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Biomedical subjects

E Trybała

Publications and source records attributed to E Trybała.

9 recordsLinked to original sources
PubMed1993

An intradermal test for the diagnosis of BHV-1 infection. The effect of repeated testing on the immune status of cattle.

The effect of the repeated delayed-type hypersensitivity (DTH) testing (an intradermal test--ID) on virus neutralizing (VN) antibody and DTH responses has been studied. Repeated intradermal injection of inactivated BHV-1 antigen elicited neither the virus neutralizing antibody nor positive skin reaction in cattle free from BHV-1 infection. On the other, hand, the same manipulation carried out in a herd infected with BHV-1 provoked seroconversion and/or positive DTH reaction in some of the animals without detectable pre-existing VN antibody. Cattle initially positive by VN and intradermal tests showed steady increase in antibody titre, and some of them developed little or no skin reaction following repeated DTH testing.

Animals↗
PubMed1993

An evaluation of an intradermal test for the diagnosis of bovine herpesvirus type 1 (BHV-1) infection in cattle.

An intradermal (ID) test (a delayed-type hypersensitivity test) was used for the diagnosis of BHV-1 infection in cattle. A threshold value for the test positive results was established based on the analysis of the agreement between the results of ID test and those of virus neutralization (VN24) assay. Linear regression analysis revealed no correlation between the intensity of the skin reaction (expressed as an increase in skin-fold thickness) and the VN24 antibody titre. The sensitivity and specificity of the ID test compared to VN24 one, were 86.6% and 98.3% respectively. Of the 487 cattle with an age of over 6 months, 25 (5.1%) reacted discordantly by ID and VN24 tests, i.e. 19 seropositive animals expressed little or no skin reaction, whereas 6 seronegative individuals were positive by ID. Neither of the calves, with passively acquired antibodies in contrast to experimentally infected ones, showed distinctive skin reaction.

Animals↗
PubMed1992

[Is Aujeszky's disease a zoonosis?].

Several cases of human illness with symptoms suggestive of Aujeszky's Disease (AD) at an outbreak of the disease in cattle and swine were evaluated during this study. Additionally serological studies of people in the group at high risk for illness caused by Aujeszky's Disease Virus (ADV) were carried out. In a herd of 180 bulls sudden clinical disease occurred with symptoms of muscular tremors, salivation, sweating and severe pruritus of the head. The clinical disease lasted two to eight hours and finished in death. In 7 days 10 bulls died and 60 were either killed or culled. A biological test for AD was positive. ADV was identified in the brain of a dead bull. Serological studies for the bulls were negative, however 92.9% on the premises farm were asymptomatically infected. On third to the fifth day of disease in the cattle clinical signs appeared in six of seven workers which had direct contact with diseased cattle. A pruritus of the palms, which spread onto the lower and upper arms, shoulders and back lasted several days.

Adult↗
PubMed1991

The effect of some physical and serological treatments on haemofusing activity of bovine parainfluenza virus type 3.

Bovine parainfluenza virus type 3 irrespective of the time of its harvesting from Madin-Darby bovine kidney cells, expressed little or no haemofusing activity. Treatment of the virus by freezing and thawing, sonication or antibody and complement enhanced this activity. Moreover haemofusing activity did not correlate with the viral capacity for fusion of susceptible cells in monolayer cultures.

Animals↗
PubMed1990

Hemagglutination by herpes simplex virus type 1.

The McIntyre and HSZP strains as well as clinical isolate of herpes simplex virus type 1 were found to agglutinate C57Bl/10su and CBA mouse red blood cells. The hemagglutinating activity was inhibited by antisera that neutralized the infectivity of the virus.

Animals↗
PubMed1989

[Effect of urea and uric acid on the hemolytic activity of Sendai virus].

It was found that haemolytic activity of Fushimi strain of Sendai virus multiplied in allantoic cavity of chicken embryos is independent on its haemagglutinating titer and also on allantoic fluid urea and uric acid content. It was shown in experiments with embryonated eggs that these two compounds have no also influence on haemolytic activity induction in Sendai virus. Moreover, the results of an experiment in which allantoic fluid was replaced by Eagle's liquid suggest that most probably the other components present in allantoic fluid do not also influence the appearance of haemolytic activity of this virus.

Animals↗
PubMed1989

An evaluation of four serological tests for the detection of antibodies to bovine parainfluenza virus type 3.

Haemagglutination-inhibition (HI), virus neutralization (VN), haemolysis-inhibition (HLI-1, HLI-2) tests, and two new haemofusion-inhibition (HFI-1, HFI-2) tests, developed by us, were tested for the detection of antibodies to bovine parainfluenza virus type 3 (BPIV-3) in sera of 45 steers randomly selected from a herd naturally infected by BPIV-3. Twelve seronegative animals were then vaccinated with formalin-inactivated vaccine and 15 days later tested by the above-mentioned assays. Linear regression analysis revealed positive correlations between HI, VN, HLI-1 and HFI-1 antibody titres, which confirm the specificity of the HFI-1 test. The HLI-1 and HFI-1 assays proved to be less sensitive than the HI and VN ones, while HLI-2 and HFI-2 tests failed to detect any antibody to BPIV-3.

Animals↗
PubMed1987

The effect of delayed harvesting as well as freezing and thawing on biological properties of Newcastle disease virus.

Delayed harvest of Newcastle disease virus (NDV) from eggs was less infective (as expressed by its lower infectious titer and a higher number of virus particles per EID50) and more resistant to a temperature of 50 degrees C than the early harvest. On the other hand, little or no effect of delayed harvesting was found on neuraminidase, erythrocyte-fusing and immunogenic properties. Moreover, haemolytic activity of NDV was moderately enhanced when its harvesting from de-embryonated egg was delayed. Treatment of a fresh NDV preparation with repeated freezing and thawing cycles also caused a marked reduction in virus infectivity and induction of its haemolytic activity.

Animals↗