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Bibliography of Medicine↗
Biomedical subjects
Ernesto Castelazo Morales
Publications and source records attributed to Ernesto Castelazo Morales.
[Initial values of human chorionic gonadotropin as a prognostic variable of pregnancy evolution in assisted reproduction].
INTRODUCTION: In assisted reproduction programs, once a pregnancy has been achieved, the power of predicting its evolution is important for the medical team as well as for the patient. The determination of pregnanediol or of placental protein 14 levels has been postulated as a predictive factor, however, the determination of hCG is, seemingly, the most adequate parameter for the detection of early developmental alterations during pregnancy. OBJECTIVE: To determine those predictive values of hCG which establish a prognosis concerning the evolution of pregnancy through the analysis of the values of this hormone on post-transference days 14 and 16, as well as to determine hCG values for multiple pregnancies. MATERIAL AND METHODS: Data from 88 patients participating in the in vitro fertilization program, in whom pregnancy was achieved, were retrospectively analyzed, and hCG levels were determined on post-transference days 14 and 16. Results were analyzed by the ANOVA test in order to compare mean values, & ROC were used to determine sensitivity and specificity. RESULTS: The patients were divided in three groups: 1) pregnant for more than 26 weeks; 2) pregnant for less than 26 weeks; and 3) chemical pregnancy. Significant differences in hCG levels on post-transference days were observed among these groups of patients. The levels of hCG, on post-transference day 14, 113 IU/L, and on day 16, 403 IU/L, presented, respectively, an 80% and 79% specificity, while the greater than 200.33 IU/L hCG increase offers a sensitivity and specificity of 80% and 83%, respectively, for a pregnancy of more than 26 weeks. In order to consider hCG levels as predictive for a high fetal order pregnancy, it was observed that hCC levels of 270.42 UI/L on day 14 present a sensitivity of 91% with a specificity of 64%, and levels higher than 837.14 IU/L on day 16 offer a sensitivity and specificity of 91% and 62%, respectively. CONCLUSIONS: The determination of hCG levels greater than 113 IU/L on day 14 and greater than 403 IU/L on day 16 offer adequate sensitivity and specificity as predictive factors for pregnancy evolution beyond the 26 GW, while hCG levels of 270.42 and 837.14, respectively, on days 14 and 16 offer a very high sensitivity (91%) for multiple pregnancy.
Adult↗
[Changes in mitochondrial membrane potentials and its exponential relation with phosphatidylserine translocation in the plasma membrane as markers in the initial events of apoptosis: evaluation in different spermatic fractions].
OBJECTIVE: To determine the integrity of the plasmatic membrane through phosphatidylserine (PS) translocation in two spermatic fractions and their correlation with the spermatic mitochondrial membrane potential. MATERIAL AND METHODS: The analysis of both spermatic fractions was carried out through a discontinuous gradient separation with Percoli, in order to obtain two samples with high and low mobility (90-40%). Twelve patients were recruited for the initial evaluation of seminal parameters. Mitochondrial membrane integrity was determined using a second antibody (Mitosensor), and was analyzed by fluorescence microscopy, evaluating an average of 200 cells. A 450-490 nm excitation filter was used for this analysis. Cytoplasmatic assessment was carried out by anexine V bonding to PS, in order to determine the initial events of cellular death. Non parameter tests were used in order to determine the differences between mitochondrial potentials and plasmatic membrane processes. Linear correlation tests were used for the anexine V and Mitosensor ratios. RESULTS: Due to the study's design, some differences were observed regarding the displacement parameters and the presence of apoptosis, both, in the plasmatic membrane and in mitochondrial membrane potentials. A positive correlation between both, mitochondrial and cytoplasmic membrane functions was also found. CONCLUSIONS: This is the first study performing a comparative analysis between mitochondrial membrane function and cytoplasmatic PS expression as early cellular death markers. The male infertility population is probably associated with an increase in this kind of apoptosis processes.
Apoptosis↗