Biomedical subjects

E T Lyons

Publications and source records attributed to E T Lyons.

At least 19 recordsLinked to original sources

PubMed2006

Parasite field study in central Kentucky on thoroughbred foals (born in 2004) treated with pyrantel tartrate daily and other parasiticides periodically.

Foals (79), born in 2004 on three thoroughbred horse farms (C, M, and S) in central Kentucky, were fed pyrantel tartrate daily, beginning at about 3 months of age. In addition, other parasiticides [fenbendazole (FBZ), ivermectin (IVM) alone or with praziquantel (PRAZ), oxibendazole (OBZ), pyrantel pamoate (PRT), and moxidectin (MOX)] were given periodically. All treatments were administered by farm personnel. Over a 14-month period, from May 2004 to July 2005, collections (n=989) of feces were made from the foals for determination of presence of internal parasite eggs/oocysts by qualitative and/or quantitative methods. Conclusions on drug activity are based necessarily on considering the combined effect of pyrantel tartrate and the other compounds. For small strongyles, this was related to which specific additional compound was given. Based on the percentage of foals with strongyle-egg-positive feces and/or the level of eggs per gram of feces (EPG) counts for the foals after treatment, drug activity on small strongyles was highest to lowest for MOX, IVM and IVM/PRAZ, FBZ, OBZ, PRT, and FBZ (2x for 5 days). The macrocyclic lactones (MOX and IVM) were highly superior to the other compounds. Some of the strongyle counts were high (over 2,000), especially on one farm (S), during periods when foals received only pyrantel tartrate, but a few days after administration of therapeutic dose rates of the drugs IVM or MOX, they were negative or very low. Ascarid eggs were present in feces of three foals after treatment with a combination of IVM and PRAZ. The qualitative method was more efficient than the quantitative method in detection of ascarid and strongyle eggs in the feces. Prevalence of eggs of ascarids (Parascaris equorum) was low (0, 4, and 31%), of strongyles high (80, 100, and 100%), of Strongyloides westeri very low (only one infected foal), and oocysts of Eimeria leuckarti medium to high (36, 41, and 85%) for the three farms, C, M, and S, respectively. It is uncertain whether the low ascarid prevalence was from activity of pyrantel tartrate and/or the other drugs or to a limited source of infective eggs.

Animals↗

PubMed2006

Prevalence of large endoparasites at necropsy in horses infected with Population B small strongyles in a herd established in Kentucky in 1966.

Two closed horse herds (Old Lot 4 and Field 24), infected since 1966 with Population B small strongyles resistant to thiabendazole (TBZ) and phenothiazine (PTZ), were terminated in February, March, and May, 2005. At necropsy, only the large endoparasites were identified and counted. The number of horses on pasture was 14 (239 days of age to 23 years old) for Old Lot 4 and two (3 to 20 years old) for Field 24. The time of the last antiparasitic treatment, relative to the year (2005) of necropsy, was 26 years for Old Lot 4 and 9 years for Field 24 horses. Gasterophilus intestinalis third instars (three to 113 specimens/horse) were found in all 16 horses and second instars (one to two) in two horses. Gasterophilus nasalis third instars (one to three) were recovered from five horses. Parascaris equorum infections (23 to 144) were in four horses (239 days to 4 years old). Strongylus vulgaris were present in the large intestine (one to 155) of 13 horses from 239 days to 23 years old and in the cranial mesenteric artery (two to 79) in 10 horses from 239 days to 23 years old. Strongylus edentatus were in the large intestine (two to 101) of 12 horses, ranging in age from 2.5 to 23 years old and in the ventral abdominal wall (one to 53) of six horses from 239 days to 21 years old. Specimens (seven to 872) of Anoplocephala perfoliata were in all horses. Oxyuris equi (one to 129) were recovered from seven horses (330 days to 23 years old). Thelazia lacrymalis (one to 85) infected the eyes of five horses (317 days to 11 years old).

Animals↗

PubMed2005

Field studies on endoparasites of Thoroughbred foals on seven farms in central Kentucky in 2004.

