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Biomedical subjects

E Wieczorek

Publications and source records attributed to E Wieczorek.

At least 19 recordsLinked to original sources
PubMed1994

Effect of crowding on corticosterone responses to central adrenergic stimulation.

The social stress of crowding for 3, 7 and 14 days considerably reduced the increase in serum corticosterone elicited by intracerebroventricular administration of isoprenaline, a beta-adrenergic agonist, on the 3rd and 7th days of crowding. The corticosterone response to clonidine, an alpha 2-adrenergic agonist, was significantly diminished only after 3 days of crowding and this reduction was paralleled by a significant decrease in hypothalamic histamine content. The stimulatory effect of phenylephrine, an alpha 1-adrenergic agonist, was not significantly changed by crowding stress. Social crowding stress caused almost total and persistent reduction in the hypothalamic-pituitary-adrenocortical (HPA) responsiveness to noradrenaline which stimulates the HPA axis via both alpha- and beta-adrenergic receptors.

Animals↗
PubMed1994

Plasma ANP and cyclic GMP levels versus left ventricular performance at different AV delays in AV sequential pacing.

Eleven resting patients with an implanted DDD pacemaker were studied. After 30 minutes of AV sequential pacing at a rate of 80 beats/min with three consecutive atrioventricular delays (AVDs; 100, 150, and 200 msec) peripheral venous blood was drawn for further analyses by specific radioimmunoassays of atrial natriuretic peptide (ANP) and the ANP second messenger, cyclic guanosine monophosphate (cGMP). Relative changes in left ventricular (LV) stroke volume following alterations of AVD were assessed by means of pulsed-Doppler echocardiography through measurement of LV outflow time-velocity integrals (TVI). The optimal AVD (oAVD) was defined in individual patients as that which was associated with the greatest TVI and with improvement over both other AVDs of more than 4%. The oAVD was found in nine patients. For these nine patients no significant differences in either plasma ANP or cGMP between various AVDs were observed. However, we found such differences with respect to values measured at oAVD; both ANP and cGMP levels were lowest at oAVD. Pooling together the data obtained in 11 patients at three AVDs, a positive correlation between ANP and cGMP levels was found (r = 0.7, P < 0.0001, n = 33). Moreover, changes of plasma ANP and cGMP induced by every AVD increment of 50 msec were also correlated (r = 0.6, P < 0.01, n = 22). It is concluded that in AV sequential pacing at rest plasma ANP reaches minimal levels at the AVD, which provides the best LV performance. Although levels of cGMP changed in parallel with those of ANP, low relative values of cGMP differences may limit the usefulness of cGMP assays in optimization of the AVD.

Adult↗
PubMed1993

[Dynamics of the course of idiopathic juvenile osteoporosis].

Clinical course of the idiopathic juvenile osteoporosis (IJO) was monitored in the group of 45 patients of both sexes with diagnosed disease, verified during follow-up period. The aim of the study was to evaluate the relationship between clinical symptoms and the results of biochemical, anthropometric, and densitometric measurements. An analysis of the obtained data enabled to distinguish the acute and chronic IJO phases. Evolution of the acute phase into chronic one was manifested by the cessation of pain and pathological gait stereotype, normalization of muscular strength, anthropometric parameters and urinary Pyr and DPyr excretion, as well as improvement in bone density. Hypercalciuria and increased urinary excretion of Pyr and DPyr, observed in the acute phase of IJO, may indicate that bone resorption exceeded bone formation. Tendency to maintain of alkaline phosphatase activity within lower limits of the normal values with slight increase during an improvement of densitometric parameters suggested transient osteoblast dysfunction.

Adolescent↗
PubMed1991

Conformational change of the haemolymph juvenile-hormone-binding protein from Galleria mellonella (L).

A juvenile-hormone-binding protein (juvenile-hormone carrier), isolated from Galleria mellonella haemolymph, was treated with trypsin, chymotrypsin, carboxypeptidase A and subtilisin. Among these enzymes, only subtilisin was able to affect juvenile-hormone-binding activity of this protein. With SDS/PAGE it was shown that juvenile-hormone-binding protein, a 32-kDa peptide, is first slowly converted into a 30-kDa molecule, then into two or three smaller-molecular-mass species (20-25 kDa), which in turn were further digested to small peptides undetectable in PAGE. The 30-kDa peptide has a 2.4-times-higher dissociation constant for juvenile hormone than the native protein. No binding activity was detected for 20-25-kDa peptides. The rate of proteolysis of juvenile-hormone-binding protein was decreased by more than twofold in the presence of hormone, however, the overall cleavage pattern was unchanged. Under non-denaturing conditions, free binding-protein molecules could be separated from juvenile-hormone-binding-protein complex due to a slower electrophoretic mobility of the complex. As judged from ultracentrifugation and cross-linking experiments, binding of the hormone to its haemolymph carrier does not induce formation of oligomers, but shifts the sedimentation coefficient from 2.30S to 2.71S. It is concluded that juvenile-hormone binding induces a conformational transition of its carrier protein. This hormone-induced change might have a physiological significance for signal transmission.

Animals↗
PubMed1991

Extremely low serum pyridoxal 5'-phosphate in children with familial hypophosphatemic rickets.

