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Biomedical subjects

E V Eretskaia

Publications and source records attributed to E V Eretskaia.

9 recordsLinked to original sources
PubMed1989

[Experimental substantiation of the early use of applied sorption in treating wounds].

In the experiments on rabbits with bilateral musculocutaneous wound of a thigh, it is shown that the early use of the "Dnepr MN" activated carbon fibrous material (ACFM) intended for medical purposes provides timely haemostasis in the damaged tissues, reduces blood loss, prevents the development of traumatic edema, decreases the intensity of inflammatory process in a wound, facilitates rapid and uncomplicated healing of wounds under the artificial crust. The treatment-and-prophylactic effectiveness of ACFM is associated with its ability for rapid and irreversible sorption of biologically active substances forming in the wound tissues, and as well with protection of a wound against secondary infection.

Absorption↗
PubMed1989

[Effect of applicative-sorptive therapy on the activity of various enzymes and inhibitors of the kallikrein-kinin system in traumatic disease].

On the model of musculocutaneous wound in rabbits, the effect of applicative sorption with the use of activated carbonic fibrous materials on the course of traumatic disease was studied. The total esterase activity of the blood serum, activity of alpha 1-inhibitor of proteinases, kallikrein content in the blood serum were defined. It is shown, that changes in biochemical indices are in agreement with the findings of clinical observations and outcome of traumatic disease. The use of activated carbonic fibrous material at the early period of traumatic disease contributes to its favourable course, prevents the development of complications and death of the animals from sepsis.

Animals↗
PubMedDate not supplied

[Activation of plasminogen in its immobilization].

Plasminogen is established to be able of transforming into the plasmin-like is compound when it is immobilized on aminoethyl cellulose by means of glutaric aldehyde. The plasminogen immobilized in such a way manifests a caseinolytic activity and is not activated additionally by streptokinase. The plasminogen immobilized on bromocyanogen-activated sepharose and cellulose, like soluble proenzyme, has no activity of plasmin and retains the ability of being activated with streptokinase in catalytic amounts.

Caseins↗
PubMedDate not supplied

[Activation of plasminogen with immobilized trypsin].

Human plasminogen isolated from the placenta serum fraction by means of affinity chromatography was activated by trypsin being in covalent bond with sepharose. The activation is studied as dependent on pH, temperature and the proenzyme-activator ratio in the presence of 25% glycerol as a stabilizing agent and without it. Utilization of the immobilized trypsin as a plasminogen activator makes it possible to transform completely the proenzyme to plasmin varying the plasminogen-trypsin ratio and time of activation when it is conducted under optimal conditions: in the presence of 25% glycerol at pH 7.0-7.1 and the temperature of 30 degrees C.

Enzyme Activation↗
PubMedDate not supplied

[Affinity sorbents for obtaining plasminogen].

Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons.

Chromatography, Affinity↗
PubMedDate not supplied

[The effect of application-sorption therapy on dynamics of activity of molecular carriers of electrons of the energetic and detoxifying systems of cells in experimental burn disease].

Integral free radical state, paramagnetic enzyme complexes of nonheme iron with sulfur- and nitrogen-containing ligands in mitochondria and heme iron in microsomes as well as metalloenzymes of blood plasma ceruloplasmin and transferrin were studied in dynamics of burn disease in rats. Significant decrease in content of reduced ferrosulfur proteins in mitochondrial energy system was observed in kidneys within the second day after the burns and in liver tissue within the 14-21 days after the failure which correlated with reduction of cytochrome P-450 oxidized form. Development of paramagnetic nitrosyl complexes of heme and nonheme iron, specific for hypoxia, were detected in the impaired muscle tissue. Increase in tissue hypoxia was accompanied by decrease in content of transferrin and by elevation in the antioxidant activity of blood plasma. Application of local adsorption after early excision of burns crust contributed to normalization of both metabolism in the impaired tissues and in activity of the electron transport chain in liver and kidney tissues. Therefore, local adsorption therapy may be considered as an alternative procedure in sorptional detoxication of burns.

Adsorption↗