Fecal samples (n=1,584) for a parasite study were collected from Thoroughbred foals (n=349), 28 to 330 days old, on seven farms in central Kentucky during 49 monthly trips from May to October, November, or December 2004. The main purpose was to determine possible drug resistance of ascarids, also of strongyles, to ivermectin (IVM) and other commercially available compounds [fenbendazole, moxidectin (MOX), oxibendazole (OBZ), and pyrantel pamoate]. In addition, interest was on prevalence of foal parasites. Qualitative data were obtained by recording presence or absence of ascarid and strongyle eggs in feces from 7 to 25 days after treatment of some of the foals. None of the compounds completely eliminated ascarid eggs in feces of all foals posttreatment. Activity on ascarids was significantly the highest for OBZ and lowest for IVM. Activity on strongyles was significantly higher for IVM and MOX than for the other compounds. Prevalence (mean percentage of foals infected) was recorded for eggs of Parascaris equorum (39%), of strongyles (32%), and of Strongyloides westeri (2%) and oocysts of Eimeria leuckarti (28%) in feces of foals. One or more foals had infections of P. equorum on six farms (86%), strongyles on seven farms (100%), S. westeri on two farms (29%), and E. leuckarti on six farms (86%). The oldest foal infected with E. leuckarti was 301 days of age. Comparison was made on the prevalence of parasites in foals on five of the same farms in the 2004 study, which were part of a similar 14-farm project in 2003 (Lyons and Tolliver in Parasitol Res (2004) 92:400-404).

Animals↗

PubMed2005

First report and characterization of adult Uncinaria spp. in New Zealand Sea Lion (Phocarctos hookeri) pups from the Auckland Islands, New Zealand.

Two species of hookworms (Uncinaria lucasi and Uncinaria hamiltoni) have been formally described from pinnipeds, but dissimilar types are noted from these hosts. This report is the first description of hookworms (Uncinaria spp.) from the New Zealand sea lion, Phocarctos hookeri. The nematodes were collected from dead pups on Enderby Island (Auckland Islands, 50 degrees 30', 166 degrees 17') during January and February, 2004. Standard measurements of male and female hookworms were obtained, providing a general morphometric characterization of the hookworm species in P. hookeri. Considerable variations in the body length of adult hookworms were noted within the same host. The arrangement of some of the bursal rays differs from that described for U. lucasi and U. hamiltoni.

Ancylostomatoidea↗

PubMed2005

Seasonal prevalence and intensity of hookworms (Uncinaria spp.) in California sea lion (Zalophus californianus) pups born in 2002 on San Miguel Island, California.

Intestines of California sea lion (Zalophus californianus) pups (n= 204), born in 2002 on San Miguel Island, California, were examined for hookworms (Uncinaria spp.) as part of a seasonal mortality study from June through December 2002 and January 2003. The investigation was planned to coincide with most of the previously established hookworm infection period of the pups. Prevalence of hookworms in dead pups was 100% for each month of the study. The geometric mean intensity of infections per month was: 94.03 (n=30) for June, 629.09 (n=50) for July, 319.90 (n=31) for August, 159.90 (n=30) for October, 109.03 (n=30) for November, 37.84 (n=24) for December 2002 and 11.05 (n=9) for January 2003. In addition to the temporal pattern, the infection intensity was higher for pups in good condition and for male pups. An inter-year comparison of hookworm counts from dead pups collected in July of 1996, 2000, and 2002 also demonstrated higher intensity in pups in better condition but sex-differences in intensity were inconsistent across years. The inter-year comparison also demonstrated higher intensities in dead pups collected from portions of the rookery with sandy substrate versus rocky substrate. No annual differences in intensity were found after adjusting for substrate and condition.

Ancylostomatoidea↗

PubMed2005

Effect of moxidectin selection on the genetic variation within Cylicocyclus nassatus based on amplified fragment length polymorphism (AFLP).

