Biomedical subjects

Eberhard Gwinner

Publications and source records attributed to Eberhard Gwinner.

21 records · Page 2Linked to original sources

PubMed2002

Non-invasive methods to measure androgen metabolites in excrements of European stonechats, Saxicola torquata rubicola.

Traditionally androgen concentrations are measured invasively in blood plasma. However, non-invasive methods to detect androgens are desirable, as this reduces interference with the natural behavior of the study species and multiple samples can be obtained relatively easy. The aim of this study was to validate a method to measure androgens non-invasively in excrements of male European stonechats (Saxicola torquata rubicola). Extracts of excrements of a male stonechat injected with [3H]testosterone ([3H]T) were chromatographically separated using high performance liquid chromatography (HPLC). The resulting HPLC fractions were then analyzed with a radioimmunoassay against testosterone (T-RIA). The results showed that the assay picked up major metabolites of [3H]T. The physiological relevance of excreted androgen metabolites was further validated by showing that injection of exogenous GnRH to seven males led to a significant increase in excreted androgen metabolites. In contrast, androgen metabolite levels of six saline-injected control males did not increase. Furthermore, excrements from nine males were collected from January until April to see whether the typical seasonal increase in testosterone levels can also be traced when measuring excreted androgen metabolites. As expected, there was a significant seasonal increase in androgen metabolite concentrations. Thus, the T-RIA measures androgen metabolites in droppings of male European stonechats and to our knowledge this study represents the first validation of a non-invasive androgen assay in a passerine bird.

Animals↗

PubMed2002

Spatial and temporal variation of passer Per2 gene expression in two distinct cell groups of the suprachiasmatic hypothalamus in the house sparrow (Passer domesticus).

In mammals, the major pacemaker controlling circadian rhythmicity is located in the hypothalamic suprachiasmatic nuclei. Although there is evidence for the presence of a hypothalamic circadian oscillator in birds from lesioning studies, neuroanatomical, neurochemical and functional investigations have failed to identify its exact location. Two cell groups in the avian hypothalamus have been shown to bear characteristics of the mammalian suprachiasmatic nucleus: the suprachiasmatic nucleus and the lateral hypothalamic retinorecipient nucleus. We cloned an avian period homologue (pPer2) and investigated the temporal and spatial expression pattern of this gene in the house sparrow hypothalamus using in situ hybridization. Applying quantitative morphometry, we found rhythmic expression of pPer2 during light-dark as well as in constant conditions in the suprachiasmatic nucleus and in the lateral hypothalamus. The temporal and spatial distribution of pPer2 expression in the suprachiasmatic nucleus suggest a longitudinal compartmentalization of the nucleus with period gene expression being initiated in the most rostral portion of the suprachiasmatic nucleus before lights on. In the lateral hypothalamus, phasing of pPer2-rhythmicity appeared different from the suprachiasmatic nucleus. The major difference between light-dark and constant conditions was a decrease in the amplitude of pPer2 rhythmicity in the suprachiasmatic nucleus. Our data demonstrate that, unlike in mammals, Per gene expression in the suprachiasmatic hypothalamus of the house sparrow is not confined to a single cell group, indicating a more complex organization of the circadian oscillator in the hypothalamus of birds.

Animals↗

PubMed2002

Is food availability a circannual zeitgeber in tropical birds? A field experiment on stonechats in tropical Africa.

Equatorial stonechats (Saxicola torquata axillaris) in Africa are seasonal breeders like their temperate-zone conspecifics (S.t. rubicola). Their annual cycle in gonadal size and function is controlled by an endogenous circannual rhythmicity that has been shown to run for up to 10 years in a constant equatorial photoperiod under laboratory conditions, with a period deviating from 12 months. In nature, however, this rhythm is synchronized with the actual year. Because photoperiod is essentially constant at the equator, it is likely that other environmental factors act as zeitgebers. The authors test whether food availability affects reproductive cycles of free-living East African stonechats. The authors offered supplemental food to the birds 2 months before the regular onset of the breeding season. Supplementally fed males started to sing and display earlier than males of control pairs that did not receive extra food. Although the supplemented food advanced the onset of the breeding season in the pairs that were fed, the onset of the postnuptial molt following the breeding season was not correspondingly shifted. Furthermore, in the year following the experiment, all pairs initiated breeding at the same time. The authors conclude that food availability does not act as a zeitgeber, but rather as a factor that modifies the timing of reproduction without affecting the underlying rhythmicity. The authors propose that this is adaptive under environmental conditions that are relatively constant within a given year but may vary considerably between years. The zeitgeber synchronizing the endogenous rhythmicity remains to be identified.

Animals↗