal has been demonstrated. Elution characteristics of poliovirus (types 1, 2, and 3), and Coxsackie A9 virus are similar, whereas those of ECHO-13 and Colorado tick fever differ from them as well as from each other. Elution diagrams of preparations of ECHO-13 and polio 2 viruses grown on P(32)-labeled tissue cultures show a high degree of correlation between the distribution of titratable virus and the distribution of radioactivity. A single adsorption and elution of Q fever or epidemic typhus fever rickettsiae results in a striking degree of purification, as demonstrated by electron micrographs. The chromatographic behavior of the animal viruses and rickettsiae appears to depend more upon the chemical nature of the surfaces of these infectious agents than upon their size. The chromatographic procedure described may prove useful in the preparation of purified, P(32)-labeled, fully infectious animal viruses for further fundamental research. It may also prove useful for the removal of unwanted host materials in the preparation of vaccines.

Animals↗