Biomedical subjects

Encarnacion Moreno

Publications and source records attributed to Encarnacion Moreno.

4 recordsLinked to original sources

PubMed2006

A selective differential medium for Lactobacillus plantarum.

The quantification of exogenous lactobacilli in faecal samples is frequently required for the evaluation of the intestinal colonization by probiotic bacteria. In this study, a selective and differential medium, designated LPSM, was developed for the culture of exogenous Lactobacillus plantarum. In quantitative assays, LPSM showed a sensitivity similar to those of enriched and Lactobacillus-adapted media. The presence of ciprofloxacin made LPSM inhibitory to most intestinal bacteria, including endogenous acid lactic bacteria, whereas exogenous L. plantarum strains grew producing a yellow color caused by acid production from sorbitol in the presence of bromocresol purple. The results showed that LPSM is suitable for detection and enumeration of L. plantarum in faecal samples.

Animals↗

PubMed2005

Immunomodulation by non-absorbable antibiotics given by the intragastric route.

To test the role of bacterial fractions released from intestinal flora during immunomodulation by antimicrobial agents, BALB/c mice were treated with the non-absorbable antibiotics polymyxin B or teicoplanin by the intragastric route. The composition of faecal microbiota and the capacity of spleen cells to proliferate in response to B-cell and T-cell mitogens were assessed at several times during the treatment. Both antibiotics lowered the count of some bacteria of the intestinal flora and induced significant modifications in spleen cell ability to proliferate in response to mitogens. Thus, the active fractions released from intestinal bacteria during antibiotic treatments may be able to induce immunomodulating effects.

Animals↗

PubMed2003

Immunomodulation by Yersinia enterocolitica: comparison of live and heat-killed bacteria.

This study compared the immunomodulating properties of viable and killed Yersinia enterocolitica O9 in BALB/c mice. At 10 days after infection by the intragastric route, ex vivo assays showed a suppression of spleen cell proliferation in response to Salmonella lipopolysaccharide, concanavalin A and heat-killed yersiniae. Mice infected with Y. enterocolitica O9 for 10 days resisted the challenge with a lethal dose of Listeria monocytogenes. In contrast, intravenous administration of heat-killed yersiniae did not modify the ability of spleen cells to proliferate in response to lipopolysaccharide or concanavalin A, and proliferation in response to killed yersiniae was significantly increased. By 3 days after administration of a single dose of heat-killed yersiniae, the resistance of mice to L. monocytogenes challenge was significantly increased. Our findings show profound differences in immunomodulation by viable and heat-killed yersiniae, but suggest that killed yersiniae retain interesting immunomodulating properties.

Animals↗

PubMed2003

Modification of mitogen-driven lymphoproliferation by ceftriaxone in normal and immunocompromised mice.

Cyclophosphamide-treated mice are proposed as a model to assay immunomodulation by antimicrobial agents in immunocompromised animals. Cyclophosphamide-treated BALB/c mice developed temporary leukopenia, myelopenia and spleen atrophy that was followed by splenomegaly. Cells from both atrophic and hypertrophic spleens exhibited impaired responses to mitogens and suppressed the mitogen-driven proliferation of normal splenocytes. This experimental model was applied to the study of immunomodulation by ceftriaxone. Ceftriaxone did not worsen the cyclophosphamide-damaged immunity mechanisms. On the contrary, cyclophosphamide-induced suppression of lymphoproliferation in response to concanavalin A was attenuated by ceftriaxone.

Adjuvants, Immunologic↗