Cyathostomins are among the most important intestinal nematodes of horses, yet, the literature on the molecular genetics of these worms is scarce. In this study, the technique of amplified fragment length polymorphism (AFLP) was applied to study the genetic diversity as well as to determine the effect of moxidectin selection on the population genetic diversity for Cylicocyclus nassatus, one of the most common cyathostomin species. Genomic DNAs from 30 individual male worms were used from each of two populations: an avermectin-milbemycin (AM)-naive population (Population-S) and a population derived from Population-S following 21 treatments with moxidectin (Population-Mox). Three selective primer pairs were used for each worm, yielding a total of 229 AFLP markers. Calculation of average pair wise Jaccard indices revealed a high degree of genetic variation within both populations using all three primer combinations. In addition, selection by moxidectin during a 3-year period caused a significant decrease in the level of genetic diversity as evidenced by analysis of AFLP markers for two primer combinations but not for the third. A dendrogram of relationships among individuals based on AFLP markers did not show a clear classification of individuals in separate groups. It was concluded that a high degree of genetic intrapopulation variation exists in C. nassatus and that moxidectin selection has a significant effect on the genetic composition of C. nassatus.

Animals↗

PubMed2005

Photomicrographic images of some features of Uncinaria spp (Nematoda: Ancylostomatidae) from otariid pinnipeds.

Photomicrographs of several morphologic features of hookworms (Uncinaria spp) from northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups are presented. The main purpose is to show and describe some physical characteristics of hookworms from the two hosts; it is not to decide from these attributes whether the Uncinaria spp are the same species. The number of species of Uncinaria in pinnipeds is uncertain and specimens need to be examined from the various infected seals and sea lions before the taxonomy of these parasites can be clarified. Information in the present paper should aid in this determination.

Ancylostomatoidea↗

PubMed2005

Historic importance of some aspects of research by O. Wilford Olsen on hookworms (Uncinaria lucasi) in northern fur seals (Callorhinus ursinus) and Steller sea lions (Eumatopias jubatus) in 1951 on St. Paul Island, Alaska.

The purpose of this paper is to disseminate, more widely, certain historically important aspects of research by O. Wilford Olsen on hookworms (Uncinaria lucasi) in northern fur seals (Callorhinus ursinus) and Steller sea lions (Eumatopias jubatus) in 1951 on St. Paul Island, Alaska. Highly meaningful facets of the research were recorded only in a report with limited availability by Olsen in 1952. Portions of his research presented here include measurements of hookworm free-living infective third-stage larvae (L3) and adults, photographs of bursae, and the conclusion that the species of hookworms (U. lucasi) is probably the same in both pinniped hosts. This information is especially important because very little has been published on taxonomy of hookworms in E. jubatus.

Alaska↗

PubMed2004

Prevalence of parasite eggs (Strongyloides westeri, Parascaris equorum, and strongyles) and oocysts (Emeria leuckarti) in the feces of Thoroughbred foals on 14 farms in central Kentucky in 2003.

Prevalence of internal parasites was determined by fecal examination for eggs and oocysts in Thoroughbred foals in central Kentucky in 2003. Fecal samples were examined from 733 foals on 14 farms. This included 70 trips to the farms and a total of 2,346 fecal samplings. Monthly collection of fecal samples was begun for four farms in February, six in March, three in April, and one farm in May. Termination of the study for all farms was the end of July. A criterion was that the foals be at least 10 days old for initial samplings. If available, the same foals were sampled each time, in addition to foals born in the interim between farm visits. Ages of the foals for the complete study varied from 10 to 223 days. Prevalence (mean %) was determined for eggs of Strongyloides westeri (1.5%), Parascaris equorum (22.4%), and strongyles (27.6%) and for oocysts of Eimeria leuckarti (41.6%) in feces of foals. Foals had infections of S. westeri on six farms (42.9%), of P. equorum on 12 farms (86%), and of strongyles and E. leuckarti on all 14 farms (100%).

Animals↗

PubMed2004

Penetration of the small intestine of a California sea lion (Zalophus californianus) pup by adult hookworms (Uncinaria spp).

During a study on the mortality of California sea lion (Zalophus californianus) pups born on San Miguel Island, California in 2002, two adult female hookworms (Uncinaria spp) were found penetrating the serosal surface of the intestinal wall and protruding into the peritoneal cavity of one pup. Documentation and a description of this unexpected finding and associated lesions are presented here. Also, adult hookworms were found in the peritoneal fluid of two other dead Z. californianus pups.

Ancylostomatoidea↗

PubMed2003

Field test data on small strongyles in evaluation of activity of fenbendazole given once a day for 5 consecutive days to thoroughbred yearlings on two farms in Kentucky in 2002 and 2003.

