Eric W Lloyd | PubMed Health

PubMed2006

Publication rates of scientific presentations at the American Association of Hip and Knee Surgeons annual meetings from 1996 to 2001.

National orthopedic subspecialty meetings are a research forum where volumes of investigations are presented after a peer-reviewed selection process. The objective of this investigation was to determine the publication rate of recent scientific presentations presented at the American Association of Hip and Knee Surgeons (AAHKS) annual meetings. From 1996 to 2001, 168 (58%) of 292 podium abstracts presented at meetings were published. The average time to publication was 21.7 +/- 14.7 months. Three major orthopedic journals constituted 89% of the publications. The 58% publication rate for AAHKS ranks as one of the higher rates for orthopedic specialty meetings and validates the selection process for abstracts presented. In addition, AAHKS meetings are an excellent source for a wide array of quality research and scientific information.

Bibliometrics↗

PubMed2002

The READIT assay as a method for genotyping NAT1*10 polymorphisms.

Polymorphisms in the N-acetyltransferases (NATs) have been associated with increased risks for the development of a variety of cancers. The NAT1*10 allele, for example, has been reported to be associated with an increased risk of colon and urinary bladder cancers, among others. Therefore, considerable effort is being placed on the development of genotyping methodologies for NAT activities both for pharmacological as well as disease preventative applications. Most NAT genotyping approaches are gel based and consist of PCR-restriction fragment length polymorphism (RFLP) analysis, allele-specific PCR, or both. Although these approaches have their utility, they are slow, labor intensive, and are not amenable to automation. Recently, a novel approach to genotyping known as the READIT Assay has been introduced. The READIT methodology involves a reversal of the DNA polymerase reaction to generate dNTPs through the phosphorolytic cleavage of oligonucleotide probe molecules annealed to target DNAs. In a coupled reaction, kinase converts the resulting dNTPs to ATP. ATP production is then monitored by the addition of luciferase, generating a light signal proportional to the amount of dNTPs generated through probe depolymerization. We describe the development of a READIT genotyping protocol for the analysis of NATs using the NAT1*10 allele as a model system and demonstrate its utility for the analysis of archival dried blood specimens. We applied this technology to genotype 678 DNAs at the NAT-1088T --> A polymorphic site, and 680 DNAs for the 1095C --> A polymorphism. We report complete concordance for the 1088T --> A polymorphism for all 678 genotypes previously determined by RFLP analysis.

Arylamine N-Acetyltransferase↗