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Biomedical subjects

E Vendrely

Publications and source records attributed to E Vendrely.

At least 19 recordsLinked to original sources
PubMed1993

Production, characterization, and immunocytochemical applications of monoclonal antibodies to human sperm protamines.

Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for the protamine P1 family, another was specific for the protamine P2 family and the third was common to both families. All were specific for the human species. Antibodies were used to detect protamines in germ cells by indirect immunofluorescence and by immunoelectron microscopy. Protamines appeared in spermtid nuclei at steps 4-5 of spermiogenesis, i.e., during the chromatin condensation process, and were not accumulated in the cytoplasm before entering the nucleus.

Animals↗
PubMed1988

Quantitative ultrastructural analysis of the principal cells in the human epididymis.

A morphometric study of the principal epithelial cells of the epididymis has been carried out in six young adult men. Samples were taken in the head, body and tail of the epididymis and processed for ultrastructural study. The cytoplasm was subdivided into four zones. Their section areas were measured by planimetry and the volume density of cytoplasmic organelles assessed using the point-counting method. The general organization of the principal cells of the epididymis was identical along the duct. Quantitative analysis revealed that cells of the head contained significantly larger amounts of coated-vesicles and of Golgi saccules than the body and the tail. The functional implications of these results is important for the understanding of receptor-mediated endocytosis and protein synthesis in the different parts of the human epididymal duct.

Adult↗
PubMed1986

Gonadotropin responses to low dose pulsatile administration of GnRH in a case of anosmia with hypogonadotropic hypogonadism associated with gonadal dysgenesis 47 XXY.

A 25 year old man presented hypogonadotropic hypogonadism with complete anosmia (Kallman's syndrome). His chromosomic type was 47 XXY (Klinefelter's syndrome). Clinical findings were: height 183 cm, weight 62 kg, increased length of lower limbs, P2-A2 pilosity and micropenis. Only a left testis was present (1.5-1.5 cm). Bone age was 15. Testicular biopsy showed that the signs were more related to the gonadotropic deficit than to the gonadal dysgenesis; tubular hyalinization was not observed. Plasma levels of testosterone and oestradiol were very low. Plasma gonadotropin levels were below normal ranges and did not respond to an infusion test of GnRH. GnRH was administered iv every 90 min for 3 weeks by an auto syringe infusion pump and induced a pulsatile response of FSH and LH. Plasma levels of testosterone and oestradiol were unaffected. It may be concluded that the results of pulsatile injection of GnRH confirmed in this patient a unique association of Kallmann's syndrome with complete 47 XXY Klinefelter's syndrome.

Adult↗