PubMed Health⌕ Search

Biomedical subjects

E Tapp

Publications and source records attributed to E Tapp.

5 recordsLinked to original sources
PubMed1977

HBsAg and HBcAg in the livers of asymptomatic hepatitis B antigen carriers.

Asymptomatic blood donors with persistent HBs antigenaemia have a variety of histological lesions in the liver, and serial biopsies indicate that, in some, these lesions may progress. The immunoperoxidase technique was found to be a sensitive method for the histological demonstration of HBsAg and HBcAg. Livers showing minor histological changes contained more HBsAg than those with active lesions and there appears to be an inverse relationship between the amount of HBsAg in the liver and the severity of the histological damage. In the carriers who had more than one biopsy, the presence of stainable HBsAg, irrespective of the initial histological diagnosis, was associated with a greater likelihood of progression of the histological lesion. HBcAg was found only in the hepatocyte nuclei of carriers with chronic aggressive and chronic persistent hepatitis.

Carrier State↗
PubMed1975

Orcein staining of hepatitis B antigen in paraffin sections of liver biopsies.

Liver biopsies from 97 hepatitis B antigen (HBsAG)-positive patients were stained by a modified orcein method described by Shikata et al (1974) in order to detect the antigen in liver tissue. The results were consitently negative in acute hepatitis, but positive in nearly two-thirds of biopsies from 53 patients with chronic liver disease. The distribution of positive staining was frequently irregular so that there is a problem of sampling error in needle biopsies. The deposits were seen in the cytoplasm of liver cells and occasionally in Kupffer cells, but never in nuclei. There was an inverse relationship between staining and parenchymal necrosis. Biopsies from asymptomatic HB(s)Ag carriers were often strongly positive, as were "ground-glass" hepatocytes in carriers and patients with chronic liver disease. The mechanism of staining is unclear but may be related to the presence of disulphide bonds in (HBsAG. The technique is simple and of use both in fresh and stored material.

Acute Disease↗