Biomedical subjects

Elzbieta Ronin-Walknowska

Publications and source records attributed to Elzbieta Ronin-Walknowska.

10 recordsLinked to original sources

PubMed2006

Maternal adipose tissue, maternal and cord blood essential fatty acids and their long-chain polyunsaturated derivatives composition after elective caesarean section.

BACKGROUND: The essential fatty acids and their long-chain polyunsaturated derivatives (LC PUFAs) are important for the growth and maturation of the fetal organism. OBJECTIVE: The aim of a study was to assess the profile of the essential fatty acids and their LC PUFAs in the blood and adipose tissue of pregnant women and in the cord blood after elective caesarean section. DESIGN: The study group comprised 36 women and 38 their full-term babies born after elective caesarean section. In maternal adipose tissue, maternal blood (separately in plasma and erythrocytes) and cord blood (separately arterial and venous) composition of the fatty acids in total lipids was estimated with gas chromatography. RESULTS: In the maternal plasma and erythrocytes the weight percent content of linoleic acid and alfalinolenic acid was higher and of LC PUFAs was lower than in plasma and erythrocytes from the umbilical vein with the exception of EPA and DPA. In the maternal subcutaneous adipose tissue the content of alfalinolenic acid was higher and of linoleic acid and of LC PUFAs were lower than in maternal plasma and erythrocytes. There was no significant difference in the weight percent content of estimated acids between the venous and arterial umbilical blood. In the maternal plasma the weight percent content of linoleic acid, alfalinolenic acid and LC PUFAs was lower than in the maternal erythrocytes. CONCLUSION: Differences between maternal and cord blood essential fatty acids and their metabolites indicate that preferential placental transfer of n-6 and n-3 fatty acid metabolites or faster metabolic turnover of linolenic and arachidonic acids in the fetus than in mother is possible.

Adipose Tissue↗

PubMed2006

[Monoamnotic twin pregnancy. Multicenter study].

OBJECTIVE: The purpose of this study was to evaluate of perinatal mortality and morbidity of monoamniotic twins. STUDY DESIGN: This was a multicenter retrospective analysis of 26 monoamniotic twin gestations identified between 1985 and 2004 in a 3 perinatal departments. Of these 26 pregnancies, 11 women were admitted electively for inpatient fetal monitoring. Overall mortality rates, the risk of intrauterine fetal death and neonatal mortality and morbidity, pregnancy complications and fetal anomalies were calculated. RESULTS: Monoamniotic twin pregnancies were diagnosed reliably prenatally by ultrasound in 22 women and at delivery in 4 cases. Of the 26 gestations, spontaneous fetal losses before 22 weeks of gestation were 4 cases. The overall loss rate and the perinatal mortality rate were 52% and 19.4%, respectively. Twenty-two women had both twins alive at 24 weeks of gestation; 11 women were admitted electively for inpatient fetal monitoring at 26-27 week of gestation. In this group there were 2 neonatal deaths. No intrauterine fetal death occurred in any hospitalized patient. In our series there were 100% incidence of prematurity, 38,5% of umbilical cord entaglement, 23% of TTTS, 3.6% of TRAP and 9.6% fetal congenital anomalies. CONCLUSION: Monoamniotic twins are at extremly risk of pregnancy complications and fetal loss. These pregnancies can be diagnosed reliably by ultrasound in most cases. Electively admitted women for inpatient fetal monitoring could be improved neonatal survival and decreased perinatal morbidity.

Congenital Abnormalities↗

PubMed2004

[Pregnancy, labor and puerperium in patients with HELLP syndrome].

OBJECTIVE: The aim of this study was to evaluate retrospectively 10 women with the syndrome of hemolysis, elevated liver enzymes, low platelets (HELLP)--age of patients, quality of antepartum medical care, clinical, biochemical parameters, treatment, course and duration of pregnancies, way of delivery, and puerperium. MATERIAL AND METHODS: Women in the study group were divided in order of platelets (PLT) levels: 4 patients with PLT levels lower than 50 G/l and 6 patients with PLT levels higher than 50 G/l. The mean duration of pregnancies in both groups was similar. RESULTS: In the first group just one women delivered by vaginal route when compared with the cesarean delivery. We noticed lower frequency of antepartum medical visits among the first group. Such signs as vomiting, nausea, abdominal pain, pruritus occurred more frequently in the second group. The most common complication observed in both groups of patients was severe preeclampsia. HELLP syndrome was also observed in the multiple pregnancies. In the study group high blood concentrations of urea, creatinine, total bilirubin, alanine transaminase and aspartate aminotransferase were present. Termination of pregnancy is still recognised to be the best way of HELLP treatment, however the efficacy of steroid drugs administration as conservative treatment were also demonstrated. CONCLUSION: The disseminated intravascular coagulation as the HELLP complication in the puerperium period seems to be a serious problem, which can conduct to the need of hysterectomy.

