PubMed Health⌕ Search

Biomedical subjects

E Thüroff

Publications and source records attributed to E Thüroff.

10 recordsLinked to original sources
PubMed1997

Molecular Probe Data Base (MPDB).

Molecular Probe Data Base contains detailed information on synthetic oligonucleotides with a sequence of up to 100 nucleotides. This database prevalently contains information related to human oligonucleotides used in diagnostics. Molecular Probe Data Base has been made available on-line through the Internet by means of Network Information Retrieval (NIR) tools since 1993. Two years ago, a collaboration with EMBL Data Library was also set up, so that the Molecular Probe Data Base has been integrated with other molecular biology data banks in the sphere of the SRS WWW network browser. In this paper, the most recent enhancements and the current status of the Molecular Probe Data Base are briefly presented.

Base Sequence↗
PubMed1996

Molecular probe data base (MPDB).

The molecular probe data base (MPDB) contains detailed information on synthetic oligonucleotides, including their identification, target genes, applications and bibliographic references. It is available on-line through Internet and can be searched by using Network Information Retrieval tools. In this article the most recent enhancements of MPDB, both in terms of data contents and new ways of access, are described. These include a recently established collaboration with EMBL Data Library, in the sphere of SRSWWW network browser, in view of a better integration of MPDB with other molecular biology databases.

Computer Communication Networks↗
PubMed1994

Molecular Probe Data Base (MPDB).

This paper provides an update on the contents and structure, forms and mode of data distribution of the Molecular Probe Data Base (MPDB), a database that collects and provides on-line information on the sequence, target gene, applications and bibliographic references of synthetic oligonucleotides. The recent data extension and the new means of accessing the database are discussed.

Base Sequence↗
PubMed1992

Drosophila salivary glands exhibit a regional reprogramming of gene expression during the third larval instar.

In D. virilis salivary glands transcripts of two early gland protein genes, Egp-1 and Egp-2, which encode putative secretory proteins, accumulate in all cells from the first to mid third larval instar. Subsequently the transcripts disappear from the cytoplasm of the corpus cells, but not from their nuclei, where they accumulate at the chromosomal site of their synthesis. In the collum cells, however, Egp-transcripts continue to be detectable in the cytoplasm until the end of larval life. In the salivary glands of transgenic D. melanogaster the presence of a Egp-1/lacZ fusion protein shows the same regional shift as the cytoplasmic Egp-transcripts in D. virilis. We predict that the expression of Egp-genes is related to an early secretory function of the larval salivary glands which is executed by all cells during earlier larval stages but becomes restricted exclusively to the collum cells during the third larval instar.

Amino Acid Sequence↗
PubMed1990

Genes from two intermoult puffs in Drosophila virilis polytene chromosomes are differentially transcribed during larval development.

Genes from two Drosophila virilis intermoult puffs were isolated by microcloning. From puff 16A on the X-chromosome a 2.9 kb DNA fragment was obtained, which hybridizes with three transcripts. Two of them represent the mRNAs for larval glue proteins. They are found in different abundancies in third larval instar salivary glands, but also in minor amounts in midgut and in fat body. In puff 55E on chromosome III two genes were identified. They are transcribed exclusively in salivary glands during all three larval instars. Therefore, their products must be related to another gland-specific function, which is sustained throughout larval life.

Animals↗
PubMed1988

A non-conserved sequence in the 5'region of the CYH2 intron from Saccharomyces cerevisiae controls splicing efficiency of the pre-mRNA.

The CYH2 gene from Saccharomyces cerevisiae containing one 510 bp intron is spliced inefficiently. We have shown previously that a non-conserved sequence within the intron is responsible for this low splicing efficiency. Using synthetic oligonucleotides comprising the identified region we show in this report that a very short region contains the specificity to act negatively on the splicing efficiency of the CYH2 gene. Furthermore, this sequence influences the splicing efficiency only when it is placed close to the 5' splice site of the gene. Investigations with chimeric CYH2/beta-actin genes show that this sequence acts independent from its natural surroundings. We propose that this sequence might interact with splicing factor(s).

Base Sequence↗
PubMed1986

Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae.

To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing whereas intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.

Base Sequence↗
PubMed1986

Molecular cloning of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe.

Using the structural gene for the ribosomal protein L3 from Saccharomyces cerevisiae as a probe, we isolated a homologous fragment from genomic DNA of Schizosaccharomyces pombe. Analysis of the plasmid carrying this fragment by hybridization selection and 2D-electrophoresis revealed a 31 kDa ribosomal protein. Transformation of the vector pDB248x containing this fragment into Schizosaccharomyces pombe leads to an increased level of mRNA suggesting that we have cloned the entire and actively transcribed gene.

Cloning, Molecular↗
PubMed1984

Inhibition of ribonuclease activity during RNA synthesis in isolated yeast nuclei by cadmium.

We have developed an efficient transcription system in isolated yeast nuclei. If MnCl2 is substituted by CdCl2, degradation of newly synthesized RNA is markedly reduced. This effect is due to the inhibition of nuclear ribonuclease activity, since microsomal ribonuclease activity is less affected by the cation. The extent to which the addition of CdCl2 to the in vitro transcription assay inhibits ribonuclease activity is demonstrated by the measurements of the size of newly synthesized RNA. Efficient RNA synthesis in this system is not affected up to a concentration of 0.1 M CdCl2.

Cadmium↗