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OBJECTIVE: To develop a standard method for measuring tear production in large Psittaciformes using the phenol red thread (PRT) tear test. To establish mean PRT tear test values in clinically normal pet birds and to assess the reproducibility of the test. ANIMALS: Two geographically distinct populations of healthy, large Psittaciformes (pilot study and study population). Species groups include: cockatoo (17), macaw (22), Amazon (10), African gray (1), eclectus (1) and pionus (1). PROCEDURE: The PRT was placed under the upper eyelid and both eyelids were held closed throughout the test. The PRT tear test was repeated after 2 months in the pilot study and after approximately 5 min in the study population. RESULTS: The mean PRT tear test values for the pilot study were OD=28.2 mm/15 s (+/- 6.3 mm), OS=24.1 mm/15 s (+/- 6.6 mm) and OD=25.4 mm/15 s (+/- 3.3 mm), OS=25.5 mm/15 s (+/- 5.2 mm), for the first and second visits respectively. The mean PRT tear test value for the study population was: OD=19.8 mm/15 s (+/- 4.3 mm), OS=20.1 (+/- 3.9 mm) and OD=20.0 mm/15 s (+/- 4.5 mm), OS=19.1 mm/15 s (+/- 3.3 mm), for the first and second tests respectively. There was poor repeatability between tests. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the PRT tear test is a practical method for measuring tear production in large Psittaciformes, although adaptations to the established diagnostic method are necessary to overcome some anatomical differences present in birds. Good reproducibility of the PRT tear test could not be demonstrated in a clinically normal population of birds but geographic location appears to significantly influence results.