Fenbendazole (FBZ) suspension was administered intraorally at the dose rate of 7.0-10.3 mg/kg once a day for 5 consecutive days to 58 thoroughbred yearlings on two farms in central Kentucky in April, 2002. The average dose rates of drug given to groups of colts and fillies on each farm were 7.8-8.5 mg/kg. Only 3 of the yearlings had negative counts of strongyle eggs per gram of feces (EPGs) after treatment which was at 8.4, 8.7, or 9.4 mg/kg; the pretreatment EPG counts were low (10-30). Reduction of EPG values at the highest dose rates was 0% (at 9.5 mg/kg) and 78% (at 10.3 mg/kg). This study was repeated in April, 2003 in 38 thoroughbred yearlings on one of the two same farms used in the 2002 research, but all horses were treated at the same dose rate (10 mg/kg) of FBZ paste once daily for 5 consecutive days. Only 1 of these yearlings had a negative EPG count after treatment, but this value was also negative before treatment. Reductions of EPG counts after treatment ranged from 0% to 85% (mean =22%) for the colts and from 0 to 63% (mean =14%) for the fillies. Examination of cultures of fecal samples from these yearlings revealed that only small strongyle larvae were present. There was obvious FBZ-resistance of the small strongyles in yearlings on both farms at the dose rates used.

Animals↗

PubMed2003

Ascaridoid nematodes and associated lesions in stomachs of subadult male northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska: (1987-1999).

Stomachs from subadult male northern fur seals (SAMs) (Callorhinus ursinus) (n = 5,950) from St. Paul Island, Alaska, were examined from 1987 to 1999 for lesions and parasites. On gross examination of these stomachs, parasite nodules were evident in 92% of the stomachs and active and healing ulcers were found in 14% and 10%, respectively. Prevalence of nematodes from 1987 to 1997 was 88% for 5,700 SAMs for which numbers of parasites were estimated but not identified to the genus level. All nematodes recovered from 250 SAMs examined in 1998 (n = 124; 99% infected) and in 1999 (n = 126; 91% infected) were identified and counted. Prevalences in 1998 and 1999 were 5% and 0% for Anisakis spp., 52% and 18% for Contracaecum spp., and 96% and 89% for Pseudoterranova spp., respectively.

Alaska↗

PubMed2002

Observations in 2001 on hookworms ( Uncinaria spp.) in otariid pinnipeds.

Uncinaria spp. were recovered from the milk of California sea lions ( Zalophus californianus) collected from the: (1) teats of a cow just after parturition (one parasitic third-stage larva, L(3)), (2) stomach of her nursing pup (two L(3)), and (3) stomach of a dead pup about 2 days old (one L(3), one headless, probably L(3), and four L(4)) on San Miguel Island, California in May 2001. This, in addition to earlier research, indicates transmammary transmission of hookworms in this host. Uncinaria spp. were found in dead northern fur seals ( Callorhinus ursinus) in the: (1) intestines of 2 of 75 pups (either one or two adult specimens in each infected pup) and (2) ventral abdominal blubber of 3 of 78 subadult males (one to seven L(3) in each infected seal) on St. Paul Island (SPI), Alaska in July and August 2001. These findings verify the low current prevalence of Uncinariaspp. in fur seals on SPI. Rectal fecal samples taken from 50 live Steller sea lion ( Eumetopias jubatus) pups, about 1 month old, on Rogue Reef in Curry County, Oregon in July 2001, were all negative for the eggs of Uncinaria spp. The apparent zero infection rate in these pups is possibly because the rocky terrain of this rookery is not suitable for hookworm transmission.

Ancylostomatoidea↗

PubMed2002

World association for the advancement of veterinary parasitology (WAAVP): second edition of guidelines for evaluating the efficacy of equine anthelmintics.

These guidelines have been designed to assist in the planning, operation and interpretation of studies which would serve to assess the efficacy of drugs against internal parasites of horses. Although the term anthelmintic is used in the title and text, these guidelines include studies on drug efficacy against larvae of horse bot flies, Gasterophilus spp., which are non-helminth parasites commonly occurring in the stomach of horses. The advantages, disadvantages and application of critical and controlled tests are presented. Information is also provided on selection of animals, housing, feed, dose titration, confirmatory and clinical trials, record keeping and necropsy procedures. These guidelines should assist both investigators and registration authorities in the evaluation of compounds using comparable and standard procedures with the minimum number of animals.

