Forty-one patients with sarcoidosis were investigated for cardiac involvement. The results are summarized as follows: The focal left ventricular perfusion defect was found on the TMPS's of 5 out of 41 patients with sarcoidosis. The defect disappeared in four of these five patients over a 3-year period, but remained in the patient with fatal myocardial sarcoidosis. A decreased left ventricular ejection fraction was found in 14 out of 41 patients with sarcoidosis, and in 3 out of 5 patients with the left ventricular perfusion defect. Echocardiographic examination did not reveal left ventricular wall dysfunction at the site of the left ventricular perfusion defect, except in one patient with fatal myocardial sarcoidosis. Holter ECG's showed such abnormalities as CRBBB, heart blocks, PVC, and PSC in 13 out of 32 patients with sarcoidosis. SACE activity was elevated in all three patients with the left ventricular perfusion defect that were examined, and it could be correlated with changes in the left ventricular perfusion defect in one of them.

Adult↗

PubMed1986

Distribution of Gc protein (vitamin D binding protein) on the surfaces of normal human lymphocytes and leukemic lymphocytes.

Distribution of membrane-bound Gc protein (mGc) in normal lymphocyte subpopulations and leukemic lymphocytes was studied using a rabbit antiserum specific to Gc protein. Forty to fifty percent of the normal peripheral blood mononuclear cells were stained with anti-Gc antiserum by membrane immunofluorescence, and almost all of the B cells (B1+) and NK cells (Leu 11+) were mGc-positive. Only a few resting T cells (T3+) reacted with anti-Gc, but mGc appeared in a significant number of the T cells after activation by PHA. The distribution of mGc on leukemic lymphocytes of various types nearly corresponded to that in normal lymphocyte subpopulations.

B-Lymphocytes↗

PubMed1986

Enhanced calcium-sensitivity of erythrocytes in hypertension--calcium-induced changes of erythrocyte osmotic fragility in essential hypertension.

To investigate the Ca-sensitivity of the erythrocyte membrane in hypertension, the changes of the osmotic fragility of erythrocytes by Ca-loading and the effects of Ca-channel blockers or calmodulin-antagonist were observed in patients with essential hypertension. Erythrocytes were obtained from untreated patients with essential hypertension and age-matched normotensive subjects. Treatment of erythrocytes with Ca-ionophore A23187 and Ca in bathing medium caused the reduction of the osmotic fragility of erythrocytes dose-dependently on Ca-concentration. The degree in alteration of the osmotic fragility of erythrocytes was greater in essential hypertension than that in normotensive controls. In addition, Ca-induced changes of erythrocyte osmotic fragility was inversely correlated with the plasma renin activity in essential hypertension. In the presence of Ca-antagonists (verapamil, diltiazem) or calmodulin-antagonist (trifluoperazine), the reduction of the osmotic fragility of erythrocytes by Ca-loading was inhibited, and the differences of the osmotic fragility of erythrocytes between the hypertensives and the normotensive controls were abolished by these drugs. These results suggest that the greater changes of the osmotic fragility of the erythrocytes by Ca-loading in essential hypertension might be due to the abnormality of Ca-handling of the cell membranes causing an increase in the intracellular Ca concentration, contributing at least partially to the pathogenesis of hypertension.

Adult↗

PubMed1986

Leu 11+ T gamma cell chronic lymphocytic leukemia with partially activated natural killer function and its further activation by recombinant IL2 in vitro.

A patient with T gamma cell chronic lymphocytic leukemia with the Leu 11+ phenotype and novel function of activated natural killer cells is reported. The peripheral blood mononuclear cells of this patient showed large granular lymphocytes by May-Giemsa staining and lamellipodia by scanning electron micrography. Tests on reactivity with monoclonal antibodies showed that most cells were Leu 11+, OKT3-/Leu 1-, OKT4-, OKT8-, Leu 7-, OKM1-, and Tac-. Freshly collected cells lysed not only K562, which is highly sensitive to natural killer cells, but also Raji cells and Daudi cells, which are not. Leu 11+ cells were triggered by recombinant interleukin 2 (rIL2) to proliferate, produce gamma-interferon (gamma IFN), and show enhanced HLA-DR antigen expression, and 30% of the Leu 11+ cells became positive for IL2 receptor antigen (Tac). The spectrum of cytotoxic activity of these cells against target cells was extended by rIL2; after treatment with rIL2, the cells also lysed HeLa cells and even fresh cancer cells. This stimulation also increased the activities of acid phosphatase and tartrate-resistant acid phosphatase of the cells and resulted in the appearance of nonspecific esterase activity. The expanded cell population may represent a neoplasm, but these findings provide information on a novel differentiation stage of activated NK cells.

Aged↗

PubMed1985

Association of specific tyrosine phosphorylation with stages of B-cell differentiation in human lymphoid leukemias.