Adult↗

PubMed2004

[Comparison of fetal ECG recordings using STAN S21 analyser and external ECG of newborns, in relation to enzymatic ischaemic markers and acid-base parameters in umbilical blood].

DESIGN: Neonatal hypoxia is often responsible for myocardial ischaemia. To evaluate cardiac involvement in neonates, fetal and neonatal ECG recordings have been performed and parameters indicating heart dysfunction have been analysed. OBJECTIVES: Our interest has been focused on analysis of fetal electrocardiogram recordings using STAN S21 analyser during labour, compared to newborns ECG recordings in the first 12 hours of life, in relation to enzymatic ischaemic markers and acid-base parameters in umbilical blood. MATERIALS AND METHODS: The data were collected from 8 term fetuses and neonates, continuously monitored in the first and second stage of labor. All ECG were recorded with a single spiral scalp electrode connected to the STAN S21 analyser. After birth, umbilical venous blood samples have been collected for determination of pH, base excess and concentrations of creatinine kinase (CK), its isoenzyme (CK-MB), myoglobin, lactate dehydrogenase (LDH), alanine transaminase, aspartate aminotransferase. In the first 12 hours of life, external neonatal ECG was performed. RESULTS: P,QRS and T waves have been present in all cases recorded. There was no suggestion of fetal hypoxia in any case. A little variety of QRS complex has been observed. In our study, we didn't noticed any pathological changes in ECG recordings. The QT interval, corrected by the Bazett formula was normal in all cases. Nevertheless correct ECG recordings, increase of enzymatic ischaemic markers (LDH, CK,CK-MB) has been observed in two cases, corresponding to the normal fetal outcome.

Acid-Base Equilibrium↗

PubMed2003

[Application of intravenous immunoglobulin therapy (IVIG) in pregnant patients with recurrent spontaneous abortions].

OBJECTIVE: Clinical outcome of pregnancy in patients with recurrent pregnancy losses treated with IVIG during their current pregnancy. MATERIAL AND METHODS: The study group consisted of 10 pregnant women with 3-5 spontaneous abortions. Any genetic, anatomical or infectious abnormalities were excluded as well as antiphospholipid antibody syndrome and negative lymphocytotoxic test. The treatment consisted of passive immunotherapy by means of intravenous administration of immunoglobulin (IVIG) at a dose of 0.4 g/kg b.w. Therapy was commenced at week 5-6 of gestation, infusions were repeated every 3-4 weeks until 28th week of gestation in 5-, 3- and 1-day protocols. In 2 patients that therapy followed active immunotherapy with paternal lymphocytes. RESULTS: Eight (80%) patients bore healthy babies (3 preterm); in 2 cases embryos died at 6-7 week of gestation. CONCLUSIONS: The results confirm the efficacy of IVIG in pregnant patients with recurrent spontaneous abortions in whom likelihood of immunological pattern of pregnancy failures is high.

Abortion, Habitual↗

PubMed2003

[The relationship between intrapartum fetal heart rate disturbances, electrocardiographic changes and umbilical venous troponin-I (T-I)].

OBJECTIVE: To analyse the relationship between intrapartum fetal heart rate disturbances and electrocardiographic changes and umbilical venous troponin-I (T-I), concentrations as well. MATERIAL AND METHODS: 14 fetuses were continuously surveyed by CTG and ECG recordings in the first and second stage of labor, using STAN S-21 analyser. After birth, umbilical venous blood samples were collected for determination of acid-base balance, base excess and troponin-I concentrations. RESULTS: From among monitored fetuses, in 8 cases the CTG and FECG patterns were correct. Vaginally delivered neonates were born in good clinical status, with normal acid-base balance, base excess and T-I < 0.3 ng/ml. In 3 cases abnormal CTG patterns were observed with early decelerations but FECG patterns were correct. Vaginally delivered neonates were born in good clinical status, with normal acid-base balance, base excess and T-I < 0.3 ng/ml. In 2 cases abnormal CTG patterns were observed with variable decelerations but FECG patterns were correct. Vaginally delivered neonates were born in good clinical status, with normal acid-base balance, base excess and T-I < 0.3 ng/ml. In one case abnormal CTG pattern were observed with late decelerations but FECG patterns was correct. The pregnancy was terminated by caesarean section because of fetal distress. The neonate was born in good clinical status with normal acid-base balance and base excess but T-I concentration was increased (1.5 ng/ml).

Acid-Base Equilibrium↗

PubMed2002

The effect of thymus ubiquitin complex on neuropathological changes within the brain of pregnant rabbits with experimental model of antiphospholipid syndrome.

