Biomedical subjects

E Tsoulou

Publications and source records attributed to E Tsoulou.

4 recordsLinked to original sources

PubMed2003

Changes in DNA flexibility after irradiation with gamma rays and neutrons studied with the perturbed angular correlation method.

Neutron and gamma irradiation of buffered solutions of calf thymus DNA resulted in changes in the dynamics of the macromolecule. In the low-dose region (0.8-10 cGy of 239Pu-Be neutrons and 0.34-3 Gy of 60Co gamma rays), the flexibility of DNA decreased as indicated by slower rotation of the molecules. Neutrons appeared to be approximately 35 times more effective than 60Co gamma rays. The rotational correlation time, tau C, was measured using the perturbed angular correlation (PAC) method. Its variation appears to follow a linear-exponential behavior. An attempt is made to formulate this behavior as a function of the energy deposited on the macromolecule (radiation dose), the average threshold energy (dose) required to form new lesions, and the available population of intact DNA sites.

Cobalt Radioisotopes↗

PubMed2002

Radiosensitivity of V79 cells after alpha particle radiation at low doses.

Low dose hyper-radiosensitivity (HRS) of V79 cells was demonstrated after irradiation with gamma rays and 4He2+ ions of various linear energy transfer (LET) values (58.9, 79.3 and 101.7 keV.micron-1). In parallel, the cytogenetic analysis showed an LET dependence of aberrations at a dose of 1 Gy, while the observed chromatid fragments appeared to vary with the number of 4He2+ ions traversing the cell nucleus. The results of both studies are correlated so as to achieve a better understanding of the so-called induced radioresistance. The cell mechanism of radioresistance appears to be induced after a certain amount of energy is deposited in the cell nucleus. This amount depends both on the radiation quality as well as the number of particles traversing the cell, inducing chromosome alterations and chromatid damage.

Alpha Particles↗

PubMed2001

Low-dose hypersensitivity of V79 cells under exposure to gamma-rays and 4He ions of different energies: survival and chromosome aberrations.

PURPOSE: To examine the low-dose sensitivity of V79 cells under exposure to gamma-rays and 4He ions of different energies. MATERIALS AND METHODS: Cell survival and cytogenetic analysis using the Giemsa technique were studied following irradiation to doses of 0-3 Gy at the INFN-LNL facilities. RESULTS: Low-dose hyper-radiosensitivity (HRS) of V79 was demonstrated after irradiation with gamma-rays and alpha-particles of various linear energy transfers (LET) (58.9, 79.3 and 101.7 keV microm(-1)). Cytogenetic analysis showed an LET dependence of aberrations at a dose of 1Gy; the frequency of chromatid fragments appeared to vary with the number of alpha-particles traversing the cell nucleus. The results of both studies fit together to give a better understanding of so-called 'induced radioresistance' phenomenon. CONCLUSIONS: The mechanism of induced cellular radioresistance appears to be initiated after a certain amount of energy is deposited in the cell nucleus. This amount depends on both radiation quality and the number of particles traversing the cell.

Animals↗

PubMed2001

Probing irradiated DNA with the perturbed angular correlation method.

The technique of perturbed angular correlations of gamma rays has been used to study the effects of radiation on DNA molecules. The samples are buffered solutions of calf thymus DNA exposed to various doses (0-80 Gy) of gamma rays. Indium-111 is used as a probe. Rotational correlation times, tau(c), a parameter measuring the flexibility of a macromolecule, are obtained that show a dependence on radiation dose.

Animals↗