Animals↗

PubMed2001

Current prevalence of adult Uncinaria spp. in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California, with notes on the biology of these hookworms.

A prevalence survey for hookworms (Uncinaria spp.) was done in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, CA, in 2000. Intestines of dead pups were examined for adult hookworms in July. These parasites were found in 95% of 20 fur seal pups and 100% of 31 sea lion pups. The number of hookworms varied from 4 to 2142 (mean = 760) in fur seal pups and from 20 to 2634 (mean = 612) in sea lion pups. A direct relationship was evident between body condition and number of hookworms in the pups; that is, pups in poor condition had fewer hookworms than those in good condition. There was a decline in the number of hookworms in sea lion pups in 2000 compared to collections in 1996. Eggs of Uncinaria spp. were found in rectal feces (collected in late September and early October) of none of 35 (0%) live fur seal pups and 41 of 48 (85%) live sea lion pups. Packed cell volume values, determined for most of the same live pups, were essentially normal for C. ursinus but were much lower than normal for most Z. californianus. Hookworm larvae were not found in blubber of fur seal and sea lion pups or in rookery sand in July. Rookery sand, positive for live hookworm larvae when put in a refrigerator, was negative at removal 2.5 years later. The average number of eggs in utero of female hookworms was 285 for three specimens from a fur seal pup and 281 from three specimens from a sea lion pup. One hookworm larva was recovered from milk stripped from the teats of a stranded Z. californianus female at The Marine Mammal Center, Sausalito, CA.

Ancylostomatoidea↗

PubMed2001

Transmission of endoparasites in horse foals born on the same pasture on a farm in central Kentucky (1996-1999).

Research carried out during the last 4 years (1996-1999) of an 11-year study of the prevalence of internal parasites naturally transmitted to horse foals born on the same pasture on a farm in central Kentucky is presented here. Horses in this herd were not treated with any antiparasitic compound for over 20 years except for a replacement stallion in 1994. A total of 22 species, including 12 species of small strongyles, were recovered in the 4-year period. Transmission patterns of all species (n=35) of endoparasites recovered are compared for the 11-year study. Some of the changes were an increase in number of Thelazia lacrymalis and Anoplocephala perfoliata and a decrease in Gasterophilus intestinalis, Parascaris equorum, and Strongylus vulgaris. Clinical problems associated with parasitism were not observed in any of the 92 foals in the long-term investigation.

Animals↗

PubMed2001

Continuance of studies on Population S benzimidazole-resistant small strongyles in a Shetland pony herd in Kentucky: effect of pyrantel pamoate (1992-1999).

Research on benzimidazole-resistant Population S small strongyles began in a Shetland pony herd in 1974 at the University of Kentucky and has continued for over 25 years. The present update, for the period 1992-1999, evaluated activity of pyrantel pamoate (PRT) in field tests in the pony herd. Additional critical tests with PRT and oxibendazole (OBZ) were done in foals born in the herd. Activity of PRT was initially excellent in field tests, based on epg/lpg count data, but declined rapidly during the second full year of pyrantel treatments. Critical test data for small strongyles indicated efficacies of PRT were about 60% at the beginning of the present observations and this intermediate level of removal continued throughout the seven-year period except for 1994 (75%). Unusual was the finding that field test epg/lpg data on small strongyles indicated much lower activity of PRT than found in worm count data in critical tests. The previously reported ineffective activity of OBZ on this population of small strongyles continued. Data are presented on prevalence and drug activity on several species of internal parasites besides small strongyles.

Animals↗

PubMed2001

Strongyloidiasis in a Cope's grey tree frog (Hyla chrysoscelis).

A male Cope's grey tree frog (Hyla chrysoscelis) died spontaneously with ventral subcutaneous edema and was necropsied. Thickening of the intestinal mucosa was observed histopathologically, with villous atrophy and intraepithelial nematodes present. Adult female Strongyloides sp. nematodes were isolated from the fixed intestinal tract, the first time this nematode genus has been recovered from this frog genus. Intestinal strongyloidiasis should be considered as a cause for protein-losing enteropathy and death in frogs.

Animals↗