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Biomedical subjects

E Zampachová

Publications and source records attributed to E Zampachová.

4 recordsLinked to original sources

[Epidemiologic monitoring of tick-borne encephalitis in Rimov in Southern Bohemia].

Southern Bohemia is one of the extensive natural foci of tick-borne encephalitis (TE) in the Czech Republic. Some professionals hold the view that people living in the focus of infection acquire naturally herd immunity and are the protected against the infection and thus it is not necessary to vaccinate them against TE. Several serious cases of the disease in temporal sequence in Rímov in the district of Ceské Budĕjovice led the authors to examine more closely the problem of TE in this locality. Available epidemiological data were analyzed. A group of subjects with permanent residence in the community was assembled who were interested to participate in the trial. In these serological examinations were made to assess antibodies against TE. Analysis of the assembled data proved that during permanent residence in Rímov 6.07% of the investigated group during their life in Rímov developed the manifest form of TE, 9.64% developed the inapparent form and 15% subjects had antibodies after previous vaccination. The ratio of manifest and inapparent cases is 6.07:9.64, i.e. roughly 2:3. The total seroprevalence after the disease (manifest and inapparent) is 16%. Persons with a negative result of the examination were offered vaccination. The vaccination rate increased due to the authors' intervention from 15% to 65% in the given community.

Adolescent↗

[Diagnosis of Chlamydia trachomatis by culture and Macchiavella's staining technique].

The author modified and tested staining according to Macchiavelli for detection of C. trachomatis in tissue cultures. As compared with IF staining by the monoclonal antibody the author found a high sensitivity (95.7%) and specificity (99.0%) of this method. Giemsa staining evaluated in passing light has a lower sensitivity (85.5%) and lower specificity (94.1%). The advantage of Macchiavelli staining is also the low price of the chemicals used. Imunofluorescent staining with the monoclonal antibody remains also now the reference method and is most reliable. For some materials which are obtained with difficulty (e.g. punctates etc.) the author recommends to use the IF technique.

Bacteriological Techniques↗

[Serologic detection of sexually transmitted diseases caused by Chlamydia trachomatis].

163 patients were examined for the presence of C. trachomatis by cultivation and concurrently sera were examined by the ELISA method for the presence of antibodies against C. trachomatis. Significant titres of antibodies were detected in less than half the patients with infection proved by cultivation. Antibodies were found more frequently in patients with a complicated course of the disease. In approximately 3% of the patients, where the agent was not detected by cultivation, antibodies were found in high titres. The author evaluated also the possibility to use the complement fixation reaction with ornithose antigen for the diagnosis of sexually transmitted infections caused by C. trachomatis. According to the author's results this method cannot be used. Serological evidence of Chlamydia infection is not a method of first choice for the diagnosis of sexually transmitted infections caused by C. trachomatis, but is an important supplementary method for the diagnosis, mainly of complicated infections.

Antibodies, Bacterial↗