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Biomedical subjects

E V Egorova

Publications and source records attributed to E V Egorova.

At least 55 records · Page 3Linked to original sources
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[Oxidation of pyridine nucleotides in injuries of the cornea].

NADH fluorescence may serve a convenient test indicating the tissue redox status. Rabbit cornea has been incubated at ambient temperature in media of different compositions with 5.10(-5) M NADH at pH 7.0-7.3. Oxidation of NADH in the humor-substituting medium for corneal tissue incubation has been monitored by fluorescent analysis (stimulation maximum at a wavelength of 360 nm, emission maximum 460 nm). Characteristic kinetics of changes in the pyridine nucleotide fluorescence consists in its maximum intensity occurring in 10 min of the tissue incubation, followed by the fluorescence intensity reduction by the 20-30th min of the corneal tissue incubation. These changes are explained by a release of substrates, characterized by NADH oxidizing activity, from the corneal tissue, this resulting from injury to the corneal cells with humor-substituting solutions. Carnosine (10 mM) and taurine (0.05% solutions) have been found to protect the corneal tissue from injuries, in contrast to common salines.

Animals↗
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[Spectral characteristics of intraocular lenses and injury to the retina caused by visible light].

It has been shown that intraocular lenses (IOL) prepared from polymethylmethacrylate and silicon let pass the light of short-wave visible and long-wave spectrum regions. Unlike natural lens, IOL have no yellowish coloration, which results in the appearance of chromatic aberrations and brightness of IOL during implantation. Mechanisms of damaging effects of visible light on the retina were studied. It has been shown that a week after the rat eyes were illuminated the waves "a" and "b" ERG were inhibited. At the ultrastructural level damage of the cells of the retinal pigment epithelium and of the retinal layers formed by photoreceptor cells took place. Dynamics of the damage of tissue structures of the retina by visible light was investigated. It is suggested that application of colored lenses and prophylaxis by the inhibitors of free radical oxidation will protect the retina from photochemical damage.

Animals↗