Analysis of chromosomes 8 and 17 aneuploidies in laryngeal squamous cell carcinoma by fluorescence in situ hybridization.
OBJECTIVES/HYPOTHESIS: The objectives were to investigate chromosomes 8 and 17 numerical aberrations by using fluorescence in situ hybridization in laryngeal squamous cell carcinoma and also to determine whether there is any association between chromosomes 8 and 17 aneuploidies and TNM classification and subgroups of laryngeal squamous cell carcinomas. STUDY DESIGN: Descriptive study. METHODS: Tumor and control samples were taken from 23 patients with LSCC by surgical operation. Fluorescence in situ hybridization analysis with chromosomes 8- and 17-specific alpha-satellite DNA probes was performed on the interphase nuclei. RESULTS: The percentages for chromosomes 8 and 17 aneuploidies were 33% (SD = 25.7%) (median value, 26.2%; range, 3.5%-81.8%) and 19.2% (SD = 15.8%) (median value, 9.8%; range, 3.6%-63.7%), respectively. There was a significant difference between stage 2 and stage 3 (P <.05) and between stage 2 and stage 4 for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 aneuploidy (P >.05). There was also a significant difference for the T classification for chromosome 8 aneuploidy (P <.05) but not for chromosome 17 (P >.05). CONCLUSION: Chromosome 8 aneuploidy may be related to stage and T classification of laryngeal squamous cell carcinoma and its progression.