The apparent vitamin B-6 status of 31 children with familial hypophosphatemic rickets (FHR) was determined. All children had alkaline phosphatase activity that was high-normal to elevated for their ages. A sensitive assay for pyridoxal 5'-phosphate (PLP) indicated that 15 of the 31 children had an undetectable (less than 0.2 nmol/L) concentration of the vitamer--the lowest values yet reported in human serum. The 16 remaining children had concentrations of the vitamer so low that they indicated a potential severe vitamin B-6 deficiency. However, none of the children had ever presented with any of the classical vitamin B-6-deficiency symptoms. Treatment of three additional FHR children with 100 mg pyridoxine.HCl/d resulted in a moderate and transient elevation of their serum PLP concentrations, a dramatic elevation of their erythrocyte PLP concentrations, and no improvement in clinical condition. Serum or plasma PLP concentrations are an inappropriate index for determining vitamin B-6 status in people with FHR and perhaps in others with elevated alkaline phosphatase activity.

Adolescent↗
PubMed1991

Circulating immune complexes in Waldeyer's ring hypertrophy.

The investigations on the occurrence of CIC were carried out in 65 children with chronic tonsillitis, 39 with hypertrophy of pharyngeal tonsil and 14 children with chronic tonsillitis and hypertrophy of pharyngeal tonsil. Immune complexes were found in 20 children with chronic tonsillitis and in 5 children with chronic tonsillitis and hypertrophy of pharyngeal tonsil. In the control group (20 healthy children) and in children with hypertrophy of pharyngeal tonsil immune complexes are not detected. Screening of CIC in the period from 1 to 3 months after tonsillectomy or adenotonsillectomy showed disappearance of CIC determined by PEG precipitation method.

Adenoidectomy↗
PubMed1991

Molecular mechanism of the juvenile hormone action.

Structures, physiological role and level regulation of the juvenile hormones are described. A scheme of juvenile hormone mode of action at the molecular level, which includes transport of hormone via its binding protein, is presented.

Animals↗
PubMed1989

Correction of the abnormal ratio of OKT4+ to OKT8+ peripheral blood lymphocytes in patients suffering from juvenile arthritis (JCA) by the extract from calf thymuses.

Children suffering from JCA showed an elevated percentage of OKT4+ T lymphocytes and an increased ratio of OKT4+ to OKT8+ lymphocytes. Several children showing a significant aggravation of the disease, were selected for a 3-week treatment with TFX. In 7 out of 10 patients a pronounced clinical and laboratory improvement was registered, accompanied by a normalization of the OKT4+ to OKT8+ lymphocyte ratio.

Adolescent↗
PubMed1989

[Circulating immune complexes in children with uveitis].

The occurrence of circulating immunological complexes was investigated in 25 children with uveitis (11 with an anterior and 14 with posterior uveitis). The presence of complexes was found in 15 children, among them in 6 with anterior and 9 with posterior uveitis. Besides the diseased children showed a significant lowering of the percentage of the T lymphocytes by simultaneous increased percentage of B lymphocytes and also a raised concentration of the serum immunoglobulins of M, A and G class.

Antigen-Antibody Complex↗
PubMed1989

[Selected immunologic parameters in uveitis].

The behaviour of selected immunological parameters was checked in 38 patients with uveitis. The presence of immunological complexes was found in 23 patients and in 9 a positive Waaler-Rose's reaction. All patients showed a considerable fall in the percentage of the T lymphocytes with an increase of the B lymphocytes without markers as well as an increased concentration of the serum immunoglobulins of the M, A and G class.

Antigen-Antibody Complex↗
PubMed1988

Selected immunological parameters in patients suffering from uveitis subjected to different treatment.

Selected immunological parameters were studied in 70 subjects suffering from uveitis. The patients were treated with steroids, non steroid antiinflammatory drugs, and typhoid vaccine. After termination of treatment the following observations were noted: partial disappearance of immune complexes from circulation, increase of T lymphocytes and decrease of B lymphocytes percentage, decrease of IgG, IgM and IgA serum concentration, normalization of acute inflammation parameters (reduction of: ESR, leukocyte count, total protein and seromucoid concentrations; increase of albumin concentration).

Adolescent↗
PubMed1987

Immunostimulation in recurrent respiratory tract infections therapy in children.

Selected immunologic parameters and effectivity of immunotherapy was evaluated in 117 children (12-month-10-year-1-old) suffering from recurrent respiratory tract infections. All the children displayed a profound depression of T lymphocytes number, which resemble the situation seen in AIDS patients. An increase of serum IgM concentration was also noted. Immunotherapy included treatment with the following preparations: TFX and Levamisol which stimulate T cell functions, Broncho-Vaxom which stimulates specific antibody production and a complex herb preparation PADMA showing undefined general stimulatory activity. Separate group of children was subjected to climatotherapy in Czerniawa Sanatorium and received no immunostimulants. All methods of treatment employed had beneficial effect. The highest percentage of positive results was obtained in children receiving TFX and Levamisol. In all groups under study, an elevation of T cells percentage was observed. This was especially evident in Levamisol treated patients. There was no correlation, however, between T cells number and clinical improvement.

Adjuvants, Immunologic↗