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Biomedical subjects

E Tolvanen

Publications and source records attributed to E Tolvanen.

At least 19 recordsLinked to original sources
PubMed1996

Epidemiology and traumatology of injuries in elite soccer: a prospective study in Finland.

A prospective study of male soccer injuries among 12 teams playing at the highest competition level was carried out in Finland in 1993. Overall, two out of three players were injured during the whole season. The injury incidence per 1000 playing hours among injured players and all players during games was higher than during practice, 14.2 vs. 11.3 and 2.3 vs. 1.8, respectively. The lower extremity was involved in 76% of the injuries. Thigh injuries were most frequent (22%), whereas overuse injuries were scarce (6%). Eighteen per cent of the injured players needed surgery and in most cases (58%) the reason for surgery was a knee injury. Sixteen per cent of all injured players were absent from soccer for more than 1 month after the injury. The mean absence time was 17 days for all and 84 days for operatively treated players.

Adolescent↗
PubMed1991

Zuclopenthixol, a combined dopamine D1/D2 antagonist, versus haloperidol, a dopamine D2 antagonist, in tardive dyskinesia.

Animal data suggest that a D1 antagonistic component in neuroleptic drugs counteracts development of dopamine supersensitivity and of tolerance to cataleptic effect. This has led to the hypothesis that neuroleptics with D1 antagonistic activity should cause a better suppression of tardive dyskinesia (TD) and less rebound aggravation after withdrawal than pure D2 antagonists. In this study the effect of zuclopenthixol (mixed D1/D2 antagonist) and haloperidol (D2 antagonist) was evaluated in chronic psychotic patients with TD. Fifteen patients completed a randomized crossover study with blind evaluation of TD and parkinsonism. The test medications, haloperidol and zuclopenthixol, caused a significant suppression of TD and a significant increase of parkinsonism. No significant differences between haloperidol and zuclopenthixol were observed. No TD aggravation was seen. The lack of differences between the mixed D1/D2 antagonist and a D2 antagonist suggest that tolerance and DA supersensitivity play no or a minor role for development of TD.

Adult↗
PubMed1991

Silver impregnation and immunohistochemical study of nerves in lumbar facet joint plical tissue.

Impingement of plical synovial tissue in a facet joint could cause pain. Plical tissue was removed during surgery for recurrent disc herniation or spinal stenosis. The presence of nerves was studied with silver impregnation, immunofluorescence, and avidin-biotin-peroxidase complex (ABC) immunostaining. Heterologous antisera to protein gene product (PGP) 9.5, substance P, calcitonin gene-related peptide (CGRP), and galanin were used to stain nerves. After silver impregnation, nerve-like structures were observed perivascularly. Such nerves located close to blood vessels were also immunoreactive for PGP 9.5, a more general cytoplasmic neural marker, whereas only few perivascular small varicosities were seen with antisera to substance P and galanin and none with antiserum to CGRP. In addition, PGP-9.5-, substance-P-, and galanin-immunoreactive nerves were occasionally seen very near to fat globules. Very few peptide-immunoreactive nerve varicosities were seen with immunofluorescence, and none of the PGP-9.5-immunoreactive nerves that were observed with ABC immunostaining were immunoreactive for neuropeptides as well. One mechanism for pain production could be mechanical compression of fatty tissue, but it is considered more likely that nerves in this particular tissue are mainly involved in local vasoregulation and that they are not sensory nociceptive nerves.

Adult↗
PubMed1990

Immunohistochemical demonstration of subclasses of inflammatory cells and active, collagen-producing fibroblasts in the synovial plicae of lumbar facet joints.

Plical tissue removed perioperatively from lumbar facet joints was studied immunohistochemically with the avidin-biotin-peroxidase complex (ABC) method for a putative presence of various subclasses of inflammatory cells and active collagen-producing fibroblasts. These methods have previously been used successfully for demonstrating specific inflammatory cells in rheumatoid arthritic synovium and cervical ligament and for studying regeneration of connective tissue, respectively. An overt inflammatory cellular response could not be demonstrated, as B lymphocytes were totally absent and no lymphocyte activation markers, either interleukin-2 receptor or immune-response-associated Ia antigens were expressed. Most of the immunoreactive cells observed were CD11b monocytes, and even these were only scattered in the connective tissue. Occasional activated collagen-producing fibroblasts were seen, suggesting in these few cases a possible regenerative response of the collagenous tissue, maybe induced by an impingement phenomenon, as such cells are not normally seen in resting connective tissue.

Collagen↗
PubMed1990

Nerves in inflammatory synovium: immunohistochemical observations on the adjuvant arthritis rat model.