The present study was designed to assess the impact of thymus ubiquitin complex (TUC) on morphological changes within the central nervous system (CNS) in pregnant rabbits with experimental antiphospholipid syndrome (APS). We also compared the intensity of neuropathological appearance and serological markers of the APS treated with the TUC. Post-mortem neuropathological investigations were done in 47 female New Zealand rabbits. The material was divided into 4 groups: Group I-23 pregnant animals with APS; Group II-7 pregnant rabbits with APS, treated with TUC; Group III-7 pregnant rabbits without APS, treated with TUC; Group IV (the control one)-110 pregnant animals without APS, untreated with TUC. The APS was induced by subcutaneous injections of cardiolipin. There were two main abnormalities within the CNS of pregnant rabbits, which could be a result of the APS: the thrombo-necrotic foci of nervous tissue, and perivascular or meningeal inflammatory infiltrates. It turned out that TUC application reduced the morphological abnormalities, but it did not eliminate them entirely. The number of cases with thrombo-necrotic changes was reduced by approximately 18%, while perivascular infiltrates by 36% respectively. The TUC application did not influence the meningeal infiltration. The platelet count significantly increased (p < 0.001), the activated partial thromboplastin time shortened (p < 0.001), and the number of immunised animals, demonstrating positive immunofluorescence test, significantly decreased after the TUC administration. The inflammatory changes within the CNS are the integral component of the experimental model of APS in pregnant rabbits. The TUC may decrease the inflammatory component of the APS in the CNS. The inflammatory infiltrates seem to be more responsive to TUC application and parallel to APS serological improvement after treatment than necrotic changes.

Animals↗

PubMedDate not supplied

[Immunological aspects of premature termination of pregnancies].

In the presented article we report hypotheses, opinions and confirmed results of investigations concerning immunological mechanisms, that play a role in the physiological pregnancy maintenance, natural reproduction failure and artificial reproductive techniques (ART) as well as abilities to diagnose immunological disorders. We pointed the important role of histocompatibility antigens such are class I and class II major histocompatibility antigens with their soluble forms (HLA-I; HLA-II), DQ-alpha and DQ-beta alleles as well as non classic antigens: HLA-G, HLA-C, HLA-E, in modulation of cellular and humoral response, which represent potential alloimmunological mechanism for pregnancy losses. The accurate report of immunological mechanism for recurrent spontaneous abortions caused by maternal production of phospholipid antibodies in antiphospholipid syndrome (APS) or other antibodies implicated as etiological factors in reproductive autoimmune failure syndrome (RAFS) has been demonstrated. We present the utility of identification of, so-called, reproductive immunophenotype, where an increase of some serum lymphocyte subpopulations anticipating pregnancy loss is frequently observed. In the study we describe recommendations and diagnostic capabilities for detection of immunological disturbances in patients undergoing evaluation for repeated pregnancy loss, which could be helpful in choosing the form of therapy.

Abortion, Spontaneous↗

PubMedDate not supplied

[The significance of HLA-G for miscarriages].

In the article, we described major histocompatibility antigens and their distribution in the feto-maternal junction. In particular we have taken into consideration HLA-G, its structure and isoforms. Further on, we cited opinions about their role during reproductive processes, creation of syncytiotrophoblast, remodeling of spiral arteries, growing and maturation of placenta. The lack of correct function of HLA-G can lead to disorders in ovum implantation, hormonal and nutritional function of placenta which can cause reproductive failure, including abortions, intrautrine growth retardation, intrauterine fetal demise, preeclampsia. The genotype HLA-G is potentially related to habitual abortions.

Abortion, Spontaneous↗

PubMedDate not supplied

[Caesarean section in preterm labour].

The aim of this study was to estimate complications of pregnancy, indications to caesarean section, complications after caesarean operation and perinatal mortality of newborns who were delivered before the 37th week of gestation by caesarean section. The retrospective analysis of the total of 2693 deliveries (years 1991-2000) by caesarean section in the Department of Pregnancy and Labour Pathology in Pomeranian Academy of Medicine has been carried out. 590 women whose delivered before the 37th week of pregnancy have been chosen for subsequent examination. The patients have been divided into three groups: group I--patients with pregnancy completed before the 28th week, group II--patients with pregnancy completed between 29th and 32nd week, and group III--patients with pregnancy completed between the 33rd and 36th week. Obstetrical anamnesis, complications of pregnancy, indications to caesarean section and postoperative complications as well as newborns perinatal mortality have been analysed. Among women who experienced abortions, premature deliveries caesarean section a higher rate of caesarean section during subsequent premature delivery was stated. The increase of caesarean sections rate in premature deliveries after the 29th week of gestation is associated with a considerable decrease in newborns, mortality.

Cesarean Section↗