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Biomedical subjects

E Yoshida

Publications and source records attributed to E Yoshida.

At least 127 records · Page 7Linked to original sources
PubMed1987

[A case report of a solid and cystic tumor of the pancreas in a 33-year-old woman].

An unusual case of a solid and cystic tumor of the pancreas is presented. A 33-year-old woman with a 15-year history of an abdominal tumor underwent a pancreatectomy. The tumor was encapsulated by a thick connective tissue and exhibited two main patterns: solid sheets of relatively uniform small cells; and, a papillary proliferation with a central fibrovascular talk. The tumor cells had PAS and d-PAS reactive granules in the cytoplasm. Immunohistochemically, a positive reaction for alpha l-antitrypsin and a carcinoembryonic antigen, as well as a weekly positive reaction for a vasoactive intestinal polypeptide also were found in the cytoplasm. Our results lead us to suggest that an S-C tumor is likely to be a tumor of a acinar cell origin.

Adult↗
PubMed1985

Inversely repeating integrated hepatitis B virus DNA and cellular flanking sequences in the human hepatoma-derived cell line huSP.

Among recombinant phages carrying integrated hepatitis B virus (HBV) DNA sequences cloned from the human hepatoma-derived cell line huSP, one clone, lambda hu-489, revealed some unusual features. The 2.25-kilobase Eco D fragment from the insert of this clone hybridized to the HBV DNA probe only and its nucleotide sequence was determined. The viral sequence, as well as a cellular flanking sequence, showed extensive rearrangement accompanied by inverted repetition. The Eco D fragment contained HBV DNA from the 5'-end region of gene S to the middle of gene X, followed by a long cellular flanking sequence. Moreover, a part of gene X was found inversely repeated at the head of the same gene S in a head-to-head configuration truncated by the same cellular sequence. Therefore, the same junction sequence of viral DNA and the cellular sequence was found at two different sites in the Eco D fragment in opposite polarities.

Base Sequence↗
PubMed1985

Hepatitis B virus integration in hepatocellular carcinoma DNA: duplication of cellular flanking sequences at the integration site.

The integrated form of hepatitis B virus (HBV) in the human hepatoma cell line huH2-2 and its cellular counterpart sequence have been cloned and analyzed. Blot hybridization analysis and nucleotide sequencing indicated that a single copy of the 1895-base-pair (bp) subgenomic region of HBV DNA, spanning from the middle of pre-S to the end of gene X, was integrated and flanked by the 12-bp directly repeating cellular sequences. A comparison of the sequencing data with that of the cellular counterpart DNA indicated the absence of deletion and rearrangement in the cellular flanking DNA following integration of the 1895-bp HBV DNA, except for generation of the 12-bp direct repeat at the virus-cell junction. A possible model for the mechanism of HBV integration is proposed.

Base Sequence↗
PubMed1985

[Clinical and immunological study on the high incidence of malignant neoplasms in patients with chronic renal failure].

With the advancement of dialysis techniques, rehabilitation and high morbidity are becoming serious problems for patients with chronic renal failure. The authors, with the purpose of investigating the carcinogenic state of such patients, studied the incidence of malignant neoplasms and their immunity both clinically and fundamentally. Out of 499 patients with chronic renal failure, malignant neoplasms were found in 17 cases (3.40%), which is obviously a high rate with a statistically significant difference from that of the ordinary healthy population. In the fundamental immunity study, OKM1+ cells of the patients were significantly lower than those of healthy people, and a decrease in NK and K cells was inferred. Incidentally, the lymphocytes of patients strongly inhibited the mitogen response of healthy subjects. This decline in cellular immunity is considered to be closely related to the carcinogenicity of patients with chronic renal failure.

Adult↗
PubMed1984

Electrocardiographic abnormalities in cerebrovascular accidents.

In order to assess the relationship of electrocardiographic (ECG) changes in acute cerebrovascular accidents (CVA) to the locations of the cerebral lesions, ECG records and brain computerized tomographic (CT) scans which were taken within 48 hours of CVA onset were examined in 39 patients without heart disease and/or treatment with cardiovascular drugs. The ECG was normal in 25% (3/12) of basal ganglial CVA, 22% (2/9) of frontal CVA and 36% (4/11) of temporo-parietal CVA. Nonspecific ST-T changes occurred in basal ganglial CVA (25%), frontal CVA (22%), temporo-parietal CVA (18%) and in the other categories of CVA locations. Corrected QT interval (QTc) elongation was observed not only in frontal (22%), but also in basal ganglial CVA (25%), temporo-parietal CVA (36%) and cerebellar CVA (50%). There were no ECG changes specific to a particular CVA location. These findings suggest that widely distributed neurons and pathways within the central nervous system may influence ECG.