In vitro protein phosphorylation in various types of human fresh lymphoid leukemic cells (C-ALL, B-CLL, HCL and PCL: B-cell lineage and T-ALL, ATL and T-CLL: T-cell lineage) were studied. In cases of B-CLL and HCL, tyrosine protein kinase (TPK) activity was at least 5-fold higher than that in other cases of B- and T-cell lineages. B-cell leukemic cells at various differentiation stages had different endogenous substrates in tyrosine phosphorylation as well as distinct TPK activity. The P-tyr-containing proteins of 68K, 59K and 56K were detected commonly in all the cases of B-cell lineage. The phosphorylated protein of 32K was present only in cases of PCL. On the other hand, in T-ALL and ATL, the major substrate in tyrosine phosphorylation was 58K. These results suggest that the characterization of in vitro tyrosine phosphorylation provides a new means not only to distinguish T- and B-lymphoid leukemia, but also to differentiate stages of lymphoid development.

B-Lymphocytes↗

PubMed1985

[Angiographic evaluation of hyperthermic effect of VX2 rabbit carcinoma].

Explore the source record for details and available documents.

Animals↗

PubMed1985

Serum immunosuppressive acidic protein in adult T-cell leukaemia (ATL).

The availability of serum immunosuppressive acidic protein (IAP) as a marker of subtypes of adult T-cell leukaemia (ATL) was examined. Serum IAP levels were measured in 34 patients with ATL (18 typical, 9 atypical and 7 smoldering), 7 healthy carriers of ATLA antibody and 53 healthy controls. The mean value of serum IAP was significantly higher in patients with typical ATL (897.8 +/- 502.4 micrograms/ml) than in those with atypical ATL (426.7 +/- 106.6 micrograms/l), smoldering ATL (310.0 +/- 51.3 micrograms/ml), healthy carriers of ATLA antibody (302.9 +/- 39.5 micrograms/ml) and normal controls (350.5 +/- 73.2 micrograms/ml). Serial determinations of IAP revealed that the level was correlated with the clinical course in patients with ATL; there was a difference in the prognosis of patients with high and normal levels of IAP (P less than 0.05). Thus, routine measurement of serum IAP seems useful in differentiating typical, atypical and smoldering ATL and also in evaluating the prognosis of patients.

Adult↗

PubMed1985

[Mitral regurgitation: detection and quantitative evaluation by two-dimensional Doppler echocardiography].

Mitral regurgitation was detected and quantitatively evaluated using two-dimensional Doppler-echocardiography. The subjects consisted of 74 cases having a variety of heart diseases, who underwent left ventriculography within one week before or after the Doppler study. Among 50 patients with mitral regurgitation confirmed by left ventriculography, the Doppler study detected mitral regurgitant flow signals in 46, for a sensitivity of 92%. Minimal mitral regurgitation in four cases could not be detected by Doppler studies. Twenty-four patients had no mitral regurgitation according to left ventriculography; all but one also had no mitral regurgitation by Doppler study, for a specificity of 96%. In one false positive case, typical mitral regurgitant flow signals were detected in an area localized within the left atrial cavity near the mitral valve orifice. The possibility remains that left ventriculography missed this minimal regurgitation. For quantitative assessment of mitral regurgitation, the following two methods were used. Three long-axis views through the lateral, middle and medial parts of the mitral valve, and a short-axis view at the level of the mitral orifice were imaged via the parasternal approach. The area where mitral regurgitant flow signals were detected was mapped on each cross-sectional echocardiogram, then the distance attained by the regurgitant flow from the mitral valve and the area covered by the regurgitant flow were determined. The maximal distance among the three long-axis views and the sum of the distances in these views was parallel to the severity of mitral regurgitation as assessed by left ventriculography.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1985

[Two-dimensional echocardiography in diagnosing the region of myocardial infarction: a comparative study by several independent examiners].

The efficacy of two-dimensional echocardiography in diagnosing the localization of myocardial infarction (MI) was studied by comparing the echocardiographic and pathological findings of 28 patients having MI. The ventricular wall was divided into 18 segments including three segments of the right ventricular wall. The regional wall motion abnormalities for each of the 504 segments were diagnosed by visual assessment. The echocardiographic recordings were reviewed individually by four examiners using the same protocol to assess the interobserver's variation. The receiver operating characteristics (ROC) curves differed by examiners. However, when the subjects were limited to anterior MI patients, the ROC curves established by the echo-trained physicians did not differ significantly. It was concluded that the echocardiographic diagnosis of regional wall motion by visual assessment has universal validity. Individual differences are thought mainly to depend on the sites of infarction. Sensitivities for detecting transmural (TM), non-transmural (non-TM) infarcted segments and intact segments were 90, 70 and 70%, respectively. Most of the underestimated TM or overestimated intact segments corresponded to the sites adjacent to MI. As the unexpectedly misdiagnosed segments, the mimic inward motion of the inferior wall drawn by the intact anteroapical wall was observed in a inferior MI patient, or the mimic anterior motion of the anteroapical wall by a swinging motion of the heart, or the paradoxical motion of the interventricular septum was observed in a right ventricular MI case. In the two third of the overestimated non-TM segments, it was considered that the wall motion was affected by the myocardial ischemia, which was not revealed by pathological examination. The underestimated non-TM segments located adjacent to the intact segments or opposite to the severely ischemic segments. From the echocardiographic viewpoint, nearly 90% of segments showing akinetic or dyskinetic motion had MI. However, one third of segments diagnosed as normal were actually MI segments.

Aged↗

PubMed1985