Previous evidence has been presented that neurogenic input may influence adjuvant induced arthritis (AA) in rats. We now present evidence of alterations in synovial nerves in AA. Nerves were studied in well perfused and fixed rats, using immunohistochemistry with the sensitive avidin-biotin peroxidase complex (ABC) method and heterologous antisera to cytoskeletal protein gene product 9.5 (PGP) and the neuropeptides substance P and calcitonin gene related peptide (CGRP). The innervation of synovium was compared in normal rats and rats with AA. Observations concordant with what has been reported for neuropeptide nerves in the synovium of patients with rheumatoid arthritis (RA) are presented. It has been suggested that neural peptide substances are reduced in nerves of synovium from patients with RA. In the AA rat a specific reduction of lining zone and sublining nerves in the synovium was noted. The AA rat model is very suitable for studying the involvement of synovial nerves in arthritis, permitting optimal preservation of immunoreactive neural epitopes.

Animals↗
PubMed1990

Primary inflammatory reaction in synovial fluid and tissue in rabbit immobilization osteoarthritis.

The kinetics and composition of the primary cellular inflammatory process were studied in the synovial fluid (SF) and synovial tissue (ST) compartments of a rabbit knee immobilization osteoarthritis model. Immobilization induced rapid migration of neutrophils (59% +/- 26% of all cells) into SF in three days, which was accompanied by nonspecific esterase-positive monocytes (71% +/- 8% of all mononuclear cells). This finding suggests that non-specific inflammation mediated by phagocytic leukocytes predominates the cellular response in the SF compartment. In contrast, morphometric analysis of ST proper showed an inflammatory mononuclear cell response, the intensity of which diminished over time during the study period from Day 3 (416 +/- 59 cells per 0.049 mm2 ST tissue) through Day 10 (305 +/- 32 cells) to Day 35 (174 +/- 36 cells). A dotlike T-pattern alpha-naphthyl acetate esterase (ANAE) was found in the T-cell-dependent areas of secondary lymphatic tissue in the spleen, enabling immunocytologic ANAE marker studies. The ST response in situ was predominated by tissue macrophage, though infiltrates rich in T lymphocytes were present in the immediate sublining stroma. There was a significant correlation between the intensity of the SF cell response (total recovery) and the percentage of neutrophils, but there was no correlation between the intensity of the ST response and the proportion of T lymphocytes. These T-cell accumulations together with the local proliferation of fibroblastlike lining cells and stromal fibroblasts suggest that the primary inflammatory cell response is not caused by either wear and tear or mechanically by cartilage fragments.

Animals↗
PubMed1987

Lymphocyte subpopulations, activation phenotypes, and spontaneous proliferation in tuberculous pleural effusions.

The state of lymphocyte activation in tuberculous pleural effusions was studied. The proportion of cells at and beyond the G1 phase of the cell cycle displaying interleukin-2 receptor, transferrin receptor or gp 40/80 glycoprotein in avidin-biotin-peroxidase complex (ABC) staining was 6 +/- 2 percent, 8 +/- 3 percent, and 14 +/- 4 percent of all pleural fluid mononuclear cells, respectively. These findings imply that only a fraction of pleural fluid lymphocytes is activated in tuberculosis. The proportion of autoradiographically visualized 3H-thymidine incorporating blasts at the S phase of the cell cycle was 1.2 +/- 0.3 percent. This phenomenon further confirms the impression that, in spite of activation, most of the pleural fluid mononuclear cells are resting cells. The T4/T8 ratio in the functionally active blast cell population determined by a double labeling method combining ABC staining with autoradiography was similar to the total T4/T8 ratio (2.3 +/- 0.7 vs 2.7 +/- 0.8, p greater than 0.05) calculated for all pleural fluid mononuclear cells.

Humans↗
PubMed1986

Expression of MHC class II antigen, interleukin-2 receptor, transferrin receptor and gp 40/80 glycoprotein during different phases of a normal PHA-driven lymphocyte activation in vitro.

This study characterizes the temporal profile of MHC class II antigen, interleukin-2 receptor, transferrin receptor and gp 40/80 glycoprotein lymphocyte activation markers in relation to each other during different phases of PHA-dependent cellular activation in vitro. Binding of these monoclonal lymphocyte activation-probes was visualized by the avidin-biotin-peroxidase complex method. Maximum PHA-dependent MHC class II antigen expression of 27 +/- 3% was observed on culture day 1, but later no significant differences were observed in MHC class II antigen expression between PHA-driven or culture media-containing control cultures. On the contrary, interleukin-2 receptor (78 +/- 6%) and transferrin receptor (75 +/- 5%) expression reached a maximum on culture day 3, coinciding with a maximum proliferative response. On culture day 5, when 3H-thymidine incorporation was already on the decline, gp 40/80 glycoprotein reached a maximum PHA-dependent expression of 78 +/- 2%, which differed significantly from interleukin-2 receptor (60 +/- 8%, p less than 0.05) and transferrin receptor (51 +/- 8%, p less than 0.01) expression. This study suggests that MHC class II antigen, interleukin-2 receptor, transferrin receptor and gp 40/80 glycoprotein, although all of them are lymphocyte-activation markers, differ as to the chronological sequence of their appearance and disappearance. Their combined use in lymphocyte-activation marker profile assay therefore gives valuable information about the lymphocyte activation state.