Adult↗
PubMed1984

Direct evidence for the expression of integrated hepatitis B virus DNA in human hepatoma tissue.

The intracellular structure and expression of integrated hepatitis B virus (HBV) DNA in the hepatoma tissue #1707 obtained from an 11-year-old boy were studied. It was found that most of the HBV genome was present in the #1707 DNA. Virus-specific 21S mRNA was also demonstrated by blot hybridization. This is the first direct evidence for the expression of the integrated viral genome in human hepatoma tissue.

Base Sequence↗
PubMed1983

Rearrangement of the surface antigen gene of hepatitis B virus integrated in the human hepatoma cell lines.

The rearrangement of integrated HBV DNA sequences in three different hepatoma cell lines, huH-1, huH-2, KG-55-T from Japanese patients, were studied by blot hybridization using whole HBV genome or a HBsAg or HBcAg DNA as a probe. The characteristic existence of multiple integration sites of HBV DNA sequences in each HindIII-restricted hepatoma cell DNA was revealed by the HBV genome probe. Detection of the isolated HBsAg gene in the HindIII fragment indicates that the integration of HBV DNA was not always related to the maintenance of the whole viral genome, and that movement of the HBsAg gene to another location occurred by rearrangement. On the other hand, the presence of the HBV DNA sequence without the intact HBcAg gene was shown in some of the HindIII fragments, when the HBcAg gene, probe was used, but a HindIII fragment, containing only the HBcAg gene, was not detected so far. The absence of the intact HBcAg gene suggests that the viral genome may lose a part of the HBcAg gene in the process of integration. This is consistent with recent findings of Ogston et al. (1982) that in Woodchuck hepatocellular carcinoma viral sequences are extensively rearranged.

Base Sequence↗
PubMed1983

Tumor-associated mutations of rat mitochondrial transfer RNA genes.

Mitochondrial DNA is a sensitive target of chemical carcinogens (Backer and Weinstein (1980) Science 209, 297-299), suggesting that mutations of the mitochondrial genome occur in tumor cells. We examined this point by comparing mitochondrial DNA sequences in four rat tumors with those of normal rat liver. Some novel mutations found in the tRNA genes of tumor mitochondria were as follows: nucleotides deletions in the aminoacyl-acceptor stem of the tRNATyr gene or in the anticodon stem of the tRNATrp gene and insertions in the "YpsiC" loop of the tRNACys gene. These structures are extraordinary compared with those of the tRNA genes of other mammals, indicating that these mutations are each associated with a corresponding tumor.

Animals↗
PubMed1982

Nucleotide sequence and evolution of the rat mitochondrial cytochrome b gene containing the ochre termination codon.

The nucleotide sequences of the genes for cytochrome b and three potential transfer RNAs (tRNAPro, tRNAThr and tRNAGlu) in cloned rat mitochondrial DNA were determined. The derived amino acid sequence of the cytochrome b protein from the light strand indicated that the C-terminal amino acid is asparagine and the ochre termination codon is encoded in the DNA, in contrast to the the lack of termination codon in the reading frame of human [Anderson et al., Nature 290 (1981) 457] or mouse [Bibb et al., Cell 26 (1981) 167] mitochondrial DNA. The first ATG codon of the cytochrome b gene was spaced five nucleotides from the 5'-end of the tRNAGlu gene on the heavy strand. There was a single nucleotide spacing between the termination codon of the cytochrome b gene and the 5' end of the tRNAThr gene in the light strand. There was also a single nucleotide spacing between the 3'-end of the tRNAThr gene and the 3'-end of the tRNAPro gene on the heavy strand. The amino acid and nucleotide sequences of the cytochrome b genes of mammals and yeast [Nobrega and Tzagoloff, J. Biol. Chem. 255 (1980) 9828] were compared to reveal structural differences in two very different species. At the same time, amino acid substitutions in particular regions of the mammalian gene corresponding to the exon-intron boundaries in the yeast gene were noted. These genetic features are discussed in relation to the extreme compression of genetic information in the mammalian mitochondrial genome as related to the evolution of the gene organization and its sequence.

Amino Acid Sequence↗