Reconstitution of a thymus cell-deprived immune system by syngeneic and allogeneic thymocytes in vitro.
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Biomedical subjects
Publications and source records attributed to E Wecker.
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Evidence is presented that poliovirus particles with a single lethal hit by hydroxylamine do not induce in host cells either inhibition of cellular protein synthesis or viral ribonucleic acid (RNA) replication. The RNA of these viruses is not replicated even if the cells are simultaneously infected with both active and inactivated viruses. The damaged viral RNA seems to have lost both its template function and its function in the translation of normal viral proteins.
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DNA transfection in mouse L-cells was performed by means of the electropermeabilization technique (U. Zimmermann, G. Pilwat, and F. Riemann, Z. Naturforsch. 29, 304 (1974)). The plasmid pSV 2-neo used leads to neomycin-resistance in stably transfected L-cells. Optimized conditions resulted in high yields of clones at relatively low DNA concentration. The influence of temperature during pulse application and during the subsequent resealing process as well as the field parameters and medium composition are discussed.