Antigens, Surface↗
PubMed1984

Immunocompetent and accessory cells in dermatitis herpetiformis.

Monoclonal antibodies were used in conjunction with the biotin-avidin immunolectin method and the indirect immunofluorescence method to detect lymphocyte subsets in patients with dermatitis herpetiformis (DH) in 50% potassium iodide (KI)-induced skin lesions and in density-gradient-isolated peripheral blood. The proportions of T3-, T4-, and T8-positive lymphocytes in peripheral blood in patients with DH were 76 +/- 6, 48 +/- 7, and 28 +/- 4% and did not differ significantly from those in healthy controls. Among the inflammatory cells in situ in the reticular dermis, 82 +/- 5% were T3-positive lymphocytes, indicating a T-lymphocyte dominance in mature KI-induced DH lesions. The difference in the proportion of T4-positive lymphocytes in mature DH skin lesions and in patient blood was significant (63 +/- 9 and 48 +/- 7%, respectively (P less than 0.001), indicating that the composition of the dermal infiltrate does not reflect nonselective entrapment of blood mononuclear cells in situ. Studies of the kinetics of the local inflammatory response disclosed that at the preblister stage endogenous peroxidase-positive cells and granular, extracellular peroxidase were observed in the dermal papillae only 6 hr after induction, suggesting locally released proteinases as possible complement-degrading factors. Second, at the same time 63 +/- 15% of the inflammatory round cells in the dermis were Ia-positive, endogenous peroxidase-negative, T3- and T6-negative cells, indicating a pathomorphogenetic role for an early event no longer observable in the mature DH skin lesion.

Dermatitis Herpetiformis↗
PubMed1984

Immunohistochemical and electron microscopic characterization of the cellular infiltrate in alopecia (areata, totalis, and universalis).

The inflammatory cell infiltrates in scalp skin of 35 patients, 20 with alopecia areata (AA), 7 with totalis, and 8 with universalis were characterized with the ANAE (alpha-naphthylacetate esterase) marker, monoclonal antibodies, and electron microscopy. As demonstrated by the ANAE staining, no clear difference in the main lymphocyte subclasses (T and B cells) or macrophages was seen between the different types of alopecia or as compared to control patients' scalp skin. However, T lymphocytes and macrophages were seen most frequently and in greater numbers perivascularly and infiltrating the hair bulb in those cases of AA where active hair loss took place. Using monoclonal OKT (OKT-3, -4, and -8) antibodies and the avidin-biotin immunoperoxidase method on frozen sections, a concentration of OKT-8 reactive cells (suppressor/cytotoxic T cells) was seen peribulbarly and invading the hair infundibulum. The cells affecting the hair infundibulum were further studied by electron microscopy. They could be classified into three main types: small lymphocytes (60%), macrophages (30%) and cells closely resembling large granular lymphocytes (LGL) (10%). LGL have previously been considered to be human natural killer (HNK) cells. Thus the hair follicle seems to be the target for the cellular immune response in alopecia. Whether HNK cells participate in the destruction of hair bulbs remains to be investigated.

Adolescent↗
PubMed1984

T lymphocyte subclasses in rheumatoid synovia as analysed with monoclonal antibodies and functional in vitro tests.

Inflammatory synovial T lymphocytes were released by mincing rheumatoid arthritis (RA) affected synovium, by digesting the material with collagenase plus DNAse, and isolating by sheep red cell rosetting and density centrifugation. There was a wide variation in the T-helper (Tm, OKT4) and T suppressor (Tg, OKT8) lymphocyte ratio in the individual synovia, ranging from 0.55 to 1.57. The mean ratio of Tm/Tg lymphocytes as well as OKT4/OKT8 lymphocytes was somewhat lower in the synovium (and in the blood) of RA patients than in the blood of healthy persons, but the differences were not significant (p = 0.56 and 0.09, respectively). The helper and suppressive capacity of synovial T lymphocytes on T-dependent B-cell maturation to immunoglobulin synthesis was analysed by co-culturing them with normal B or (unseparated) T+B cells in the presence of pokeweek mitogen. Eluates where the helper/suppressor ratio was above 1.2 produced at least some T cell help and lacked suppressor capacity, whereas eluates with a T helper/suppressor ratio below 1.20 provided a strong suppression and lacked the capacity of T-cell help. On the whole, we were unable to demonstrate any uniform pattern of inflammation with regard to T-cell subsets in the rheumatoid synovium. However, it seems that monoclonal antibodies provide good markers with which to analyse the inflammatory T cells in situ, and that these markers correlate well to the functional capacity of these cells in vitro.

