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E V Salina

Publications and source records attributed to E V Salina.

8 recordsLinked to original sources
PubMed2003

[A method for measuring the strength of adhesion of Candida albicans on epithelial cells].

A method on estimation of Candida albicans adhesion to epitheliocytes is proposed. The principle consists in the fact that while centrifuging epitheliocytes (1000 cycles/min, 10 min) with adhered Candida albicans through phycoll-verografin (1.077 g/cm3 density), Candida albicans cells having poor adhesion are separated from epitheliocytes trying to rise to the surface of phycoll-verografin. Candida albicans strongly adhered to epitheliocytes settle down to the bottom of test tube. In experiments with buccal epitheliocytes of healthy donors the number of strongly adhered Candida albicans ranged from 13% to 50% and they amounted to 37.0 +/- 7.8% on an average.

Candida albicans↗
PubMed1983

[Reactivity to Staphylococcus aureus peptidoglycan in a neutrophil phagocytosis system].

The mechanisms of the formation of reactivity in the system "S. aureus peptidoglycan--serum--neutrophils" were studied. The direct interaction of peptidoglycans with neutrophils isolated from human blood was not accompanied by their stimulation, evaluated by an increase in the reduction of nitro blue tetrazolium. Antibodies to peptidoglycan determined the final result of the opsonic activity of normal serum: the IgG fraction isolated from commercial immunoglobulin was active at a concentration of 1 mg/ml. The activity of complement was considerably lower: it was manifested after the removal of antibodies and in cooperation with suboptimal dosage. The IgG-independent activation of complement was equally blocked by 10 mM EDTA and Mg-EGTA. Residual activity (after the removal of antibodies an the blocking of complement) constituted an insignificant proportion of the total opsonic reserve of the serum. The specific features of the stimulating activity of peptidoglycans obtained from different S. aureus strains were observed.

Antibodies, Bacterial↗
PubMedDate not supplied

[Phenomenon of selective decrease of colonization (adherence) resistance in the system "Candida albicans-buccal epithelial cells"].

In the period of the exacerbation of bronchial asthma an increased adhesiveness (in vitro) of buccal epithelial cells to C. albicans was noted in most children under study (94.7%). This phenomenon was not observed in children with the exacerbation of gastroduodenitis. The characteristics of natural bacterial colonization of buccal epithelium were equally decreased in both groups of patients. These results are regarded as the consequence of the reactive involvement of the epithelium of mucoid tract in the processes destabilizing homeostasis.

Adolescent↗