Adult↗
PubMed1983

Inflammatory cells in sarcoid granulomas detected by monoclonal antibodies and an esterase technique.

Inflammatory cells in situ in Kveim reaction papules were identified in 15 patients with avidin-biotin-peroxidase complex and biotin-avidin-peroxidase methods for surface epitopes and with a simultaneously capturing azo dye method for cytoplasmic acid alpha-naphthyl acetate esterase (ANAE). The spatial relationship of cells in granulomas indicates a concentric arrangement. Endogenous peroxidase-negative, ANAE+, but OKIa-negative immunoincompetent epithelioid cells were in the center. T3+, ANAE+ T lymphocytes formed 60-80% of all cells in the lymphocyte mantle surrounding the epithelioid core. T4/T8 was 2:1. Equal proportions (5-15%) of Ia+ lymphocytes and M pattern ANAE+, endogenous peroxidase-positive mononuclear phagocytes on the one hand and T3+ and T pattern ANAE+ cells on the other in individual patients indicate that the proportion of activated T blasts in situ was less than 15%. The close contacts between different immunocompetent cells in the periphery indicates this as the site for cellular interactions.

Adult↗
PubMed1982

Skin response to intradermal DNA and RNA in systemic lupus erythematosus.

The local response of 18 patients with active systemic lupus erythematosus to 0.1 ml of intradermally injected 0.1% polymerized calf thymus DNA and synthetic double-stranded polyinosinic-polycytidylic acid was studied. In 14 patients positive for DNA, 61 +/- 8% of the inflammatory cells in the subepidermis at 24 hours were acid alpha-naphthyl acetate esterase-positive T lymphocytes. A leukocytoclastic vasculitis was observed in the deeper dermis. Rheumatoid arthritis patients and acne patients had negative responses. These results indicated an abnormal cellular and humoral in vivo response by patients with systemic lupus to DNA. It is suggested that the epidermal Langerhans cells were responsible for the topographic dichotomy of the local DNA response. Test results were positive for polyinosinic-polycytidylic acid in 12 patients, for DNA in 14 patients, and for both in 9 patients. In the 9 patients with positive results for both tests, comparison of responses to each test indicated that the reaction intensity was dependent on the patient and not on the type of polynucleotide acid that was injected.

DNA↗
PubMed1982

Fixation-dependent cytoplasmic false-positive staining with an immunoperoxidase method.

Fixation-dependent nonspecific staining with the unlabeled immunoperoxidase (PAP) method was studied using paraffin-embedded human spleen sections fixed in various fixatives; the specific primary antiserum was omitted or nonimmunized normal rabbit serum was used. Strong cytoplasmic staining of polymorphonuclear leucocytes and macrophages was found after fixation in acetone, alcoholic formalin (94% alcohol) and absolute ethanol. This staining was mainly produced by the second layer of the PAP method. The most probable explanation of this phenomenon is nonspecific protein-immunoglobulin interaction as a result of alcoholic or acetone fixation of the sections. The present findings point to the importance of controls for each case under study to avoid false-positive interpretations.

Humans↗
PubMed1982

Oral lesions in lichen planus and systemic lupus erythematosus. A histochemical and immunohistochemical study.

The present study aimed to characterize and compare the inflammatory cells in the oral lesions in lichen planus and systemic lupus erythematosus. The inflammatory cell subtypes were identified by the combined use of morphological criteria and intracellular markers. In lichen planus 70-90% of all lymphocytes in a band-like submucosal infiltrate were acid alpha-naphthyl acetate esterase (= ANAE)-positive T lymphocytes. The lymphocyte band was sandwiched between mature tissue macrophages and plasma cells. In systemic lupus erythematosus (SLE) the lymphoid cell infiltrate pattern was more variable with extension of the infiltrate to the deeper connective layers and with a more patchy arrangement. The number of ANAE-negative (B) lymphocytes equalled the numbers of ANAE-positive T lymphocytes. In addition, large numbers of plasma cells were present in some lupus patients. In both diseases IgG was produced by 80% of all plasma cells in situ and there were equal portions of cells containing kappa and lambda light chains.

Histocytochemistry↗